While CRISPR / Cas9 is a powerful technique for genome manipulation, two significant challenges remain:
obtaining efficient delivery of the Cas9 / sgRNA complex to all cell types, and leaving no additional footprint (i.e., persistent and elevated expression of Cas9 in target cells) that could lead to off - target effects.
Not exact matches
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Lab Assistant — Parker Children's Hospital — March 2007 — May 2012 • Prepared specimens for analysis and distribution following laboratory procedures; performed laboratory functions and worked with quality and regulations indicators •
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