We also discovered that
this nuclease activity varies greatly depending on the cell type.Until now, the fate of exogenous DNA introduced into a cell was treated as a black box.
Through careful mapping of the ribose requirements of Cas9, we develop hybrid versions possessing minimal RNA residues, which are sufficient to direct specific
nuclease activity in vitro and in vivo with reduced off - target activity.
In a paper appearing online today in the journal Nature, the team reports the results of biochemical assays which demonstrate that mZuc has no phospholipase activity and that it does in fact possess
nuclease activity.
The point mutation was induced by forming a synthetic complex through removal of
nuclease activity from the CRISPR system — a technique using artificial nuclease — and addition of deaminase, a deaminizing (base - modifying) enzyme, and then expressing it in yeasts and mammalian cells.
Not exact matches
With this information, they turned to a cell culture system to see if any Cpf1
nucleases would exhibit in vivo
activity in mammalian cells.
The enzyme
activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4 — Not
nuclease module