Sentences with phrase «of agarose»

Using acrylamide instead of agarose gives much sharper bands.

If you are analyzing short DNA fragments, run PAGE instead of agarose to get sharper bands.

Using chondroitin sulfate to improve the viability and biosynthesis of chondrocytes encapsulated in interpenetrating network (IPN) hydrogels of agarose and poly (ethylene glycol) diacrylate.

The bioactivity of agarose — PEGDA interpenetrating network hydrogels with covalently immobilized RGD peptides and physically entrapped aggrecan.

He and his colleagues wanted to avoid the classic light - sheet arrangement in which the sample is embedded in a tube of agarose and surrounded by lasers and cameras.

Not exact matches

The design differs from that of a conventional commercial microscope: Keller placed the laser and objective lens at right angles to each other atop a table; then he embedded zebrafish embryos in agarose in a sample tube between the two.

To imitate the cactus root and its outer covering, they made a material composed of cellulose fibers, agarose cyrogel and microparticles.

First, they 3 - D - printed wormlike strands of a gel called agarose, each serving as a cast of a tiny blood vessel.

Conditions for the PCR reaction were 40 cycles of 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s. Amplicons were purified on a 2 % agarose gel, cloned into plasmid vectors using TOPO TA (Invitrogen, Carlsbad, CA), and sent to an outside company (Elim Biopharmaceuticals, Hayward, CA) for Sanger sequencing in both directions using vector primers M13F and M13R.

When an electric current is applied along the length of the gel, the DNA starts to migrate through the agarose thicket.

A 3 - ml overlay consisting of EMEM with 0.4 % agarose was added, and the cells were incubated at 37 °C for 72 hours.

Preparation and characterization of superporous agarose — reticulated vitreous carbon electrodes as platforms for electrochemical bioassays.

(This is an updated version of the current DNA Fingerprinting field trip, replacing agarose gels with the DNA chip.)

The improved size resolution of the DNA chip allows students to identify their genotype, something impossible with agarose gel electrophoresis.

Cell extracts (1.5 mg) were incubated with 15 μL of anti-FLAG M2 - agarose affinity gel (Sigma - Aldrich, St. Louis, MO).

Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].

The amplified DNA products were analyzed on a 1.0 % agarose gel, and pieces of gel containing the amplified DNA bands were excised and purified using a gel extraction kit (Takara Bio).

Genomic DNA of clones B31 - A3, ospC7, and ospC7 / ospC +4 was separated through agarose gels, transferred to a membrane, and hybridized with a 32P - labeled probe specific for ospC.

c, Southern blot (left) membrane hybridization of 10 µg of BamHI - digested genomic DNA (see corresponding agarose gel on right) using a DNA probe from the pCEP backbone.

To generate a full - length unc - 9:: gfp construct, the 14.7 - kb UNC - 9A + B PCR product (representing the 5» end of unc - 9) and the unc - 9:: gfp plasmid were digested separately with Kpn I, digests electrophoresed through low - melt agarose (SeaPlaque GTG agarose, FMC, Rockland, ME, USA), and appropriate fragments purified by phenol extraction and ethanol precipitation.

This product was similarly purified in a low - melt agarose gel and used at 1 ng / μl along with 50 ng / μl of the unc - 36 (+) cosmid derivative RIp16 (gift of L Lobel) for microinjection into unc - 36 -LRB--) animals.

The supernatant was mixed with 300 µl of Ni - NTA agarose (Qiagen), and the proteins were allowed to bind for 3 hours, followed by washing with 20 ml of resuspension buffer, and eluted with 500 µl of elution buffer (20 mM NaHPO4, 300 mM NaCl, 10 % glycerol, 150 mM Imidazole, pH 7.8).