Sentences with phrase «of adipocytes with»

Based on the gene expression profile, we demonstrated the up - regulation of hormone sensitive lipase and enhancement of the lipolytic activity by the treatment of adipocytes with C3G or Cy.

A total of 633 or 427 genes was up - regulated (> 1.5-fold) by the treatment of adipocytes with C3G or Cy, respectively.

Not exact matches

Differences in release of insulin and other pancreatic and gut hormones have also been observed between breastfed and formula - fed infants, with formula feeding leading to higher plasma levels of insulin which in turn would stimulate fat deposition and early development of adipocytes, the cells that store fat (18).

This process includes the removal of the milk - producing epithelial cells, whereby the alveolar epithelium undergoes apoptosis and is replaced with adipocytes.

After 2 years as a resident at the University of Chicago Hospitals, Sargis went to work with Matthew Brady, an endocrinologist in the University of Chicago's Pritzker School of Medicine, studying the development of adipocytes, the cells that store fat.

The scientists started with metabolically relevant cells — hepatocytes and adipocytes — representing major functions of the liver and adipose tissues, and thus important aspects of the body's energy processing and storing system.

When macrophages are stimulated with an endotoxin, they produce a factor or factors, termed cachectin, that inhibits the activity of fat - producing (lipogenic) enzymes in cultured adipocytes.

The research team from the Department of Biochemistry and Molecular Biology headed by Professor Susanne Mandrup are publishing a paper entitled «Browning of human adipocytes requires KLF11 and reprogramming of PPAR super-enhancers» in the January 1 edition of the scientific journal Genes & Development that describes their results from working with «brite» fat cells.

It is considered that methods to convert white adipose tissues * 7 into the ones containing beige adipocytes with increased expression of UCP - 1 would lead to prevention and treatment of obesity.

«The idea is that when fat cells (adipocytes) interact with environmental agents — in this case, bacterial toxins — they then trigger a chronic inflammatory process,» says Patrick Schlievert, Ph.D., UI professor and head of microbiology and co-senior author of a new study published in the journal PLOS ONE.

The expression rates of three macrophage - specific genes (Emr1, Cd68, and Csf1) that correlated with body mass in our microarray studies, an adipocyte - specific gene (Acrp30), and proinflammatory genes (Tnfa, Nos2, Il6) were determined by quantitative RT - PCR.

The adipocyte - enriched population (gray bars) expressed small amounts of the macrophage markers (a), consistent with residual macrophage contamination seen by immunofluorescent staining of live cells (b).

These data demonstrate that variations in continuous quantitative traits such as body mass, adipocyte size, and BMI are correlated with quantitative variations in the expression of genes.

Immunohistochemical analysis of perigonadal, perirenal, mesenteric, and subcutaneous adipose tissue revealed that the percentage of cells expressing the macrophage marker F4 / 80 (F4 / 80 +) was significantly and positively correlated with both adipocyte size and body mass.

In his study, Wang found that pups born to mother mice fed a triple dose of Vitamin A were born with more fat - burning brown fat cells, and had more small blood vessels in their adipose tissues, providing precursor cells for energy - burning beige adipocytes

(3,18; cf. 19,20) Even in visceral fat, it has recently emerged that the obesity - driven rise in inflammation and insulin resistance is associated with an abnormal accumulation of senescent cells, albeit senescent endothelial cells rather than adipocytes.

In individuals with T2D, this function is frequently perturbed by an impaired response of the adipocytes to insulin resulting in elevated lipid levels in circulation and storage in alternative tissues such as liver, muscle, and pancreas (3).

Adipogenesis Dye Allows for Visual Confirmation of Adipocyte Differentiation 3T3 - L1 cells were treated with and without induction media.

They were crowded with mitochondria that were much smaller than those of brown adipocytes.

Overgrown tissue was composed of normal - sized, UCP1 - negative unilocular adipocytes, with mitochondrial network fragmentation, disorganised cristae, and increased autophagosomes.

The variants associated with both outcomes implicate new pathways as well as targets of existing drugs, including icosapent ethyl and adipocyte fatty - acid - binding protein.

These findings suggest that specific MFN2 mutations cause tissue - selective mitochondrial dysfunction with increased adipocyte proliferation and survival, confirm a novel form of excess adiposity with paradoxical suppression of leptin expression, and suggest potential targeted therapies.

Conclusions: Our findings demonstrate that disruption of Bscl2 specifically in developing adipocytes is sufficient to cause the early - onset generalised lipodystrophy observed in patients with mutations in BSCL2.

Conversely, when spexin levels are regulated, gut motility improves along with food intake, energy metabolism, and regulation of fat storage, particularly long chain fatty acid uptake in fat cells.6 Walewski, J. L., et al. «Spexin is a novel human peptide that reduces adipocyte uptake of long chain fatty acids and causes weight loss in rodents... continue

Walewski, J. L., et al. «Spexin is a novel human peptide that reduces adipocyte uptake of long chain fatty acids and causes weight loss in rodents with diet ‐ induced obesity.»

Twelve - hour exposure of 3T3 - L1 adipocytes to H (2) O (2) or TNF - alpha resulted in the increase of c - Jun NH (2)- terminal kinase (JNK) activation and insulin receptor substrate 1 (IRS1) serine 307 phosphorylation, concomitantly with the decrease in insulin - stimulated IRS1 tyrosine phosphorylation and cellular glucose uptake.

Treated adipocytes with anthocyanins enhanced adipocytokine (adiponectin and leptin) secretion and up - regulated the adipocyte specific gene expression without activation of PPARgamma in isolated rat adipocytes.

METHODS: We treated 3T3 - L1 adipocytes with 2.5 mmol / l R (+) alpha - lipoic acid for 2 to 60 min, followed by assays of: 2 - deoxyglucose uptake; glucose transporter 1 and 4 (GLUT1 and GLUT4) subcellular localization; tyrosine phosphorylation of the insulin receptor or of the insulin receptor substrate - 1 in cell lysates; association of phosphatidylinositol 3 - kinase activity with immunoprecipitates of proteins containing phosphotyrosine or of insulin receptor substrate - 1 using a in vitro kinase assay; association of the p85 subunit of phosphatidylinositol 3 - kinase with phosphotyrosine proteins or with insulin receptor substrate - 1; and in vitro activity of immunoprecipitated Akt1.

Feeding the HF diet markedly induced hypertrophy of the adipocytes in the epididymal white adipose tissue compared with the control group.

The mice without an adipocyte clock and shifted feeding rhythms developed obesity, as well as changes in the relative amounts of various TGs stored in adipocytes, circulating in the plasma, and present in hypothalamic neurons associated with energy balance (31).