Sentences with phrase «of allografts»

To study infiltration of allografts, frozen sections were stained with peroxidase - conjugated antibodies against either CD4 or CD8 as recommended by the manufacturer (BD Biosciences, San Diego, CA).

To determine the histology of the allografts, we sacrificed our transplanted animals on either day 40 or day 100 post-transplantation.

Reconstitution with syngeneic plus allogeneic or xenogeneic bone marrow leads to specific acceptance of allografts or xenografts.

Allospecific CTL and DTH responses have been associated with the immune rejection of various categories of allografts.

This study was also of only one type of allograft (Tibialis Posterior).

Overall, MSCs can exert protective effects in ischemic reperfusion injuries through anti-inflammatory and paracrine factors and this likely plays an important part in MSC enhancement of allograft survival.

APOL1 genetic testing can help you make informed decisions about how to manage future risks of allograft failure in your renal transplant patients.

To determine a possible impact of the allograft on mixed chimerism, we measured the levels of bone marrow mixed chimerism 40 days post cardiac graft transplantation.

This preparation provides access to the patient's own stem cells, which may undergo osteogenic differentiation in response to delivery of the allograft BMDMSC.

Not exact matches

A study in 2011 looking at more than 645 ACL surgeries found that in patients between the ages of 10 ««19, allograft tissue had a four times higher failure rate than using your own tissue.

This may entail making small holes in the bone to allow new cartilage to grow (microfracture), taking cartilage from another part of the athlete's knee and transplanting it into the defect (osteochondral autograft transfer), taking cartilage cells from the knee and then having them grown in a lab for later re-implantation (autologous chondrocyte implantation), or taking cartilage from a person who has passed away and placing it in the defect (osteochondral allograft transfer).

Correlation of leukocyte groups with skin and renal allograft survival indicates that ranks of histocompatibility based upon current genetic concepts of the HL - A system may provide an approach to the selection of optimally compatible subjects for clinical organ transplantation.

The study, «Optimizing Femoral - head Osteochondral Allograft Transplantation in a Preclinical Model,» recently was published in the Journal of Orthopaedic Translation.

The rate of early allograft dysfunction — a serious and potentially deadly complication of transplantation — occurred in only 10 % of machine - stored liver recipients, versus 30 % of those allocated ice - stored ones.

Using an approach developed at Maisonneuve - Rosemont, consisting of an autograft to reduce tumour mass followed by a family allograft three to four months later to clean the bone marrow of myeloma cells with immune cells from a family donor (immunotherapy), the study resulted in a total cure rate of 41 %, a record level using this strategy.

Moreover, patients in complete remission six months after the allograft had a relapse - free survival rate of 60 %.

Overall, the autograft strategy followed by allograft resulted in relapse - free survival rates of 20 - 25 % in the long term.

Such allografts have already been performed: a portion of respective tissue is being recovered from a dead donor and placed under the mucosa of the recipient's stomach.

«Long - term prevention of organ rejection: Biologists use immunoproteasome inhibition to prevent chronic antibody - mediated allograft rejection.»

Several mTOR inhibitors have already been approved for therapeutic use, in particular in the treatment of cancer and allograft rejection.

In the present study we sought to determine the role and mechanisms of CD4 - independent rejection of corneal allografts.

However, closer scrutiny of these studies raises questions about the role of CD4 + T cells as the sole mediators of corneal graft rejection, as corneal allografts undergo immune rejection in 33 % of the mice and 64 % of the rats treated with anti-CD4 monoclonal antibody (12, 13) and in 45 % of the CD4 KO mice (14).

BALB / c corneal allografts were transplanted 24 hr later and the fate of the corneal allografts determined.

Interestingly, recipients of CD8 + T lymphocytes failed to mount DTH responses to BALB / c alloantigens (Figure 4), even though similar panels of mice rejected 95 % of their BALB / c corneal allografts (Figure 2B).

Immunohistochemical staining of rejected human corneal allografts has revealed the presence of apoptosis in corneal stromal cells (5).

The capacity of CD8 − T cells from CD4 KO donors to mediate corneal allograft rejection is puzzling and on the surface, counterintuitive, since these cells are presumably double negative (DN) T cells.

Either of these effector mechanisms might contribute to corneal allograft rejection.

An adoptive transfer experiment was performed to confirm that the high incidence of corneal allograft rejection was immune - mediated.

By contrast, 71 % (5/7) of the beige nude mice that received spleen cells from CD4 KO mice rejected their BALB / c corneal allografts.

Removing one immune effector element only reveals the presence of an ancillary pathway for allograft rejection.

The present demonstration of T cell - mediated apoptosis of allogeneic corneal cells from CD4 KO mice is consistent with previous findings, which noted the presence of apoptotic keratocytes and corneal endothelial cells in rejected corneal allografts in humans and rats respectively (5, 32).

BALB / c corneal allografts underwent rejection in 46 % (6/13; MST = 46 days) of the recipients of CD8 − T lymphocytes and in 95 % (19/20; MST = 15 days) of the recipients of CD8 + T lymphocytes (Figure 2B).

The results of a typical experiment are shown in Figure 3 and demonstrate that even though spleen cells from CD4 KO mice could adoptively transfer corneal allograft rejection, they did not display conventional CTL activity against either BALB / c corneal epithelial or endothelial cells.

T lymphocytes are required for the rejection of corneal allografts, as athymic, T cell - deficient nude mice do not reject corneal allografts (3).

The role of DN T cells in corneal allograft rejection was confirmed in two separate in vitro assays in which CD8 − cells were isolated from CD4 KO donors that had rejected corneal allografts and were found to induce apoptosis of donor - specific corneal cells.

The combination of anti-NKG2D and CTLA - 4 Ig therapy prolongs islet allograft survival in a murine model.

Indeed, our results indicate that adoptive transfer of CD8 + T cells from CD4 KO mice that had rejected corneal allografts resulted in the rejection of 95 % of the corneal allografts transplanted to athymic recipients.

The capacity of CD8 + T cells to mediate corneal allograft rejection could have been due to the CTL population that might have been present in the CD8 + T cell suspensions used in the adoptive transfer inocula.

Histopathological examination of rejected corneal allografts in CD4 KO mice revealed a mixed inflammatory infiltrate containing large numbers of neutrophils and mononuclear cells, which was indistinguishable from the infiltrate seen in rejected corneal allografts in wild - type mice (data not shown).

Several studies suggest that a significant number of corneal allografts undergo rejection in the absence of CD4 + T cells.

CD8 + and CD8 − T cells were collected from either C57BL / 6 mice or adoptive cell transfer recipients one week after the rejection of BALB / c corneal allografts.

Current projects are investigating: 1) the expression of inflammatory genes during ischemia and reperfusion of kidneys during urology transplantation and in mouse models; 2) the expression of inflammatory genes and proteins in urine as markers indicating the presence of rejection in renal allografts; and 3) the role of adhesion molecules and chemokines in directing leukocyte infiltration into organ allografts.

The results indicated that 54 % (38/70) of the BALB / c corneal allografts underwent rejection in the CD4 KO hosts, while 100 % of the grafts were rejected in wild - type C57BL / 6 mice (Figure 1).

By contrast, adoptive transfer of CD8 − spleens cells from similar donors resulted in the rejection of corneal allografts in almost half of the hosts.

However, some studies have demonstrated a role for CD8 + T cell - mediated rejection of skin and cardiac allografts (16, 17).

Additional adoptive transfer experiments were performed to ascertain the role of CD8 + and CD8 − T lymphocytes in CD4 - independent rejection of corneal allografts.

None of the ten C57BL / 6 beige nude mice rejected BALB / c corneal allografts (Figure 2A).

Insufficient or excess complement activation has been shown to be important in a wide range of disease states, such as multiple sclerosis, myasthenia gravis, systemic lupus erythematosus, rheumatoid arthritis, and age - related macular degeneration, as well as in allograft rejection.

This study examined the role of CD4 + T cell - independent mechanisms of corneal allograft rejection.

Development of DTH responses to donor alloantigens has been correlated with corneal allograft rejection.