A pair
of oligos for each targeting site (Table S1) were annealed and ligated into linearized pX330 vector for generating gRNA - expressing plasmid.
He brought it into Illumina, got it working, licensed it out to the oligo manufacturers, brought the price
of oligos down by like 90 % and dramatically increased supply» — which enabled the now — $ 24 billion - in - market cap Illumina to succeed.
Not exact matches
And we can only sell twelve
of them a year because there's not enough
oligos in the world.
The hope was that antisense
oligos for everything from cancer to HIV would roll off the pharmaceutical assembly line — but despite a generation
of valiant effort, the successes on this front have been meager.
(FODMAP is an acronym for fermentable
oligo - di - monosaccharides and polyols, which is the name
of the carbohydrate in question.)
Sometimes lactobacilli need a bit
of help getting hold in the intestines, and some practitioners recommend FOS (fructo -
oligo - saccharides) to enhance colonization.
After tinkering with several ingredients, they struck gold with a mixture
of two chemicals,
oligo (e-caprolactone) dimethacrylate and n - butyl acrylate.
A new
Oligo - Miocene marsupial lion from Australia and revision
of the family Thylacoleonidae.
Levels
of APOE protein dropped by about half in mice given the APOE compound as compared with those that received the placebo
oligo or saltwater.
Each molecular group includes tumors from all grades and categories
of astrocytoma, oligodendrocytoma and
oligo - astrocytoma
We put our starting molecule» — a structure called
oligo - enediyne, composed
of three benzene rings linked by carbon atoms — «on a silver surface, and then induced reactions by heating it.»
I use one
of the fancy pipettes to move the
oligo into the plasmid tube, and I also add buffer, water, and an enzyme.
If all goes well, the enzyme will cut open the plasmid, removing a piece
of its DNA and allowing the
oligo to take its place.
After four rounds
of selection, the enriched
oligo pool was sequenced, and a position weighted matrix was generated for the 12 bp target site and one flanking residue per side (faded).
MyOPGP data has facilitated the development
of important genomic platforms, such as single nucleotide polymorphism (SNP),
oligo and methylation arrays.
cDNA synthesized by reverse transcription
of 1 µg RNA primed with
oligo (dT) and random 9 - mer primers was used as the substrate for quantitation
of mRNA expression levels by quantitative RT — PCR in the presence
of SYBR ® Green (Stratagene, La Jolla, CA).
Specifically, reverse transcription
of 5 µg total RNA primed with an
oligo (dT) primer bearing a T7 promoter was followed by in vitro transcription, generating multiple antisense copies
of each mRNA in the sample (Ambion, Austin, TX).
His clinical research focuses on development
of novel diagnostics and therapeutics for
oligo - metastatic cancer.
Here we utilized BD ™ AbSeq, a novel protein sequencing approach enabled by
oligo - conjugated antibodies, for simultaneous detection
of mRNA and protein in single cells.
Reverse transcription was performed on 1 μg
of total RNA with an
oligo (dT) primer (Roche) and M - MLV RT (Invitrogen).
To further prove the specificity
of the binding
of STAT6 to the COX - 2 promoter sequence, electrophoretic mobility shift assays (EMSA) were performed utilizing a 30 bp
oligo from the COX - 2 promoter sequence encompassing the STAT6 binding site as a probe.
The Glycolytic Versatility
of Bacteroides uniformis CECT 7771 and Its Genome Response to
Oligo and Polysaccharides
Most likely symptoms
of «gluten sensitivity» are actually caused by an herbicide (weed - killer) and a group
of carbohydrates known as fermentable
oligo - di - monosaccharides and polyols, or FODMAPs for short.
They reiterate that all
of the following foods can precipate digestive symptoms in IBS patients: dairy, foods which are high in FODMAPS (fermentable
oligo -, di -, monosaccharides, and polyols), high fat and spicy foods, foods with high levels
of biogenic amines, i.e. histamine (such as soy), lectins (present in beans), and preservatives, such as benzoic acid and sulfites.
These carbohydrates are part
of the FODMAPs (acronym means: Fermentable
Oligo -, Di -, and Mono - saccharides And Polyols).
Oligosaccharides [Greek
oligo = few; sacchar = sugar] are carbohydrates composed
of 3 to 9 monosaccharides.
FODMAPs (Fermentable
Oligo - saccharides, Disaccharides, Mono - saccharides and Polyols) are a group
of dietary sugars which are poorly absorbed in the small intestine.
Reddy BS, Hamid R, Rao CV Effect
of dietary
oligo fructose and inulin on colonic pre-neoplastic aberrant crypt foci inhibition.
It is important for you as a consumer to be aware that there are several enzyme / probiotic candida formulations in addition to the many different kinds
of pro-biotics that contain «pre» biotics such as fructo -
oligo - saccharides (FOS) or inulin fiber.
FODMAP stands for «Fermentable
Oligo -, Di -, Mono - saccharides And Polyols,» which is a bunch
of fancy talk for a short chain carbohydrate that is poorly absorbed in the small intestine.
(FODMAPs — fermentable
oligo -, di - and monosaccharides and polyols — are poorly absorbed carbs found in wheat, dairy, legumes, certain fruits and vegetables, and artificial sweeteners; consult a dietitian if you're thinking
of going low FODMAP.)
As public health ministries become accustomed to CombiMatrix's technology, there is a possibility that CBMX could win a large contract anywhere from $ 10m to $ 100m could be awarded to CombiMatrix based on an average cost
of the CBMX's «in - house»
oligo array automated system, which costs around $ 200 - 300k per unit plus additional fees for customizable chips.
It's not a diet per se but a process whereby you eliminate all FODMAPs (a group
of carbohydrates named FODMAPs which stands for Fermentable
Oligo - saccharides, Disaccharides, Mono - saccharides and Polyols) and then slowly reintroduce them to see which ones are causing you grief.