300,000 cells were plated per well of a 24 - well plate (minimum 10 wells) and mixed with virus (80
ng p24 / 106 cells) diluted in 100 µl of fresh medium.
100
ng p24 of HIV - 1 was used per million cells.
Five days post-stimulation cells were infected with 100
ng p24 / million cells and then were cryopreserved four days post-infection.