Sentences with phrase «nuclease contains»
The Cas9 nuclease contains two active sites.
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Compounding this risk, the liver cells that now contain the DNA for ZFNs will keep making the nucleases for perhaps years, even though they are no longer needed to guide the new gene to its spot in the genome.
Engineered CRISPR systems for gene editing now contain two main components, a single guide RNA or sgRNA and Cas - 9 nuclease.
Each assay (15 µL total volue) contained nuclease free water (5 - 10 µL), cDNA template (2 ng / µL), and gene - specific primers (4.5 pm / µL).