Sentences with phrase «nuclease assay»

Next, we examined the frequency of CXCR4 DNA disruption over time with the surveyor nuclease assay.

In the presence of Bk132, treatment with X4 - ZFNs conferred protection at 14 d.p.i (p =.05); however, this protection wanes by 34 d.p.i. (p =.88)(B) Cxcr4 disruption frequency was assessed by the surveyor nuclease assay in both peripheral blood (p <.001) and spleen (p <.001).

Cell growth was monitored every 48 hours post-stimulation for approximately two weeks and the efficiency of CXCR4 disruption was assessed at day five post-transduction by both the Surveyor nuclease assay and by deep - sequencing of the CXCR4 target site.

For humanized mice samples, whole genome amplification using the REPLI - g Mini Kit (Qiagen) was conducted prior to the surveyor nuclease assay due to limiting cell numbers.

Together the stable disruption of CXCR4 as determined by both the surveyor nuclease assay and flow cytometry suggests that CXCR4 disruption did not negatively impact cell viability or growth in humanized NSG mice over a two - month period.

Development and evaluation of a fluorogenic 5 ′ nuclease assay to detect and differentiate between Ebola virus subtypes Zaire and Sudan

Not exact matches

The treated PCR products were diluted with 50 uL of nuclease - free water before Quant - IT ™ PicoGreen ® dsDNA Assay Kit quantification (http://www.lifetechnologies.com) on a plate reader.

In a paper appearing online today in the journal Nature, the team reports the results of biochemical assays which demonstrate that mZuc has no phospholipase activity and that it does in fact possess nuclease activity.

Each assay (15 µL total volue) contained nuclease free water (5 - 10 µL), cDNA template (2 ng / µL), and gene - specific primers (4.5 pm / µL).