CSCs were prepared from 231BrM and CN34BrM cells that were infected with lentivirus carrying with or without miR -7-2, and they were transplanted into
nude mice through intracardiac injection followed by monitoring metastatic tumor growth in the brain.
In these experiments, Berger and colleagues show that somatic PREX2 mutations identified
through whole - genome sequencing of human melanoma can contribute to enhanced lethality of tumor xenografts in
nude mice (Figure 3B, S6B, and S6C; Berger et al., 2012).