Over the years the mutations and the cells they affect have led us to study new channel proteins, new transcription factors, neurodegeneration, microtubule function and structure,
neuronal outgrowth, insulin signaling, cellular ensheathment, and touch sensitivity to give just a partial list.
For example, the expression of BMP - Smad signaling pathway components is low during differentiation of mDA neurons from their VM neural precursors in vivo (E10 — E12), yet after this period, these subsequently increase in expression and play a functional role in mDA
neuronal outgrowth (E1 — E18)[37].
Cell - cell interactions are also important in a later stage of
neuronal outgrowth when processes grow into the region of their targets.
Neuronal outgrowths in 24 - well tissue culture dishes were fixed, permeabilized and blocked as above prior to staining with anti-β-III tubulin, 1 ∶ 200 (Chemicon mab1637 followed by secondary labeling with AF488 goat anti-mouse IgG1 (A21121, Invitrogen).
Not exact matches
In vitro, Pkn1 — / — Cgcs exhibited deregulated axonal
outgrowth, elevated AKT phosphorylation, and higher levels of
neuronal differentiation - 2 (NeuroD2), a transcription factor preventing presynaptic maturation.
Contactin genes CNTN5 and CNTN6 code for
neuronal cell adhesion molecules that promote neurite
outgrowth in sensory - motor
neuronal pathways.