Not exact matches
«If the probe gets
cleaved by serum
nucleases, then our probe would be lit up all over the bloodstream.
At sites complementary to the crRNA - guide sequence, the Cas9 HNH
nuclease domain
cleaves the complementary strand, whereas the Cas9 RuvC - like domain
cleaves the noncomplementary strand.
In tests,
nucleases (or enzymes) produced by the staph bacteria
cleave the particles, like a warrior wielding a sword.
These two
nucleases, AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both
cleave in a staggered pattern similar to FnCpf1.
It was suspected, but not known for certain, that fly as well as mouse Zucchini proteins were
nucleases — enzymes that specialize in
cleaving bonds between the nucleotide subunits of nucleic acids (DNA and RNA).