Purified plasmid clones at each serial dilution were quantified using a Nanodrop spectrophotometer and then spiked into
negative serum, stool, or oral
swab sample matrix, each matrix consisting of a pool of 10 sera, 10 stool
samples, or 3 oral
swabs, respectively.
Group B Strep testing; streak testing for isolation and propagation of positive and
negative controls, inoculation of Lim broth with patient
sample, Multicenter Evaluation of the BD Max GBS Assay for Detection of Group B Streptococci in Prenatal Vaginal and Rectal Screening
Swab.