Sentences with phrase «normal gene expression»

In addition, it targets the cysteine in DNA methyltransferase enzymes, which play a role (DNA methylation) in normal gene expression.

Many patients suffering from Rare Disease harbour genomic variants (sequence variants or copy number variants) that by disrupting normal gene expression lead to disease.

Specifically, use of post-mortem material is crucial for studying the patterns of normal gene expression underlying tissue specificity within individuals, as sampling such tissues from living individuals would be impossible.

«Instead, after a few population doublings they go back to the normal gene expression pattern and rapidly reacquired drug sensitivity.»

«A totally normal - looking worm with a normal gene expression and stem cell distribution can in fact be harbouring a [body plan] that's quite different,» says Levin.

Not exact matches

The study's 31 samples of dog tumors was compared to 40 normal canine tissues samples as a way of estimating the variance in gene expression.

«Our work suggests that fine - tuning messenger stability is an important mechanism orchestrating gene expression changes during normal brain development.»

Using RNA sequencing, the researchers found multiple genes whose abnormal expression could lead to the high rate of death for cloned embryos, including failure to implant in the uterus and failure to develop a normal placenta.

Four days later, the livers of the non-supressed mice had readjusted to a normal daily rhythm, as revealed by the daily rise and fall of liver - gene expression.

Finally, the team identified a network of genes in these regions that exhibit changes in expression when the normal boost from serotonin is lacking, and these changes depended on how long the drug was given.

The researchers demonstrated that blocking the PGD enzyme genetically or with a pharmacologic inhibitor reversed the epigenetic reprogramming and malignant gene expression changes detected in distant metastases, and also strongly inhibited their tumor - forming capacity, with no effect on normal cells or peritoneal pancreatic cancer controls.

Cartilage formation (chondrogenesis) requires the normal function of Sox9, a protein that regulates the expression of chondrogenesis - related genes.

Their results showed that both forms of RP had clear differences in gene expression compared to normal retinas.

Of particular interest are the emerging techniques for genomics and proteomics, which allow profiles of gene expression and protein synthesis to be produced and comparisons to be made between normal and abnormal cells, as well as between cells before and after exposure to medicines or toxic chemicals.

In cells grown on flat culture dishes, the expression of thousands of genes didn't match up with their normal patterns, explaining why the cells from those dishes had been unable to generate new hair follicles.

To validate their findings, the scientists injected the novel nanoparticles into pancreatic tumor - bearing mice and observed that by balancing these two targets — bringing them to a normal level by increasing their expression or blocking the gene responsible for their expression — they significantly prolonged the survival of the mice.

When Bordenstein and Brucker compared gene expression patterns in normal and microbe - free offspring, they found significant changes in many of the genes involved in the immune response (Science, doi.org/m87).

This same feature, tumor heterogeneity, may reduce the ability to identify critical gene expression changes when comparing mean gene expression in adjacent tumor and normal tissues, as tumors of the same type may have different sets of genes differentially expressed.

By comparing the gene expression patterns of normal beta cells and insulin - producing cells derived from alpha cells, the researchers confirmed nearly complete cellular reprogramming.

Across all four cancer types, the new method of selecting candidate genes based on inter-tumor variation in gene expression outperformed the other methods, including the standard method of comparing mean expression in adjacent normal and tumor tissues.

Indeed, the team identified high variation in adjacent «normal» tissue samples, which are typically used as control samples for comparison in analyses based on mean gene expression.

In the new study, Burrows and colleagues focused on the protein HIC1, or «Hypermethylated in cancer 1, «so named because it was first identified in cancer cells; however, it also helps regulate gene expression in normal cells.

The results of the study challenge the model that comparing mean gene expression in adjacent normal and cancer tissues is the best approach to identifying cancer - associated genes.

Typical microarray - based gene expression analyses compare gene expression in adjacent normal and cancerous tissues.

The goal of the NIH program, as described on its website, is «to understand the principles behind the three - dimensional organization of the nucleus in space and time (the fourth dimension), the role nuclear organization plays in gene expression and cellular function, and how changes in the nuclear organization affect normal development as well as various diseases.»

On the flip side, said Tran, PGC1 alpha transgenic mice, which had increased expression of these genes, were particularly resistant to various stressors and recovered from injury much more than quickly than animals with normal regenerative abilities.

The alteration is predicted to result in the production of a shortened ETV6 protein that can not fulfill its normal function of binding to DNA and regulating the expression of other genes.

The aim now is to identify the molecules that regulate expression of this gene upstream, and those that interact with the enzyme, to understand their modes of action and determine how normal MOCOS expression could be restored..

When the researchers eliminated the expression of this gene in the CAFs, these cells behaved like normal fibroblasts, as they lost the ability to help the tumour cells to progress.

But new research presented today at the American Physiological Society (APS) annual meeting at Experimental Biology 2017 in Chicago suggests that normal levels of vitamin A within a high - fat diet can negatively affect expression of liver genes associated with glucose and fat metabolism.

Working with Bhaduri, who has a background in statistics and bioinformatics, Pollen and Nowakowski began exploring how specific classes of neurons and stem cells in the developing brain contribute to normal brain growth as well as to neurodevelopmental disease, and have begun to build a comprehensive, open - source atlas of gene expression across the developing brain, which they hope will serve as a resource for other scientists.

They compared space - flown endothelial cells to endothelial cells cultured under normal gravity, looking for differences in gene expression and / or in the profile of secreted proteins.

The researchers discovered that the activity of this electric signal is necessary in different aspects of normal regeneration, namely for the expression of crucial genes, formation of a mass of cells capable of growth and regenerate, and proper tissue innervation.

Supplementing B vitamins into the insect's diet or reestablishing the symbiotic partnership restores normal expression patterns of those genes.

The gene expression in the SCN, however, looked normal.

When studying how normal cells change into cancer cells, dos Santos and other cancer researchers pay close attention to gene expression.

«We observed small but significant changes in the gene expressions between normal and diabetic corneas,» said Mehrnoosh Saghizadeh Ghiam, PhD, assistant professor of biomedical sciences and neurosurgery, a researcher in the Regenerative Medicine Institute Eye Program and the lead author of the study published in the journal PLOS ONE.

With these tools, Verma is revealing how the aberrant expression of normal cellular genes can causes tumors.

Gene expression patterns in normal adult or the developing retina have been reported for mouse [18 — 23], aged and normal adult human [24 — 26], canine [27], and rabbit [28].

The normal tissue atlas now provide a distribution map of both protein and gene expression.

However, this cellular intervention alters the normal expression of hypophosphorylated retinoblastoma (RB) protein needed for the expression of genes involved in cell functions [3] and, therefore, results in an abnormal cell.

A detailed comparison of gene expression signatures between ETP ALL tumors and and normal human hematopoietic progenitor cells revealed a somewhat surprising finding: ETP - ALL expression patterns were less consistent with early T - cell precursors, as might have been expected, but more similar to the expression profile of normal hematopoietic stem cells and granulocyte macrophage precursors.

It's possible that inherited regions of CNV with strong influence on gene expression are targeted for amplification / deletion by cancer cells, which might obscure their effect in an otherwise normal cell.

Among the study findings, IR mutations alter expression of many genes both in fibroblasts and iPSCs compared to normal cells, «but the impact is very much dependent on the cell type,» says Kahn.

Part of the normal variation, which is equally present between blastocysts of both groups, can be observed in the gene expression of levels mtDNA encoded OXPHOS proteins.

Gene expression was compared between corals treated with elevated and normal calcium concentrations.

Changes in the manipulation medium, as well as a move from late to early stages after first appearance of pronuclei, produced blastocysts which were statistically indistinguishable from those produced in normal IVF by measures of morphology, ploidy and gene expression.

«This tells us that vitamin A can enter at some point in the pathway and restore normal expression of the genes such as lefty1 involved in the process,» said Izpisúa Belmonte.

Now, they have investigated gene expression in low - grade dysplastic lesions and normal stomach tissue from mice and in gastric cancer and normal stomach tissue from humans.

Gene Expression Differences in Normal Esophageal Mucosa Associated with Regression and Progression of Mild and Moderate Squamous Dysplasia in a High - Risk Chinese Population