Sentences with phrase «of oligos»

A pair of oligos for each targeting site (Table S1) were annealed and ligated into linearized pX330 vector for generating gRNA - expressing plasmid.
He brought it into Illumina, got it working, licensed it out to the oligo manufacturers, brought the price of oligos down by like 90 % and dramatically increased supply» — which enabled the now — $ 24 billion - in - market cap Illumina to succeed.

Not exact matches

And we can only sell twelve of them a year because there's not enough oligos in the world.
The hope was that antisense oligos for everything from cancer to HIV would roll off the pharmaceutical assembly line — but despite a generation of valiant effort, the successes on this front have been meager.
(FODMAP is an acronym for fermentable oligo - di - monosaccharides and polyols, which is the name of the carbohydrate in question.)
Sometimes lactobacilli need a bit of help getting hold in the intestines, and some practitioners recommend FOS (fructo - oligo - saccharides) to enhance colonization.
After tinkering with several ingredients, they struck gold with a mixture of two chemicals, oligo (e-caprolactone) dimethacrylate and n - butyl acrylate.
A new Oligo - Miocene marsupial lion from Australia and revision of the family Thylacoleonidae.
Levels of APOE protein dropped by about half in mice given the APOE compound as compared with those that received the placebo oligo or saltwater.
Each molecular group includes tumors from all grades and categories of astrocytoma, oligodendrocytoma and oligo - astrocytoma
We put our starting molecule» — a structure called oligo - enediyne, composed of three benzene rings linked by carbon atoms — «on a silver surface, and then induced reactions by heating it.»
I use one of the fancy pipettes to move the oligo into the plasmid tube, and I also add buffer, water, and an enzyme.
If all goes well, the enzyme will cut open the plasmid, removing a piece of its DNA and allowing the oligo to take its place.
After four rounds of selection, the enriched oligo pool was sequenced, and a position weighted matrix was generated for the 12 bp target site and one flanking residue per side (faded).
MyOPGP data has facilitated the development of important genomic platforms, such as single nucleotide polymorphism (SNP), oligo and methylation arrays.
cDNA synthesized by reverse transcription of 1 µg RNA primed with oligo (dT) and random 9 - mer primers was used as the substrate for quantitation of mRNA expression levels by quantitative RT — PCR in the presence of SYBR ® Green (Stratagene, La Jolla, CA).
Specifically, reverse transcription of 5 µg total RNA primed with an oligo (dT) primer bearing a T7 promoter was followed by in vitro transcription, generating multiple antisense copies of each mRNA in the sample (Ambion, Austin, TX).
His clinical research focuses on development of novel diagnostics and therapeutics for oligo - metastatic cancer.
Here we utilized BD ™ AbSeq, a novel protein sequencing approach enabled by oligo - conjugated antibodies, for simultaneous detection of mRNA and protein in single cells.
Reverse transcription was performed on 1 μg of total RNA with an oligo (dT) primer (Roche) and M - MLV RT (Invitrogen).
To further prove the specificity of the binding of STAT6 to the COX - 2 promoter sequence, electrophoretic mobility shift assays (EMSA) were performed utilizing a 30 bp oligo from the COX - 2 promoter sequence encompassing the STAT6 binding site as a probe.
The Glycolytic Versatility of Bacteroides uniformis CECT 7771 and Its Genome Response to Oligo and Polysaccharides
Most likely symptoms of «gluten sensitivity» are actually caused by an herbicide (weed - killer) and a group of carbohydrates known as fermentable oligo - di - monosaccharides and polyols, or FODMAPs for short.
They reiterate that all of the following foods can precipate digestive symptoms in IBS patients: dairy, foods which are high in FODMAPS (fermentable oligo -, di -, monosaccharides, and polyols), high fat and spicy foods, foods with high levels of biogenic amines, i.e. histamine (such as soy), lectins (present in beans), and preservatives, such as benzoic acid and sulfites.
These carbohydrates are part of the FODMAPs (acronym means: Fermentable Oligo -, Di -, and Mono - saccharides And Polyols).
Oligosaccharides [Greek oligo = few; sacchar = sugar] are carbohydrates composed of 3 to 9 monosaccharides.
FODMAPs (Fermentable Oligo - saccharides, Disaccharides, Mono - saccharides and Polyols) are a group of dietary sugars which are poorly absorbed in the small intestine.
Reddy BS, Hamid R, Rao CV Effect of dietary oligo fructose and inulin on colonic pre-neoplastic aberrant crypt foci inhibition.
It is important for you as a consumer to be aware that there are several enzyme / probiotic candida formulations in addition to the many different kinds of pro-biotics that contain «pre» biotics such as fructo - oligo - saccharides (FOS) or inulin fiber.
FODMAP stands for «Fermentable Oligo -, Di -, Mono - saccharides And Polyols,» which is a bunch of fancy talk for a short chain carbohydrate that is poorly absorbed in the small intestine.
(FODMAPs — fermentable oligo -, di - and monosaccharides and polyols — are poorly absorbed carbs found in wheat, dairy, legumes, certain fruits and vegetables, and artificial sweeteners; consult a dietitian if you're thinking of going low FODMAP.)
As public health ministries become accustomed to CombiMatrix's technology, there is a possibility that CBMX could win a large contract anywhere from $ 10m to $ 100m could be awarded to CombiMatrix based on an average cost of the CBMX's «in - house» oligo array automated system, which costs around $ 200 - 300k per unit plus additional fees for customizable chips.
It's not a diet per se but a process whereby you eliminate all FODMAPs (a group of carbohydrates named FODMAPs which stands for Fermentable Oligo - saccharides, Disaccharides, Mono - saccharides and Polyols) and then slowly reintroduce them to see which ones are causing you grief.
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