Sentences with phrase «of all cell lines used»

I and a colleague have attempted to use this on two different types of cell lines using Flow cytometry and fluorescence microscopy all of which proved unsuccessful.

As names are not even used consistently in publications and deviations from the original name are not rare, the situation increasingly causes confusion about the identity of cell lines used in specific studies.

Approximately 20 - 30 % of all cell lines used in biomedical research are contaminated.

Not exact matches

The majority of study participants reported that it's all good, fine and kosher with them (well, technically «generally OK») to use a cell phone when walking down the street, riding public transit and waiting in line.

Phone surveys used to be the standard in the industry back when all customers had land lines, but in the era of the cell phone and do - not - call list, it's less reliable.

Memphis Meats acknowledges it used FBS to start its cell lines but says, «We have validated a production method that does not require the use of any serum, and we are developing additional methods as we speak.»

In August of last year, President Bush approved the use of federal funds to support research on a limited number of existing human embryonic stem cell lines.

Where is the clear line in a progression from (1) using animal insulin to treat diabetes, to (2) using gene remodeling techniques to grow insulin in a host bacterium that will reproduce rapidly and from which a plentiful supply of insulin can be harvested, to (3) genetic surgery to replace the defective gene in a person diagnosed as diabetic, to (4) genetic surgery immediately after fertilization in order to replace the defective gene and alter the germ cells which would otherwise have transmitted the disease to one's offspring?

The second part of the automation job was the complete process data acquisition for the filling process cells that cover the data processing for seven filling lines, with approximately 150 associated controllers used to cyclically read 21,000 digital and 2,000 analogue data points.

Our bodies then use these essential nutrients to rebuild cells and particularly the lining of our intestine — which prevents those food sensitivities that seem to be on the rise these days.

Before we established in - house human taste cell (HTC) technologies, it was not possible to use human tongue - derived cells as a model, due to the lack of homogenous, proliferating cell lines with defined properties, which is a prerequisite to establish comprehensive research and screening programs.

As well as allowing the use of stem cells grown from established cell lines, the technology could enable the creation of improved human tissue models for drug testing and potentially even purpose - built replacement organs.

Using an experimental FA cell line, they also found that cells without frataxin produced lower levels of ApoA - I compared to controls.

To solve this, West proposed «therapeutic cloning» — taking the nucleus out of a patient's cell, transferring it into an egg cell to create a cloned embryo, then using that embryo to derive patient - matched stem - cell lines.

One perplexing problem, however, is that three out of 13 of the iPS cell lines created by Hochedlinger's team had a complete extra set of chromosomes, which has not been seen with iPS cells made using retroviruses.

Each of the four compounds was then tested in the lab to see how it would affect a line of prostate cancer cells relatively sensitive to the chemotherapeutic Paclitaxel, commonly used to treat prostate cancer patients.

In these instances, the committee says, the use of existing embryonic cell lines derived from embryos fewer than 14 days old should be considered before resorting to the use of cells taken from surplus in vitro fertilization embryos fewer than 14 days old.

In addition, where cells derived from embryonic stem cells are great at proliferating — a potentially critical feature if one wants to grow sufficient numbers of cells for clinical use — ones from the iPS lines were much feebler.

Figure on the left: Analyzing the relative sensitivity of hundreds of cancer cell lines to commonly used anti-cancer drugs (each dot) reveals that cells with suppressed expression of one proteasome cap subunit are specifically resistant to treatments with the proteasome inhibitors MG132 and bortezomib.

Doing this in bacteria or cell lines used for growing chemicals would be of huge importance to industry, where viral infections can shut down entire production lines.

And early stage startup Neochromosome, which includes Boeke, intends to raise money to design synthetic chromosomes for medicine that could be used in an off - the - shelf universal cell line in cell therapies and transplants with minimal risk of rejection from the immune system.

The enzyme was tested in three different cell lineages: leukemia cells incapable of producing asparagine at normal levels (MOLT4); another line of leukemia cells, in this case capable of producing asparagine at normal levels (REH); and non-malignant cells, used as a control (HUVECs).

Hamilton announced the newest organ - on - chip innovation, which recreates an intestinal lining using patient - derived stem cells, created through a partnership between Emulate and Cedars - Sinai Board of Governors Regenerative Medicine Institute.

The team also analyzed the effects of IL - 10 on epithelial wound closure in vitro using an intestinal epithelial cell line.

A stem cell core facility at CHOP, directed by study co-author Deborah French under the auspices of the hospital's Center for Cellular and Molecular Therapeutics, generated the iPSCs lines used in these studies.

Using both fruit fly and human lung cancer cell lines, researchers targeted two of the most common genetic mutations associated with NSCLC — Ras and PTEN (P13K).

His team plans to introduce a healthy copy of the CF gene into cells lining the nose and lungs, using a lipid suspension that fuses with the cell membrane and allows the gene to enter the cell.

To see whether cancer stem cell renewal involves a chain of events similar to that used by embryonic stem cells, and whether the process was affected by oxygen levels, Semenza and graduate student Chuanzhao Zhang focused their studies on two human breast cancer cell lines that responded to low oxygen by ramping up production of the protein ALKBH5, which removes methyl groups from mRNAs.

The laboratory process, described in the journal Scientific Reports, entails genetically modifying a line of human embryonic stem cells to become fluorescent upon their differentiation to retinal ganglion cells, and then using that cell line for development of new differentiation methods and characterization of the resulting cells.

While American stem cell researchers were celebrating the final NIH stem cell guidelines, questions remain, particularly whether the National Institutes of Health will approve existing lines in time for use with stimulus funds.

The team used five types of antibiotics — including one used to treat acne (doxycycline)-- on cell lines of eight different types of tumour and found that four of them eradicated the cancer stem cells in every test.

Stem cell advocates have been expressing serious worry that ethical requirements spelled out in the draft guidelines — in particular, informed consent procedures for embryo donors — will rule out the use of many existing human embryonic stem cell lines, including the 21 lines approved under the Bush Administration.

The final guidelines on research with human embryonic stem cells issued on Monday by the National Institutes of Health set out criteria for determining which ES cell lines can be used in federally funded experiments and give NIH discretion to approve old lines that don't meet stringent modern ethical requirements.

Last January, the House of Representatives voted, 253 to 174, to pass a bill, H.R. 3, that would allow researchers to use leftover embryos from in vitro fertilization (IVF) clinics to create new lines of embryonic stem cells, and in April, the Senate passed its version of the bill.

Using nanoparticles designed and screened for endothelial delivery of short strands of RNA called siRNA, the researchers were able to target RNAi to endothelial cells, which form the linings of most organs.

They have since learned that as many as 40 of those lines may never be fully developed; some may even have been contaminated by mouse cells used to sustain them in the lab.

Mindful of public sensitivities, Daley opted to pursue experiments using what he considers the least controversial human materials to create new nonpresidential stem cell lines — poor quality embryos and oocytes that, in his words, «otherwise would have been disposed of as medical waste.»

Using a powerful imaging technique that allowed the scientists to track the presence and movement of parasites in living tissues, the researchers found that Toxoplasma infects the brain's endothelial cells, which line blood vessels, reproduces inside of them, and then moves on to invade the central nervous system.

A team of researchers in Germany and Denmark led by Steven Johnsen, Professor at the University Medical Center Göttingen, Germany, used human prostate cancer cell lines and depleted them of the DNA - binding protein CHD1.

Cheng, an assistant professor of medicine in hematology / oncology at Feinberg, provided the cell lines and NanoFlare targets the researchers used to model blood samples taken from breast cancer patients.

One of the most popular models is the immunodeficient nonobese diabetic severe combined immunodeficiency (NOD scid) gamma (NSG) transgenic mouse line, which can be endowed with a humanized immune system using CD34 + hematopoietic stem cells.

The scientists used the new indexing method on several human cell lines and from a mouse brain to reveal the methylome of 3,282 single cells.

Henrietta Lacks famously (and unwittingly) established the HeLa cell line, one of the most widely used lines in cancer research.

Researchers have already developed dozens of patient - specific cell lines using this new method.

They used the gene editing technology CRISPR to engineer a series of human embryonic stem cell lines, which were identical apart from the number of DNA repeats that occurred at the ends of their HTT genes.

To address these questions, Dang, Altman, and Hsieh collaborated with Penn colleague, Aalim Weljie, PhD, research assistant professor of Pharmacology, using an osteosarcoma cancer cell line to study the interaction of MYC and metabolism in cancer cells.

The team used light and electron microscopy to show that lymphatic endothelial cells, which line the interior surface of the lymph node capsule, contain a number of channels that penetrate the whole thickness of the cells.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gcell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gCell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

Earlier approaches to creating this growth environment, pioneered by study co-author Jason Spence, Associate Professor of Internal Medicine at the University of Michigan, had used a natural matrix derived from a tumor cell line.

A new technique of direct binding of radioactive DNA was used to screen a complementary DNA expression library from the BJAB cell line in lambda gt11 phage to derive molecular cDNA clones representing a putative B lymphocyte - specific octamer binding protein.