Sequencing the gigabase plant genome of the wild tomato species Solanum pennellii using Oxford
Nanopore single molecule sequencing
Early MinION ™
nanopore single - molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples — Virginie Sauvage — PLOS ONE
Not exact matches
• Oxford
Nanopore Technologies Ltd, a U.K. - based developer of nanopore - based electronic systems for analysis of single molecules, raised $ 100 million pounds ($ 140 m
Nanopore Technologies Ltd, a U.K. - based developer of
nanopore - based electronic systems for analysis of single molecules, raised $ 100 million pounds ($ 140 m
nanopore - based electronic systems for analysis of
single molecules, raised $ 100 million pounds ($ 140 million).
UK - based Oxford
Nanopore Technologies published a report last year showing that it could detect methylated DNA at a
single - molecule level.
Nanopore sequencing promises to do away with these added steps by sequencing
single unmodified DNA strands, and thereby possibly becoming the fastest and cheapest sequencing method on the market.
At left is a
single nanopore viewed from the side; at right is a diagram of metabolite exchange across the membrane.
Drndić and her colleagues tested their silicon nitride
nanopore on homopolymers, or
single strands of DNA with sequences that consist of only one base repeated several times.
An illustration of a
single - stranded DNA homopolymer translocating through a silicon nitride
nanopore.
Now, a team led by University of Pennsylvania physicists has used solid - state
nanopores to differentiate
single - stranded DNA molecules containing sequences of a
single repeating base.
In this experiment, Drndić and her colleagues worked with a different material — silicon nitride — rather than attempting to craft
single - atom - thick graphene membranes for
nanopores.
Background The MinION ™ is a new, portable
single - molecule sequencer developed by Oxford
Nanopore Technologies.
A reference bacterial genome dataset generated on the MinION ™ portable
single - molecule
nanopore sequencer
The audience fidgeted with eager impatience to hear Clive Brown of Oxford
Nanopore present an update on their
single - molecule, real - time sequencing technology.
Andre Marziali, Ph.D., University of British Columbia, Vancouver $ 650,000 (3 years) «
Nanopores for Trans - Membrane Bio-Molecule Detection» This group will contribute to understanding how
single biological molecules interact with pores inserted into membranes, and how useful information can be derived from those interactions.
This week KeyGene researchers generated 76 gigabases of DNA sequence of a lettuce (Lactuca sativa) genome with a
single Oxford
Nanopore Technologies (ONT) PromethION flow cell.
New generation of sequencing technology uses
nanopores to deliver ultra long read length
single molecule sequence data, at competitive accuracy, on scalable electronic GridION platform.
The design and engineering of advanced solid - state
nanopore materials could allow for the development of novel gene sequencing technologies that enable
single - molecule detection at low cost and high speed with minimal sample preparation and instrumentation.
Motivation:
Single Molecule Real - Time (SMRT) sequencing technology and Oxford
Nanopore technologies (ONT) produce reads over 10kbp in length, which have enabled high - quality genome assembly at an affordable cost.
We have assembled de novo the Escherichia coli K - 12 MG1655 chromosome in a
single 4.6 - Mb contig using only
nanopore data.
Nanopore sequencing is a promising technique for genome sequencing due to its portability, ability to sequence long reads from
single molecules, and to simultaneously assay DNA methylation.
Nanopore - based
single molecule sequencing of the D4Z4 array responsible for facioscapulohumeral muscular dystrophy
The Oxford
Nanopore MinION is a portable
single - molecule DNA sequencer that can sequence long fragments of genomic DNA.
Speed,
single - base sensitivity and long read lengths make
nanopores a promising technology for high - throughput sequencing.
The MinION is a portable
single - molecule DNA sequencing instrument that was released by Oxford
Nanopore Technologies in 2014, producing long sequencing reads by measuring changes in ionic flow when
single - stranded DNA molecules translocate through the pores.
Background: Oxford
Nanopore Technologies Ltd (Oxford, UK) have recently commercialized MinION, a small single - molecule nanopore sequencer, that offers the possibility of sequencing long DNA fragments from small genomes in a matter of
Nanopore Technologies Ltd (Oxford, UK) have recently commercialized MinION, a small
single - molecule
nanopore sequencer, that offers the possibility of sequencing long DNA fragments from small genomes in a matter of
nanopore sequencer, that offers the possibility of sequencing long DNA fragments from small genomes in a matter of seconds.
Motivation:
Nanopore sequencing may be the next disruptive technology in genomics, due to its ability to detect
single DNA molecules without prior amplification, lack of reliance on expensive optical components, and the ability to sequence very long fragments.