Sentences with phrase «number of cell cultures»

To answer these questions, the scientists went in a new direction: They performed infection trials in a large number of cell cultures at different temperature levels.

«In the future, this will move us towards a way to understand how to generate a cardiac muscle or other specific cell type without having to try an unwieldy number of cell culture conditions.»

Not exact matches

Embryos are different from mere cell cultures in a number of important ways.

The researchers then examined a number of DNA variants in the protein production - control region of the bellwether gene in cell cultures.

Fat cells cultured from the body mass index of a morbidly obese patient cause multiple myeloma cells to anchor to a much greater extent than normal cells and produce a significantly larger number of blood vessels to sustain the cancer cells.

Mackay - Sim's team has solved those problems by developing a method of removing the cells under a local anaesthetic and growing them in culture to produce larger numbers.

The latter can be primed in tissue culture by providing small numbers of dendritic cells.

They are also trying to culture the undifferentiated cells for longer before adding Sonic hedgehog, so that they can grow larger numbers of cells.

In addition to emphasizing the recruitment of graduate students, American universities have a number of on - campus service facilities such as modern research labs, well - stocked reagent rooms, and cell culture facilities.

An APC - mimetic scaffold that was engineered to activate a specific type of CAR - T cell was able to generate higher numbers of the modified T cells over longer periods of culture than analogously designed expansion beads, and the resulting cells were similarly effective in killing the lymphoma cells in the mice.

Prior research with cultured tissue had shown that a mix of chemicals could change bone marrow stem cells from mice to those resembling brain cells, but when a team led by neurologist Lorraine Iacovitti of Thomas Jefferson University in Philadelphia tried the same brew on human cells, the number altered was modest.

After collecting biopsies from banked human donor eyes, the team expanded the numbers of cells in a culture plate using human serum to nourish them.

Kass developed the midstream urine test (still used when you pee in a cup) and set a numerical cutoff for the number of bacteria in normal urine: not more than 100,000 colony - forming units (cell clusters on a culture dish) per milliliter of urine.

«Our group pioneered the development of cell culture technology for harvesting large numbers of stem cells from human bone marrow and human umbilical cord blood,» Dr. Yeh said, noting that stem cells from these two sources are abundant and can be guided into different types of cells using tissue engineering.

This distributed biological memory leverages the large number of cells in bacterial cultures and encodes information into their collective genomic DNA in the form of the fraction of cells that carry specific mutations.

Since Emiliania huxleyi cells divide about once a day at the laboratory, a large number of genetically identical starting cultures were obtained from the isolate.

Normal cells often have proved impractical because they can only divide a limited number of times in culture, and once returned to the body they're often too old to do much good.

One of the major impediments to obtaining a large number of endothelial cells from different tissues has been the inability to purify and propagate these cells in culture.

D, the same primary breast cancer cells infected with KLF4 or control lentivirus were cultured in low - attachment plates and the number of mammospheres was counted after 10 days.

Efforts to promote a selective increase in the number of endothelial cells present in primary cultures by using different media preparations (MCDB - 131, D - Valine, etc.) were largely unsuccessful.

Using cell culture assays and genetically modified mice, we identified a number of signaling proteins that participate in muscle plasticity.

The Krainer team, in collaboration with a team at Isis Pharmaceuticals, surveyed a large number of antisense oligonucleotides (ASOs) and found that some of these ASOs are able to correct the mRNA splicing defect in cultured cells from SMA patients.

Mouse embryonic stem cells stained for key pluripotency markersGRAZIANO MARTELLO Combining computer science algorithms with biological data has enabled researchers to create a model of the minimal number of factors necessary for mouse embryonic stem cells (ESCs) to self - renew in culture.

If the User requests isolated cells, Users will provide an account number for a charge - back of $ 100 for each hepatocyte / Kupffer cell isolation, to cover the cost of biochemicals, gradients and cell culture media required for isolation of purified cell populations.

NCI's efforts to develop new laboratory models of human cancer includes vastly increasing the number of human cancer cell lines (grown as two - dimensional and three - dimensional cultures) and patient - derived tumor xenografts.

The impaired ability of human corneal endothelial cells (HCECs) to divide, both in vivo and in culture, has been well documented in the scientific literature for a number of years [1 - 6].

Culture expansion of cells can take several weeks depending on the number of cells needed.

After addition of exogenous IL - 2, the levels of IFN - γ production in culture media of stimulated CD8 + T cells increased in all genotypes, again likely attributable to cell number (Fig. 3F).

A new study demonstrates how a three - dimensional culture substrate can help to maintain the number of primitive stem cells present during the expansion...

Bone marrow cells cultured with conditioned medium derived from ABL1 / ABL2 knockdown 1833 and SKBR3 breast cancer cells had decreased numbers of TRAP + cells compared to the control groups (Fig. 5, B and C, and fig.

Due to the large number of hES cells and human induced Pluripotent Stem (hiPS) cells needed by I - Stem's research groups, the development of large - scale culture systems for expansion and differentiation of those cells is critical.

Our goal is to implement the process transfert of a number of bench - scale manual cell culture protocols developed and optimized at I - Stem to an entirely automated cell culture platform which will enable more reproducible procedures and provide critical savings on technical time and costs.

This BaF3 phe116 almost completely fails to proliferate and activate STATs in response to IL - 9.15 However, upon prolonged culture with IL - 9, a small number of cells manage to survive and even proliferate, allowing an IL -9-dependent cell line (BaF3 phe116 / 9) to be selected.14 In contrast to parental BaF3 phe116 cells, those IL -9-selected cells could progress to autonomous cells (BaF3 Aut) after a second selection step in the absence of cytokine.

(A, B) Neurite length of neurons in E14 rat VM cultures transfected with control siRNA, Smad4 siRNA or Smad1 siRNA or Smad5 siRNA and a GFP - expressing plasmid before being cultured with or without 10 ng / ml GDF5 for 72 h (*** P < 0.001 compared with control; ANOVA with post-hoc Tukey's test; 40 cells for each group per experiment; number of repetitions (n) = 3 experiments).

In addition, work may eventually be allowed on a limited number of stem cell cultures imported from other countries.

Results: During in vitro culture period, the percentages and absolute numbers of T cells expressing the CARs containing a hinge domain continuously increased, mainly through the promotion of CD4 + CAR T cell expansion, regardless of the single - chain variable fragment (scFv).

And while the human and T cells they studied in the laboratory were not specifically skin T cells they were isolated from mouse cell culture and from human blood — the skin has a large share of T cells in humans, he says, approximately twice the number circulating in the blood.

We measured the expression of ALDH1 in cell lines following normoxic and hypoxic culture and, in support of the MFC and HFC data shown above, show increased ALDH1 - positive cell number in ER - α — positive lines and a contrasting decrease in ER - α — negative lines (Fig. 1D).

An equal number of MRL responder splenocytes were added and the cells cultured for 48 h before pulsing.

The low density culture protocol helps in generating larger number of CD133 + endothelial population from initially fewer CD133 + endothelial progenitor cells; EPCs (Figure S1).

Influence of semi-defined and defined culture media on the development, cell number and metabolism of bovine embryos.

One study on the culturing method involved in the production of the Japanese traditional food «Miso» concluded that the culturing process itself led to a lower number and growth rate of cancer cells.

In a study involving dietary ketosis via a low carbohydrate diet (less than 10 percent of total calories), compared to subjects on a 50 percent carbohydrate diet, the low - carbohydrate subjects demonstrated better performance on memory tests, with higher scores being correlated to higher serum KB levels.14 A study using cultured mouse hippocampal cells showed that addition of the KB β - hydroxybutyrate (β - OHB) to cells exposed to Aβ resulted in no decrease in the numbers of dendrites or total neurons — two of the noted pathological changes in AD.

Charters that succeed with low - income children go full bore: all - out culture building in the classroom, students and staffers willing to endure longer school hours, bright teachers willing to adapt to precise training regimens and relinquish a fair amount of privacy (giving out their cell - phone number for afterschool homework questions, for example).

Given the number of white blood cells in the urine, urinary culture would be useful but the white blood cells may be inflammatory in nature.

Culturing also allows you to increase the number of cells so that therapy can be more effective.