Immunocytochemical staining for Ki67 (cell proliferation marker), Sox2 (
neural stem cell marker), Olig2 (progenitor cell marker), and GFAP (glial fibrillary acidic protein, a marker for differentiated astrocytes) captured by confocal microscopy imaging.
Not exact matches
Intrigued, the team inserted a human version of L1 into rat
neural stem cells, along with a
marker that would make the
cells glow green whenever L1 made a jump.
Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic
stem cells with fibroblast growth factor 2 (FGF2) leads to
neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of
markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.
However, we have found a fluorescent
marker for nestin, a
neural crest
stem cell marker, in the posterior limbal region of the human anterior segment in the vicinity of both the trabecular meshwork and Schwalbe's line (unpublished).
Expression of the Wnt inhibitor Dickkopf - 1 is required for the induction of
neural markers in mouse embryonic
stem cells differentiating in response to retinoic acid.
(C) Confocal image of P150 dystrophic inner retina transplanted with hNPCctx — GDNF and double stained with antibodies against nestin, a
neural stem and progenitor
cell marker (green) and human nuclear antigen (red).
Neural crest - like stem cells (NCLSC) share many properties with aggressive melanoma cells, such as high migratory capabilities and expression of neural crest ma
Neural crest - like
stem cells (NCLSC) share many properties with aggressive melanoma
cells, such as high migratory capabilities and expression of
neural crest ma
neural crest
markers.
Of note, mouse
neural stem cells (NSCs) were reprogrammed using only one (Oct4) or two factors (Oct4 and Klf4), due to the endogenously high expression of pluripotent genes, such as Sox2 and c - Myc, as well as several intermediate reprogramming
markers [14], [17], [18].