Performance
of a microfilariae test is recommended at the time the antigen test is performed (6 mo after the adulticide treatment).
Not exact matches
Finally, although Seamus
tested positive for heartworms, because
of his age (Dr. Levy estimates he still young, probably about a year old), it is unlikely that the heartworm
microfilaria (the heartworm larvae spread through the bite
of a mosquito) has had an opportunity to develop into advanced heartworm disease.
That's the beauty
of testing every 4 months... you know it takes at least 5 months for the
microfilaria to develop into the harmful adult heartworms, so if your dog's
test is positive, you can just give him regular heartworm meds for a bit or, if you're holistically minded like me, you can treat him holistically with the help
of your holistic or homeopathic vet.
Many veterinarians prefer to do both
tests as the absence
of microfilariae in the blood does not necessarily mean that there are no adult worms in the heart.
Before giving your dog any kind
of heartworm drugs, you can do DNA
testing to see if there are any
microfilariae in his system.
For example, an infected dog within the household may be the source
of heartworm and therefore should be
tested for heartworm antigen and
microfilariae.
There is generally no need to
test a dog for antigen or
microfilariae prior to ~ 7 mo
of age.
A simple blood
test can be performed by your veterinarian to detect the presence
of immature heartworms (
microfilaria) in your pet.
Rather than visually searching for the
microfilaria, blood screening antigen
tests can detect the presence
of adult female worms even before they've had babies.
Puppies can be infected with
microfilariae and not show signs
of disease even when
tested for many months.
And, finally, in any dog with a very low heartworm burden — say, just a couple
of worms — the
microfilariae may not be detected (this can also cause a false negative on the antigen
test).
However, starting prevention without
testing can be harmful, because once the heartworms reach a certain point in development, the killing off
of the
microfilaria in combination with juvenile or adult heartworm in the system can be damaging to your dog's cardiovascular system.
Heartworm
tests can not show a positive result until the tiny
microfilaria reach 6 - 7 months
of development.
The modified Knott
test and millipore filtration are more sensitive because they concentrate
microfilariae, improving the chance
of diagnosis.
Two negative
tests 7 days apart can provide reasonable assurance
of a lack
of circulating
microfilariae and reduced risk
of transmission.
Testing for the presence
of microfilariae by reviewing blood smears is also used to detect early infection, the period before the adult heartworms has established residence in the arteries or heart and begun reproducing.
Since the primary method
of diagnosis is detection
of these
microfilaria, either visually, or with monoclonal antibody
tests, it is possible to miss a diagnosis.
The preventative won't affect antigen
test results and it can prevent the spread
of heartworm in the shelter and prevent infection in an animal, even if
microfilaria (heartworm larvae) are already present in the bloodstream.
In Germany, canine dirofilariasis has been discovered on routine blood
tests for
microfilaria in 13 %
of dogs with history
of travel to Italy, Portugal, Spain and Corsica and in 10 %
of dogs imported from Italy, Spain and Portugal (Schrey, 1996).
Consequently, the search
of microfilariae by Knott or filtration
tests is still strongly recommended in such places, preferably combined with serological
tests.
In such cases, accurate concentrations
tests, however, can demonstrate circulating
microfilariae in many dogs, avoiding wrong diagnosis and diffusion
of dirofilariasis to newly colonized areas.
A negative concentration
test, does not however, rule out a diagnosis
of dirofilariosis because reports say that 20 to 30 per cent
of canine infections and the majority
of infected cats are negative for
microfilaria (Davoust and Ducos de Lahitte, 1989; Rawlings and McCall, 1982; Rawlings and Calvert, 1995).
1) Dirofilaria immitis
microfilaria (x10, filtration
test) in the blood
of a seronegative symptomatic dog from the non-endemic area
of Fermo (Marche, Italy)
It is generally thought that concentration
tests (Knott, filtration) are the most sensitive, because detection
of microfilaria in the blood is diagnostic for the presence
of adults in the pulmonary arteries (BSAVA, 1998).
As a matter
of fact, none
of seven dogs from a non-endemic area in Central Italy (Fermo, Marche), with D. immitis
microfilariae (fig. 1,2,4) in the blood and patent disease, proved seropositive with two antigen
tests (PetChek and Wittness).
Diagnosis
of canine dirofilariosis in animals which originate from a low - endemic region is difficult for 3 main reasons: 1) possible absence
of circulating
microfilariae (occult disease), 2) high frequency
of a low number
of adults, which in turn produces false negative results, using either ELISA or latex agglutination
test, 3) possible false sero - positive results, due to cross-reaction with adult antigens
of Dirofilaria (Nochtiella) repens (Schrey, 1996), the agent
of subcutaneous dirofilariasis causing chronic pruritic dermatitis in dogs (Tarello, 1999).
The reliability
of heartworm
testing, which depends on the presence
of heartworm antigen or
microfilariae, is affected by the relative lack
of both in cats.
4) Detail
of the caudal end
of a Dirofilaria immitis
microfilaria (x40, filtration
test), from a seronegative and symptomatic dog living in non-endemic area (Fermo, Marche, Italy).
This observation suggests that the search for
microfilariae must not be abandoned, and preferably coupled with serological
tests, which are important tools in enzootic regions, providing semiquantitative data upon the parasitic burden
of infection (Rawlings and Calvert, 1995).
This
tests for the presence
of the offspring (
microfilaria)
of heartworms from a blood sample.
Exclusion
of D. immitis, the causative agent
of heartworm disease, through a negative antibodies
test was an important confirmation that
microfilariae observed were only due to D. repens.
Microscopic examination
of six slides from every Knott concentration
test was carried out and Dirofilaria (Nochtiella) repens
microfilariae were found in the blood
of all cats (Fig 2), their count ranging from 5 to 17 per sample.
An annual blood
test to determine the presence
of microfilariae should be done before beginning the preventive medication.
Clinical re-examination and a Knott
test, carried out 1 month after the completion
of treatment, showed the resolution
of the cutaneous lesions and the absence
of Dirofilaria repens
microfilariae in the blood.
If this
test is negative, the animal still might be infected, because up to 25 %
of dogs with heartworm disease don't have identifiable
microfilariae in their blood.
If the antigen screening
test is positive, another blood
test is available to identify the concentration
of microfilariae in circulation.
A blood sample can be
tested for the presence
of microfilariae.
Most veterinary clinics can run an Antigen
test in the hospital to detect
microfilaria in dogs; however, detection
of heartworm is more difficult in cats and requires an Antibody
test that is usually sent to an outside laboratory.