At the highest level of resolution, the user can inspect
the nucleotide sequence of individual genes.
To obtain cDNA fragments of the kakusei homolog in Japanese honeybee, PCR was performed using a set of primers designed based on
the nucleotide sequence of the European honeybee kakusei, 5 ′ - GGGGAAGCCAGGAGCCGCGGGTTTACAT - 3 ′ and 5 ′ - AGGCAACAGCACACCATGGGCCTTGGAT - 3 ′, with Ex Taq (Takara, Tokyo, Japan).
The isolation and
nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family.
Nucleotide sequence of first 1000 bases of HIV - 1 strain HXB2 provirus.
And since we know
the nucleotide sequence of every known microRNA, all that is required is to prepare an anti-miR with a sequence that is exactly the opposite of the miR's,» he said.
Zhang compared the DNA
nucleotide sequence of the human version of ASPM to that of two of our great ape cousins, the chimpanzee and the orangutan, as well to more distantly related animals such as rhesus monkeys, seals, dogs, and hamsters.
We elucidated
the nucleotide sequence of a gene, and this was published in 1972.
Fiers investigated
the nucleotide sequence of the hemagglutinin gene of this duck virus and found that it was indeed very closely related to the strain that started the 1968 Hong Kong outbreak.
In 1972 he and his team were the first to publish
the nucleotide sequence of a complete gene.
There is no such «direct» evolution: animals, bacteria, and algae have a common ancestor from which they have diverged, as can be shown by aligning and comparing amino acid sequences of proteins and
nucleotide sequences of homologous ribosomal RNA molecules that are found in both bacteria and vertebrates.
Working with French composer Richard Krüll, the pair turned the complete
nucleotide sequences of several microbe genes into compositions based on DNA bases: A (adenosine), C (cytosine), G (guanine), and Thymine (which they have translated to «Re,» or D).
Comparison of the structure and
nucleotide sequences of Acks and kakusei cDNAs revealed that the nucleotide sequences corresponding to +1946 b to +5175 b of Acks cDNA was equivalent to the kakusei cDNA corresponding to the neural activity - inducible transcript (Figure 1A).
The nucleotide sequences of Acks cDNA of approximately 7.1 kb in length shared approximately 85 % sequence identity with the European honeybee kakusei cDNA, and had additional unique sequences of approximately 700b at its 3 ′ end.
The nucleotide sequences of the 16S — 23S rDNA spacers for the B. burgdorferi isolates analyzed in figure 1 were deposited in GenBank (accession numbers, AF139507 — AF139517).
Not exact matches
DNA, made up
of four
nucleotide molecules in a
sequence, is a code that can be edited and written — not unlike software.
The specific
sequence of nucleotides determines the various kinds
of life that appear in evolution.
In the words
of chemist - turned - philosopher Michael Polanyi, the
sequence of nucleotides is «extraneous to» the physical and chemical properties within the molecule» which is to say, the
sequence is not determined by inherent physical - chemical forces.
To string millions and billions
of nucleotides into a
sequence that makes living beings able to live and reproduced, and then say, «Well TIME (little god), made that possible through many, many small changes over time», is also a lot
of faith.
The kit also preserves the single -
nucleotide variants (SNVs) and SNV frequencies across different
sequencing technologies and allows the amplification
of cell - free DNA to levels that permit
sequencing.
Every tissue in an organism contains a variety
of messenger RNA molecules, single - stranded
nucleotide sequences bearing instructions for the making
of specific proteins.
The
nucleotide in M1 RNA, residue C92, which participates in a crosslink with the substrate was deleted and the resulting mutant M1 RNA was shown to cleave substrates lacking the 3» terminal CCAUCA
sequence at sites several
nucleotides away from the normal site
of cleavage.
The Human Genome Project, which
sequenced the 3 billion pairs
of nucleotide bases in human DNA, was a piece
of cake in comparison: Epigenetic markers and patterns are different in every tissue type in the human body and also change over time.
They use one
of several proprietary methods to determine the
sequence of nucleotide «letters» (A, C, G and T) that encode genetic information.
In the new study, the researchers
sequenced the entire genome
of mice that had undergone CRISPR gene editing in the team's previous study and looked for all mutations, including those that only altered a single
nucleotide.
A catalytic RNA (ribozyme) derived from an intervening
sequence (IVS) RNA
of Tetrahymena thermophila will catalyze an RNA polymerization reaction in which pentacytidylic acid (C5) is extended by the successive addition
of mononucleotides derived from a guanylyl -(3», 5»)-
nucleotide (GpN).
Which genes are activated and which are turned off is in part determined by a second tier
of information which is superimposed on the
nucleotide sequences that provide the blueprints for protein synthesis.
It is a term
of art in patent law that renders the claim «open - ended» so that it covers both the listed
nucleotide sequence and, more importantly, the listed
sequence in combination with additional
sequences.
A genome consists
of only four different
nucleotide bases, or DNA subunits, arranged in a particular
sequence.
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder that has been linked to toxic aggregates
of poly - Gly - Ala (poly - GA) peptides generated by aberrant translation
of an expanded
nucleotide repeat
sequence.
The Genographic Project: Testing My Maternal Ancestry Commercial testing companies do not need to test my whole genetic
sequence — all 3 billion
nucleotides, the «letters»
of the genetic code — to tap into my past.
That sort
of resolution should be good enough to determine the
sequence of all the
nucleotide bases in the human genetic code.
We present a draft
sequence of the Neandertal genome composed
of more than 4 billion
nucleotides from three individuals.
This database is produced at National Center for Biotechnology Information (NCBI) as part
of the International
Nucleotide Sequence Database Collaboration, or INSDC.
We have determined additional full - length
sequences from two clusters
of imported EBOV infections into Mali, and we show that the
nucleotide substitution rate (1.3 × 10 — 3 substitutions per site per year) is consistent with rates observed in Central African outbreaks.
Fully
sequenced genomes remain rare, so the bulk
of the analysis was done by looking at genetic markers known as single
nucleotide polymorphisms or SNPs.
It starts with a long DNA strand — called a scaffold — that has a precise
sequence of the four molecular units, or
nucleotides, dubbed A, C, G, and T, with which DNA spells out its genetic code.
In germline cells PIWI proteins silence the RNA from jumping genes by cutting them in
sequences of ~ 30
nucleotides that will become piRNAs.
In the 7 May 2010 issue
of Science, an international team
of researchers presents the draft
sequence of the Neandertal genome composed
of over 3 billion
nucleotides from three individuals.
DNA is made
of four substances — the
nucleotide bases adenine, guanine, thymine, and cytosine — that will combine only in specific configurations and
sequences.
Analysis
of partial
nucleotide sequences has enabled the team to refine our picture
of the evolutionary history
of cervids.
Bustamante and Poznik obtained highly accurate
sequencing results over a length
of about 10 megabases
of Y chromosome DNA (or 10 million
nucleotides) for each
of the 69 individuals.
«By analysing the first
nucleotides we are able to identify which
sequence of gene starts the transcription.
Their trouble with language had been caused by the change
of a single
nucleotide of DNA — just one letter in the genetic
sequence.
The blade, he found, is an enzyme that always cuts the viral DNA code where it finds a particular short
sequence of nucleotides.
Even if we assume that a trillion trillion trillion trillion unique
sequences 180
nucleotides long could function as RNA replicases, the probability
of hitting any one
of them in a single shot is still only about 1 in 1060.
The 5386
nucleotides of fC became the first complete DNA to be
sequenced (by Fred Sanger's laboratory in Cambridge in 1977) and Sinsheimer is justifiably proud at having put fC on the map.
Bartlett and Davidson devised a technique called Forensically Informative
Nucleotide Sequencing, or FINS, mainly for use in verifying that processed fish products are made
of the species they claim to be.
Normally, mutations in genetic
sequences that give rise to herbicide resistance happen at the scale
of a single
nucleotide.
A chromosome is, minimally, a very long, continuous piece
of DNA, which contains many genes, regulatory elements and other intervening
nucleotide sequences.
Once the appropriate
sequence of nucleotides is known, the aptamers can be easily generated.