Sentences with phrase «nuclease free»
Each assay (15 µL total volue) contained nuclease free water (5 - 10 µL), cDNA template (2 ng / µL), and gene - specific primers (4.5 pm / µL).
http://www.thecmmr.org/ Not exact matches
Samples of cDNA were diluted 1:25 in nuclease - free water (Qiagen Inc.).
The treated PCR products were diluted with 50 uL of nuclease - free water before Quant - IT ™ PicoGreen ® dsDNA Assay Kit quantification (http://www.lifetechnologies.com) on a plate reader.
The 50 uL PCR reaction consisted of the following mixture: 10 uL 5X LongAmp Taq Reaction Buffer, 1.5 uL of dNTPs (1 mM), 4 uL of each primer pairs (10 uM each, see below for details), 2 uL of template DNA, 2 uL of LongAmp Hot Start Taq DNA polymerase (2500 U / mL) and 30.5 uL of nuclease - free water.