Study the mechanistic role
of noncoding RNA molecules, in particular, circulating microRNAs as regulatory molecules that contribute to the onset and progression of AD.
Detecting selection in
noncoding regions of nucleotide sequences Wong, W. S. W. and R. Nielsen.
But full sequencing will soon be just as cheap as exome sequencing and will catch disease mutations
in noncoding regions as well, Gibbs notes.
In addition, some studies have found evidence that species from flies and worms to humans copy large amounts of RNA from
noncoding DNA, with no apparent purpose.
J H S Tong and colleagues report a study that brings into sharp focus the likely relevance of
noncoding variants for the genetic risk associated with Attention deficit hyperactivity disorder (ADHD).
And let's face it, exome sequencing lets us conveniently avoid some of the most challenging aspects of human genomics, like detecting complex rearrangements (SVs) and interpreting
noncoding regulatory variants.
The patterns of a type of molecule that influences epigenetic inheritance, called
small noncoding RNA, differed between the two groups, as did methylation patterns in the sperm DNA in the two groups, the team reports today in Cell Metabolism.
Sebat says the large
noncoding portion of our DNA — often previously referred to as «junk DNA» — has so far been ignored in autism research.
The novel long
intergenic noncoding RNA UCC promotes colorectal cancer progression by sponging miR - 143.
And this so -
called noncoding DNA plays a role in some diseases, studies have shown.
«The elephant results revealed
noncoding sequences in the human genome that we predict may control gene activity and reduce the formation of mutations and cancer.»
Clearly,
noncoding mutations affecting gene expression are of high interest in cancer genetics.
Two recently discovered genetic differences between brain cancer cells and normal tissue cells — an altered gene and a snippet of
noncoding genetic material — could offer clues to tumor behavior and potential new targets for therapy, Johns Hopkins scientists report.
In the June update, 278 retinal disease genes had been mapped, including first bona - fide
noncoding gene linked to a retinal disease.
Some of the transcripts hail
from noncoding DNA, the researchers report, but those that do match up with the 399 ENCODE genes overlap with each other extensively.
«It shows a very nice role
for noncoding RNA at the early embryonic stage for transmission of the transgenerational phenotype.»
Candidate genes and
functional noncoding variants identified in a canine model of obsessive - compulsive disorder.
Based on these results, Sebat proposes a more complex model of how autism arises, in which mothers pass on mutations affecting coding regions, which have large effects that women are protected from, while fathers pass on variants
affecting noncoding regions; their effects are much more moderate and may only cause symptoms when combined with risk variants from mothers.
The role of
most noncoding regions in health and disease remains unclear.
Like many genes, Dscam consists of protein - coding regions called exons interspersed
with noncoding regions.
Elevated amounts of a
viral noncoding RNA that suppresses host immunity likely led to an outbreak of dengue virus.
Jennifer Stauffer: Assessing the Function of Highly
Conserved Noncoding Elements in Drosophila melanogaster via Captured Segment Exchange
In a patient with Usher syndrome, the authors identified a known
pathogenic noncoding variant (intronic) that causes the retention of a pseudo-exon.
«We leveraged the extreme traits in different species to
uncover noncoding regions in the human genome that likely have important roles in shaping health and disease,» said Elliott Ferris, first author on the paper and a bioinformatician and computer programmer in Gregg's lab.
Moore's lab researches the mechanisms that underlie imbalances in metabolism as it relates to atherosclerosis and obesity,
including noncoding RNA's regulating role in cholesterol metabolism and inflammation.
Already, researchers have
linked noncoding DNA to Crohn disease, heart disease, multiple sclerosis, and other disorders, suggesting that enhancers could be targets for medical manipulation.
WGS is attractive here, because it enables you to look
at noncoding and structural variants in linkage regions, rather than taking a gene - centric approach.
Whole - genome sequencing identifies
inherited noncoding variants in families affected by autism spectrum disorder.
Small
noncoding microRNAs (miRNAs) have been found in such abundance that they have been christened the «dark matter» of the cell, a view reinforced by an analysis of the small RNAs found in Arabidopsis (pp. 1567 and 1525).
Regulatory proteins, RNA,
noncoding bits of DNA, even chemical and structural alterations of the genome itself control how, where, and when genes are expressed.
«By identifying this major regulator of
noncoding editing in C. elegans we can now focus on dissecting the regulatory mechanism and determining the conservation of this regulatory protein in human cells.»
«Molecular defenses spearheaded by small RNAs called piRNAs (my
favorite noncoding RNAs) can keep transposons from jumping,» Saey says.
The primate -
specific noncoding RNA HPAT5 regulates pluripotency during human preimplantation development and nuclear reprogramming.
Single - stranded,
noncoding micro-ribonucleic acids (microRNAs), consisting of 18 - 23 nucleotides, play a key role in regulating gene expression.
One gene set that stood out involved components of the spliceosome, the molecular complex that helps prepare messenger RNA (mRNA) transcripts for protein production by
removing noncoding segments called introns.
In addition to the 1,274 genes that take part in coding proteins, they also identified 375
novel noncoding gene families on the bovine Y chromosome, which are predominantly expressed in different stages of the testis.
They extracted and amplified DNA from these samples, concentrating on microsatellites,
repetitive noncoding DNA sections that rapidly accumulate genetic changes.
MicroRNAs are
short noncoding RNAs that play critical roles in regulating gene expression in normal physiology and disease.
Studies of the host response to viral infection have historically focused on protein - coding genes, thus our understanding of how the non-protein-coding transcriptome, including both viral - and host -
derived noncoding RNAs, impacts host - virus interactions is limited.