Mechanistic investigations revealed that RASSF1A blocked tumor growth by stimulating cofilin / PP2A - mediated dephosphorylation of the guanine
nucleotide exchange factor GEF - H1, thereby stimulating its ability to activate the antimetastatic small GTPase RhoB.
In the JBC article, Lipschutz and his MUSC coauthors go a step further — showing in cell culture and a zebrafish model that depletion of Tuba, a guanine
nucleotide exchange factor required for Cdc42 activation, also disrupts renal ciliogenesis.
Depletion of dynamin - binding protein or Tuba, a guanine
nucleotide exchange factor, disrupted renal ciliogenesis in cell culture and led to abnormal kidney morphology in a Tuba knockdown zebrafish model of PKD.