Fluorescence images were used to quantify the following muscle morphological parameters: total fiber number, single - fiber CSA, total nuclei, the percentage of centrally
nucleated fibers (CNFs), and the nuclei / single - fiber area ratio.
In addition, the percentage of centrally
nucleated muscle
fibers was also significantly increased in both EDL and TA muscles from IL - 15Rα — KO compared with control mice (Figure 6, C and G).