Sentences with word «nucleolus»

They used the stage of meiosis in Xenopus oocytes where you find thousands of nucleoli, and everything in the field pointed to the fact they contained amplified genes for ribosomal RNA, and indeed that turned out to be the case.

E, iPSCs have a large nucleus - to - cytoplasm ratio and prominent nucleoli when compared to original NSCs (F).

Mice's DNA were modified in a manner that only defective nucleoli could be formed by the cells producing dopamine.

Related sites Lamond lab nucleolus database Biochemistry of the cell nucleus Tom Misteli's home page

The next step was to apply the method to chromosomes themselves rather than amplified nucleoli, and I was part of that effort.

In interphase, forms fiber - like structures in fibrillarin - deficient regions surrounding nucleoli (PubMed: 2674163, PubMed: 8799815).

Steinman and Cohen noted in their seminal 1973 paper in the Journal of Experimental Medicine: «The nucleus is large, retractile, contorted in shape, and contains small nucleoli (usually two).

A still from a movie showing nucleoli quickly falling and coalescing into larger droplets at the bottom of the nucleus when actin is disrupted.

Tumor cells exhibit nuclear enlargement, opened chromatin and multiple nucleoli.

Most nucleoli were in fact discovered to be defective inside these cells.

This discovery led the researchers to take a look at if disrupted nucleoli can really result in Parkinson's - like symptoms or if this was just an incidental discovery.

As seen in Figure 2, the abnormal BM cells were found to exhibit eccentric, large, round - to - oval / convoluted nuclei with mostly fine chromatin and prominent nucleoli and moderate - to - abundant gray cytoplasm with several cells exhibiting multiple diminutive cytoplasmic vacuoles.

Oxidative stress in cells is apparently caused by defective nucleoli.

That was surprising, he explains, because «fibrillarin resides deep within the nucleolus of the host cell... [where it] methylates ribosomal RNA molecules, which then go on to form ribosomes,» but its full function may not be completely understood.

The latest such roster — 271 proteins identified in a compartment of the cell nucleus called the nucleolus — is a first step toward fully deciphering this organelle, a critical element in the construction of ribosomes, which build proteins.

To tally the proteins, the largest ever reported for a single organelle, cell biologists Angus Lamond of Scotland's University of Dundee, Matthias Mann of the University of Southern Denmark in Odense, and their colleagues first separated the nucleolus from its gelatinous home in the nucleus.

But everyone cautions that the list may include contaminant proteins because the nucleolus is difficult to separate from the nucleus.

As Lamond and his colleagues report in the 8 January issue of Current Biology, 191 of the proteins are known characters, and over half of them were previously identified with the nucleolus.

This set includes various ribosome builders, as well as other proteins that process and transcribe ribosomal RNA, a necessary step before the ribosomes can be assembled and pushed out of the nucleolus.

«The actual overall complexity of the nucleolus was somewhat surprising,» says Lamond, who suspects that it's far more than a ribosome factory.

The male and female pronuclei (large spheres) are melding to form a full set of chromosomes, and the alignment of the nucleoli (small spheres) is normal.

Finally, they demonstrated that the dissolved nucleoli could reassemble in the test tube, if supplied with the necessary raw materials, showing that FRGY2 doesn't just tear the nucleolus apart.

Understanding how the cell dismantles the nucleolus during cloning could help researchers understand how an adult nucleus becomes «reprogrammed» to develop as an embryo, Kikyo says.

Somehow, the protein carefully disassembles the nucleolus, leaving its core structure intact and able to reassemble itself.

In test tube experiments, the researchers showed that the FRGY2a protein alone could cause the nucleolus to disassemble.

When the researchers then added the active region of FRGY2a to live cells, the nucleoli dispersed as expected.

Some scientists suspected that the nucleolus — a small body inside the nucleus that puts together the cell's protein - producing machinery — could play a key role in this remarkable change.

Previous studies found that the nucleolus disappears soon after the adult nucleus is added to a frog egg, only to reappear later.

Products in the CytoPainter brand allow staining of acting filaments, mitochondria, lysosomes, endoplasmic reticulum, golgi apparatus and nucleoli.

The iPS cells displayed typical ES cell - like morphology, including a high nucleus - to - cytoplasm ratio, prominent nucleoli and clear boundaries among cells.

The iPSC colonies were morphologically indistinguishable from hESCs, forming tight colonies of cells with a large nucleus to cytoplasm ratio and prominent nucleoli (Fig. 2E), and they did not display the NSCs» original cell morphology (Fig. 2F).

Nucleus, nucleolus.

The same cells, stained to reveal their nucleoli and endoplasmic reticulum.

In addition, the monopolin complex maintains the integrity and silencing of ribosomal DNA (rDNA) repeats in the nucleolus.

With Cell Painting, a technique developed at the Broad, researchers tag eight cellular components and organelles (actin, cytoplasmic RNA, endoplasmic reticulum, Golgi apparatus, mitochondria, nucleus, nucleolus, and the plasma membrane) with fluorescent dyes.

Remarkably, other organelles, like for example the nucleolus, are not surrounded by membranes.

Figure 1: Live - cell imaging of Ki - 67 (green) during the cell cycle reveals its re-localisation from nucleoli to the surface of mitotic chromosomes (magenta).

In prokaryotes, rRNA is made in the cytoplasm; in eukaryotes, rRNA is made in the nucleolus.

Researchers have explored if the nucleoli's demise also plays a part in Parkinson's, also referred to as «shaking palsy».

Defective nucleoli (small nuclei) have been implicated in a number of rare hereditary conditions, as well as in neurodegenerative diseases like Huntington's and Alzheimer's.

The researchers aren't however sure if the nucleoli damage is really the only reason for this neurodegeneration.

Furthermore, there is evidence suggesting an association of replication stress on rDNA loci with the aging of hematopoietic stem cells, adding more evidence to the general function of the nucleolus in genome integrity and aging.

Multiple studies have reported a link between rDNA stability and cellular aging, as well as the association of proteins involved in genome integrity transiting the nucleolus.

VERTEBRAE WITH THE NUCLEOSUS PULPOSUS - Shows the actual disc between the vertebrae with the central location of the nucleolus pulposus.

Histologic findings show mature, neuronal - like cells with multiple processes, a Central nucleus and a nucleolus.

It shows Merz's keen grasp of geometric minimalism, architectural awareness, and a sense that the nucleolus of our lives are the people who are closest to us.