Sentences with word «nucleofection»

Following introduction into human CD4 + T cells [23] or hematopoietic stem cells [28] via an adenovirus vector or DNA nucleofection, respectively, the ccr5 gene was efficiently and specifically disrupted.
We prefer to culture our cells on mouse embryo fibroblasts (MEFs) but occasionally grow the cells feeder free for particular applications, such as nucleofection, viral transduction or karyotyping.
For every one genetically altered cell generated using the chemical method, the new growth factor / nucleofection method produces between 10 and 100 successfully modified cells, UCI scientists estimate.
The UCI scientists — Donovan, Lock and Kristi Hohenstein, a stem cell scientist in Donovan's lab — used those growth factors in the current study to keep cells alive, then they used a technique called nucleofection to insert DNA into the cells.
I prefer to culture my hPSCs with mouse embryonic fibroblasts (MEFs) but there are occasions when feeder free growth is required (e.g., nucleofection, viral transduction or karyotyping to name a few).
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