"Nuclear staining" refers to a process where a substance called a dye is used to color or mark the nucleus, which is the central part of a cell. This staining technique helps scientists or doctors observe and study the cell's nucleus more easily under a microscope.
Full definition
To further confirm this observation, the intensity
of nuclear staining and the percentage of nuclear positive cells obtained from immunohistochemistry analysis of 17 normal epidermis and 34 SCC specimens were analyzed statistically.
In addition to the cytoplasmic staining for p - p70S6K, we also detected
nuclear staining in a significant proportion of ALK + ALCL tumors.
Recommended incubation for nuclear counterstains: RT 5 min incubation with 0.1 — 1 μg /
mL nuclear stain dye and further extensive PBS washing (Figure 3).
The intensity
of nuclear staining was divided into four levels: negative, slight, moderate, and strong.
The different organelle probes are displayed as different channels in the multicolor images; the HPA antibody staining is shown in green,
nuclear stain in blue, microtubules in red and ER in yellow.
Switching
the nuclear stain from DAPI (4», 6 - diamidino -2-phenylindole; emission in the blue region) to DRAQ5 (deep red anthraquinone 5, which has red emission) frees the violet — blue region of the spectrum to accommodate the two Brilliant Violet dyes.
DDX3 was strongly immunolocalized in the nucleus and less intensively in the cytosol of squamous cells in normal skin, but
the nuclear staining was lost or dramatically decreased in cutaneous SCC.
As shown in Fig. 6B, both
the nuclear staining intensity of DDX3 and the percentage of cells positive in DDX3 nuclear staining were much higher in normal epidermis as compared with neoplastic squamous cells (normal versus SCC, 94 % versus 18 % cases for strong and moderate grade intensity; 94 % versus 12 % cases with > 50 % DDX3 nuclear staining positive cells; P < 0.001).
The intensity of DDX3
nuclear staining and the percentage of DDX3 nuclear staining positive cells in normal skin and cutaneous SCC tissues are summarized.
Cells were stained with anti-human IgG (green) and DAPI
nuclear stain (blue).