Sentences with phrase «of agarose gel»
Not exact matches
First, they 3 - D - printed wormlike strands of a gel called agarose, each serving as a cast of a tiny blood vessel.
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Conditions for the PCR reaction were 40 cycles of 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s. Amplicons were purified on a 2 % agarose gel, cloned into plasmid vectors using TOPO TA (Invitrogen, Carlsbad, CA), and sent to an outside company (Elim Biopharmaceuticals, Hayward, CA) for Sanger sequencing in both directions using vector primers M13F and M13R.
When an electric current is applied along the length of the gel, the DNA starts to migrate through the agarose thicket.
(This is an updated version of the current DNA Fingerprinting field trip, replacing agarose gels with the DNA chip.)
The improved size resolution of the DNA chip allows students to identify their genotype, something impossible with agarose gel electrophoresis.
Cell extracts (1.5 mg) were incubated with 15 μL of anti-FLAG M2 - agarose affinity gel (Sigma - Aldrich, St. Louis, MO).
Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].
The amplified DNA products were analyzed on a 1.0 % agarose gel, and pieces of gel containing the amplified DNA bands were excised and purified using a gel extraction kit (Takara Bio).
Genomic DNA of clones B31 - A3, ospC7, and ospC7 / ospC +4 was separated through agarose gels, transferred to a membrane, and hybridized with a 32P - labeled probe specific for ospC.
c, Southern blot (left) membrane hybridization of 10 µg of BamHI - digested genomic DNA (see corresponding agarose gel on right) using a DNA probe from the pCEP backbone.
This product was similarly purified in a low - melt agarose gel and used at 1 ng / μl along with 50 ng / μl of the unc - 36 (+) cosmid derivative RIp16 (gift of L Lobel) for microinjection into unc - 36 -LRB--) animals.