Sentences with phrase «normal goat serum»

The cells were 4 % PFA fixed (10 min) and then incubated in 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1h to permeabilise the cells and block non-specific protein - protein interactions.

The sections were blocked in 1 % milk, 10 % normal goat serum (Vector Laboratories), and 0.2 % gelatin prior to overnight incubation in primary antibody.

The cells were fixed with 100 % methanol for 5 minutes, permeabilized with 0.1 % Triton X-100 for 5 minutes and then blocked with 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1 hour.

After blocking for 1 hour with 10 % normal goat serum (NGS), the slides were incubated with 200 μl primary antibody, either a polyclonal rabbit anti-Borrelia (Accurate chemicals, Westbury, NY) at 1:200 or the anti-OspA monoclonal, CB10, with a 1:30 dilution of hybridoma supernatant for 1 hour at room temperature (RT).

The cells were fixed with 4 % formaldehyde (10 min), permeabilized in 0.1 % Triton X-100 for 5 minutes and then blocked in 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1h.

Sections were incubated in 0.3 % hydrogen peroxide in PBS for 20 min, washed in PBS - T (0.01 M phosphate - buffered saline, 0.2 % Triton X-100) and blocked 1 h with 10 % normal goat serum (NGS) in PBS - T.

Isolated retinas were blocked and permeabilized with retina blocking buffer (10 % normal goat serum, Dako, #X0907, in 0.5 % Triton - X 100 and 1 % BSA in PBS) for 1 h at RT..

The cells were fixed with 4 % formaldehyde (10 min) and then blocked in 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1h.

The cells were fixed with 4 % formaldehyde (10 min), permeabilized with 0.1 % Triton X-100 for 5 minutes and then blocked in 1 % BSA / 10 % normal goat serum / 0.3 M glycine in 0.1 % PBS - Tween for 1h.

Not exact matches

Coverslips plated with acutely dissociated hNPCctx - GDNF or hNPCctx were fixed with 4 % paraformaldehyde, washed with PBS, blocked in 5 % normal donkey serum and 0.1 % Triton X-100, and incubated with goat anti-GDNF (1 ∶ 100; R&D Systems) primary antibody followed by donkey anti-goat Cy3 - conjugated secondary antibody (1 ∶ 1000; Jackson IR).

The concentration of in - situ antibody - AChR complexes was determined by precipitation with goat anti-rat serum in the presence of normal rat serum.