To accomplish this, we used homologous recombination to ablate
nonmuscle myosin heavy chain (NMHC) II - B by inserting cDNA encoding green fluorescent protein (GFP)- NMHC II - A into the first coding exon of the Myh10 gene, thereby placing GFP - NMHC II - A under control of the endogenous II - B promoter.
The purpose of these studies was to learn whether one isoform of
nonmuscle myosin II, specifically
nonmuscle myosin II - A, could functionally replace a second one,
nonmuscle myosin II - B, in mice.