Sentences with phrase «nitrocellulose membranes»
Gels were transferred onto nitrocellulose membranes with a semidry blotting system.
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Fifteen micrograms protein was run by SDS / PAGE and transferred to nitrocellulose membranes using a Mini Trans - Blot Electrophoretic Transfer Cell (Bio — Rad, CA, U.S.A.).
Equal amounts of protein or conditioned medium were separated by SDS — polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes and probed with the indicated antibodies.
Proteins were separated by SDS — PAGE and transferred on a nitrocellulose membrane.
The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes.
Lysates were separated via SDS - PAGE on a 12.5 % Bis - Tris polyacrylamide gel and transferred onto nitrocellulose membrane.
Proteins were transferred to a nitrocellulose membrane using a semidry electroblotting method [19,20].
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10 min at 70 °C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5 % BSA and probed with unpurified ab51608.
4) Nitrocellulose membrane.
Protein was separated on an SDS — PAGE and transferred to Hybond - C Extra nitrocellulose membrane.
After the sample and buffer are added to the sample well any antigens in the sample migrate across a nitrocellulose membrane along with the gold - labeled antibody (conjugate) and form an antigen / antibody gold complex.