Sentences with phrase «noncoding sequences»

To provide a molecular basis for understanding N - CAM function, the complete amino acid sequences of the three major polypeptides of N - CAM and most of the noncoding sequences of their messenger RNA's were determined from the analysis of complementary DNA clones and were verified by amino acid sequences of selected CNBr fragments and proteolytic fragments.

«The elephant results revealed noncoding sequences in the human genome that we predict may control gene activity and reduce the formation of mutations and cancer.»

Many are probably simply the consequence of 6 million years of genetic drift, with little effect on body or behavior, whereas other small changes — perhaps in regulatory, noncoding sequences — may have dramatic consequences.

Not exact matches

Whole - genome sequencing identifies inherited noncoding variants in families affected by autism spectrum disorder.

Assessing the influence of individual DNA base changes is particularly difficult in noncoding regions, so they instead identified bigger alterations, so - called structural variants, in which large sequences of DNA are inverted, duplicated, or deleted.

They reveal 341 fixed substitutions (35 nonsynonymous, 173 synonymous, and 133 noncoding) between the 2014 EBOV and all previously published EBOV sequences, with an additional 55 single - nucleotide polymorphisms (SNPs; 15 nonsynonymous, 25 synonymous, and 15 noncoding), fixed within individual patients, within the West African outbreak.

Deep - sequence coverage allowed identification of 263 iSNVs (73 nonsynonymous, 108 synonymous, 70 noncoding, and 12 frameshift) in the Sierra Leone patients (6).

Noncoding DNA includes stretches called enhancers, short sequences that help control a target gene.

To their surprise and dismay, the genome turned out to contain long stretches of noncoding and repetitive DNA, which made it difficult to piece together the short reads produced by the sequencing machine.

The differences between coding versus noncoding trees were not solely due to shorter sequence length of the coding data, because the full coding data set (13.3 million bp for c123) produced a tree with fully supported (100 % BS) relationships that were incongruent with those fully supported in the intron (19.3 million bp), TENT (37.4 million bp without the third codon position), and WGT (322.1 million bp)(Figs. 2 and 5B, and table S3).

Extensive research has already examined the function of microRNAs, a category of small evolutionarily conserved noncoding RNAs about 22 to 24 nucleotides in length that target protein - coding genes in a sequence - specific manner.

But full sequencing will soon be just as cheap as exome sequencing and will catch disease mutations in noncoding regions as well, Gibbs notes.

Using a specific work flow, they assessed both the coding and noncoding regions of the human genome, including the evaluation of highly polymorphic SNPs, structural and copy number variations, as well as 69 control genomes sequenced by the same procedures.

And let's face it, exome sequencing lets us conveniently avoid some of the most challenging aspects of human genomics, like detecting complex rearrangements (SVs) and interpreting noncoding regulatory variants.

The Sequencing and Noncoding RNA Program is one of an MDA institution supported shared genomic service resources providing not only instrumentation but also expertise in applications of next generation sequencing and microarray services to invSequencing and Noncoding RNA Program is one of an MDA institution supported shared genomic service resources providing not only instrumentation but also expertise in applications of next generation sequencing and microarray services to invsequencing and microarray services to investigators

As our understanding of the noncoding portion of the genome improves, it will become even more apparent that whole - genome sequencing (and not exome sequencing) will be required to characterize the full extent of phenotypically - relevant genetic variation in humans.

Ironically, another reason people like exome sequencing is because of the limited scope: it essentially doesn't interrogate the regions (noncoding) and variant types (SVs) that are more difficult to interpret.

Filed Under: Cancer Genomics, Uncategorized Tagged With: cancer, DNA, genetics, genome, genomics, noncoding, research, selection, sequencing, snps, splicing, TCGA

In it, the authors review bioinformatics approaches for evaluating single - base substitutions in both coding and noncoding regions, with an emphasis on large cancer resequencing efforts including the Tumor Sequencing Project, the Cancer Genome Atlas (TCGA), and the AML1 cancer genome.

Genome editing technology enables precise modification of individual protein coding genes, as well as noncoding regulatory sequences, enabling the elucidation of functional effects in human disease relevant cellular systems.

With the sequencing and annotation of genomes and transcriptomes of several eukaryotes, the importance of noncoding RNA (ncRNA)- RNA molecules that are not translated to protein products - has become more evident.