Sentences with phrase «of cell culture conditions»

«In the future, this will move us towards a way to understand how to generate a cardiac muscle or other specific cell type without having to try an unwieldy number of cell culture conditions.»

Not exact matches

Under experimental conditions, the researchers then demonstrated that elevated levels of elastase dramatically reduce the survival and operation of muscle stem cells in culture.

Despite a lack of rigorous clinical trials to test safety or efficacy, a ban of the treatment by health authorities, and a lack of peer review by the scientific community, the privately funded Stamina Foundation has been using cultured mesenchymal stem cells that have been exposed to putative conditions that favour neuronal differentiation to treat different diseases in severely or terminally ill patients.

Human chondrosarcoma HCS - 2 / 8 cells, which are a type of benign bone cancer cells, can be used to investigate how optimization of culture conditions could improve the synthesis of cartilage - specific molecules.»

«We also think this method could be applied towards the development of culture conditions during differentiation of human stem cells.

Studies of IBD are typically performed using cell culture experiments or animal models, which don't mimic the precise conditions that occur in the gut of human patients.

«The culture conditions needed to grow various kinds of stem cells can be very tricky,» down to the source of the water, says neural stem cell biologist David Anderson of the California Institute of Technology in Pasadena.

The scientists then tried the proline / polyproline combination in a test system of adherent cell cultures that were subjected to freeze - thaw conditions.

«We isolated kidney progenitor cells from mice and searched for the most favorable culture conditions,» said Assistant Professor Shunsuke Tanigawa of Kumamoto University.

They then combined these in a dish with testicular cells from newborn mice in various culture conditions — a process that took hundreds of trials, says author Xiao - Yang Zhao, a stem cell biologist now at Southern Medical University in Guangzhou, China.

Haruko Obokata of the RIKEN Center for Developmental Biology (CDB) in Kobe, Japan, and colleagues at other Japanese institutions and at Harvard Medical School in Boston reported that simply subjecting blood cells from newborn mice to a moderately acidic environment for 25 minutes and then tweaking culture conditions could generate pluripotent stem cells capable of developing into nearly all of a body's cell types.

They then placed the cells into culture conditions containing certain cytokines, and instead of differentiating into B - cells, the cells continued to divide as stem cells.

«Indeed, this model of cell culture may reproduce what happens under physiological conditions.»

Also, in both cell culture and a mouse model of the condition, blocking the excessive splintering reduced production of reactive oxygen species, a potentially harmful byproduct of metabolism, as well as cell death.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

Phase - contrast images of organ - derived endothelial cells (37 °C culture conditions) that had undergone two successive selection processes by flow cytometry.

The first step of our program is to apply the previously established lab - culture conditions that first commit human ES (hES) and iPS cells into retinal precursors.

The second step will be to optimize culture conditions for the commitment of the iPS / hES cell - derived retinal precursors towards the photoreceptor lineage.

Most of these were invalidated when challenged with experimental data generated in Smith's laboratory, which measured gene expression of ESCs across 23 different cell culture conditions, all of which maintained pluripotency.

Difficult - To - Express Proteins: Resolving Bioprocessing Challenges with a Scalable Perfusion Bioreactor Scott Waginer, Emily Wozniak, and Kyle Biesecker (The Cell Culture Company) Among some other recombinant glycoproteins, bi - and tri-specific antibodies are labeled as «difficult - to - express» proteins because of low titers and sensitivity to environmental conditions.

Bartosz Balana (Ravens, TUD)-- «Influence of culture conditions on differentiation and bioelectrical properties of human mesenchymal stem cells» (2005)

Low - serum conditions for expansion culture of multiple cell types.

Scientists developing new cell therapy methods for the treatment of disorders such as sickle cell disease or leukemia need to consider how their culture conditions may affect the success of their translational research.

Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.

The most well - known of these are so - called «senescent» cells, originally characterized by Leonard Hayflick as mitotic cells that reached growth arrest after a limited replicative lifespan (later associated with telomere attrition) under unphysiological conditions in culture.

Previous studies of cell cultures from diabetic subjects cultured under basal physiological conditions showed that the basal as well as the insulin - stimulated FV0.1 values are reduced (4,5).

Consistent with their previous studies in cell culture, mice exposed to paraquat under realistic conditions developed an increased burden of senescent cells in their brains (Figure 1, (a-c)-RRB--- and this plague was almost entirely restricted to astrocytes (Figure 1 (d)-RRB-.

Previous studies of cell cultures established from diabetic subjects and cultured under basal physiological conditions showed that the basal as well as the insulin - stimulated FV values are reduced (4,5).

To highlight the potentially important role of 3D culture conditions, the authors compared 2D monolayers of hepatocyte progenitors, fetal liver stromal cells, or adult bone marrow - derived stromal progenitor cells to their 3D counterparts cultured on liver extracellular matrix (3D - ECM) constructs.

This ensures that sister cultures from the same subjects are similar in cell composition and differentiation state, and it avoids the influence of experimental conditions on the differentiation process (1).

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

Bone marrow cells cultured with conditioned medium derived from ABL1 / ABL2 knockdown 1833 and SKBR3 breast cancer cells had decreased numbers of TRAP + cells compared to the control groups (Fig. 5, B and C, and fig.

In light of the observation that iPS cell derivation takes place under the same culture conditions used for ES cells [20], we hypothesized that these human feeder cells could offer a stable tool for defining molecular hallmarks during conversion of differentiated somatic cells to the pluripotent state.

(A) Purified CD4 + naive T cells were cultured with different concentrations of VPA under Th1 - polarizing conditions.

Our improved IVM culture conditions may be used for obtaining mature oocytes for clinical purposes and / or for derivation of embryonic stem cells following parthenogenesis or nuclear transfer.

(A, B) Culturing wild - type brains with conditioned media from GBP1 and GBP2 expressing cells induces low level of ILP2 (A) and ILP5 (B) accumulation in the insulin - producing cells.

(C, D) Culturing wild - type brains with conditioned media from GBP1 and GBP2 expressing cells decreases the densities of ILP2 (C) and ILP5 (D) signals in the insulin - producing cells.

No expression of SCP3 was detected in the EBs examined, whereas there was an increase of SCP1 expression in the day 28 EBs cultured in ovarian cell - conditioned medium.

The low levels of Hex transcript observed in undifferentiated ES cells (Figure S2C) were sufficient to generate a significant Venus positive (V +) sub-population in undifferentiated ES cell cultures grown under standard feeder free conditions.

Inverted microscopes represent the perfect solution for cell culture in which a variety of cells are grown under controlled conditions.

HV cells cultured under self - renewing conditions were subjected to flow cytometry to separate Venus positive and negative ES cell subpopulations and injected into Rosa26 LacZ expressing blastocysts within 1 h of purification.

(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.

The current findings could provide important clues to determine the culture conditions for promoting the differentiation of primate ES cells into mature gametes, and to understand molecular mechanisms of primate gametogenesis including the timing of germ cell induction, the regulation of germ cell gene expression, and the response to growth factors for germ cell differentiation.

Our results demonstrated that our 3D cell culture device enables a real - time imaging of giant vacuole formation and tracers crossing the cultured endothelial cell monolayer in a controlled experimental condition.

Culture media for tumor cells were supplemented with 10 % FBS, whereas culture medium for HUVEC was supplemented with 20 % FBS, with the exception of the experiment under low proliferation rate conditions (2 Culture media for tumor cells were supplemented with 10 % FBS, whereas culture medium for HUVEC was supplemented with 20 % FBS, with the exception of the experiment under low proliferation rate conditions (2 culture medium for HUVEC was supplemented with 20 % FBS, with the exception of the experiment under low proliferation rate conditions (2 % FBS).

In the case of EB formation using mouse testicular or ovarian cell - conditioned medium (see below), the EBs were cultured in ES medium for 24 hr, and then in a conditioned medium.

Increased permissivity of monkey cells to human adenovirus multiplication is affected by culturing conditions and correlates with both synthesis of virion fiber protein and altered splicing of its mRNA

The existence of a paracrine inter-dependent equilibrium would suggest that the culture conditions have selected for the stable coexistence of mutually dependent and metastable cell types that only transiently exist in vivo.

VASA was detected earlier in cells cultured in testicular cell - conditioned medium than in those cultured in non-conditioned medium or in ovarian cell - conditioned medium, suggesting that the initiation of germ cell differentiation in testicular cell - conditioned medium was faster than that in ovarian cell - conditioned medium.

As expected the culture of HV cells under these conditions resulted in a significant reduction in the V+S + population (Figure 6B).