Not exact matches
He has more than fifteen years» experience in the life science industry, including the product development, market development and commercialization
of cells, scaffolds,
cell reprogramming tools and
cell culture media for regenerative medicine and bioprocessing applications.
Setton's lab exposed the hiPSCs to a variety
of different growth factors and
culture media to coax them into first developing markers for, and then fully forming into, notochord
cells.
One
of the 12 audience members was Ben Chao, a chemical engineer with a Ph.D. — not at all qualified to make a judgment on Scott's abilities in the area
of «Approaches to Developing Serum - Free
Media Formulations for Mammalian
Cells in
Culture.»
To boost their odds, the researchers bathed the
cells in
culture media that encourage growth
of neural stem
cells.
After achieving 80 — 90 % confluency,
cell culture media were changed to maintenance
media with 2 % FBS and were inoculated with 200 µL
of clinical sample or 100 µL
of passaged viral supernatant.
Five days post-stimulation the anti-CD3 / anti-CD28 coated magnetic beads were removed from each
of the three
cultures (non-transduced (NTD), AdX4 - ZFNs, and AdR5 - ZFNs) and 2.5 million
cells were seeded in each
of four
cultures that were subsequently infected with either Bk132 (primary X4 isolate), HxB2 (lab - adapted X4 isolate), R3A (R5X4 primary isolate), or
media only (mock).
Efforts to promote a selective increase in the number
of endothelial
cells present in primary
cultures by using different
media preparations (MCDB - 131, D - Valine, etc.) were largely unsuccessful.
If the User requests isolated
cells, Users will provide an account number for a charge - back
of $ 100 for each hepatocyte / Kupffer
cell isolation, to cover the cost
of biochemicals, gradients and
cell culture media required for isolation
of purified
cell populations.
Consisting
of optimized
media and reagents, MimEX ™ Tissue Model Systems provides an alternative to current 3 - D
cell culture modes, such as organoids, with the added benefit
of...
StemFit
cell culture media has been independently evaluated by CGT Catapult, an independent centre
of excellence helping advance the UK
cell and gene therapy industry.
To generate your own edited clonal hiPS
cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media
cell lines, try one
of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single -
Cell Cloning DEF - CS Culture Media
Cell Cloning DEF - CS
Culture Media Kit:
Culture of Pig Induced Pluripotent Stem
Cells without Direct Feeder Contact in Serum Free
Media.
These
cells produced platelet - like particles (PLPs) similar to those isolated from human peripheral blood following an additional 18 days
of culture in a chemically defined
media without feeder
cells.
After addition
of exogenous IL - 2, the levels
of IFN - γ production in
culture media of stimulated CD8 + T
cells increased in all genotypes, again likely attributable to
cell number (Fig. 3F).
In these tests, StemFit not only delivered higher
cell proliferation, but also showed characteristics such as homogeneity
of gene expression compared with iPS
cells cultured with four other
media without any chromosomal abnormalities.
Hands - on Practical Training: Preparation
of Media and Capillaries, Sperm Collection and Cryopreservation, Superovulation, Oviduct and Uterus Flushing, Handling and
Culture of Preimplantation Embryos, In vitro Fertilisation, Controlled Freezing
of 2 -
cell embryos, Vasectomy, Plug Check
of Recipients, Surgical Oviduct and Uterus Embryo Transfer
PromoKine is a brand
of PromoCell, a premier manufacturer
of cell culture products which is especially recognized for its broad range
of human primary
cells, stem
cells, and blood
cells as well as optimized
cell culture media.
Expansion
of CD34 +
cells normalized relative to the values obtained in SFEM medium (dark gray bar) after
culturing purified CD34 + cord blood
cells for 7 days in StemSpan ™ serum -[SFEM, SFEM II (gold bar)-RSB- and animal component - free ACF (orange bar)
media, and six
media from other suppliers (light gray bars).
(A, B)
Culturing wild - type brains with conditioned
media from GBP1 and GBP2 expressing
cells induces low level
of ILP2 (A) and ILP5 (B) accumulation in the insulin - producing
cells.
(C, D)
Culturing wild - type brains with conditioned
media from GBP1 and GBP2 expressing
cells decreases the densities
of ILP2 (C) and ILP5 (D) signals in the insulin - producing
cells.
The automated
cell culturing and analysis system provides an optimal tool for the evaluation
of hESC
cultures, allowing continuous well - to - well comparison
of the effects
of different
culture media or different growth factor concentrations on
cell growth and behaviour — such as differentiation.
Significant advancements have been made in the bioprocessing
of hPS
cells including the formulation
of new
cell culture media, the development
of extracellular and acellular matrices that can replace feeder
cells, the development
of new enzymes for passaging and the discovery
of small molecules that significantly enhance the survival and clonogenicity
of dissociated
cells, allowing single
cell cryopreservation.
However, the industry appears to favor
cells adapted to suspension
cultures (e.g., HEK293), which can successfully grow in serum - free
media and thus provide a heightened safety profile and reduced cost
of goods (CoG)(33).
Culture media for tumor cells were supplemented with 10 % FBS, whereas culture medium for HUVEC was supplemented with 20 % FBS, with the exception of the experiment under low proliferation rate conditions (2
Culture media for tumor
cells were supplemented with 10 % FBS, whereas
culture medium for HUVEC was supplemented with 20 % FBS, with the exception of the experiment under low proliferation rate conditions (2
culture medium for HUVEC was supplemented with 20 % FBS, with the exception
of the experiment under low proliferation rate conditions (2 % FBS).
In addition to feeder free serum and LIF containing
media, ES
cells can be
cultured in minimal serum free
media (referred to as 2i) containing the MEK inhibitor PD0325901 that targets the phospho ERK branch
of the FGF pathway and the GSK3 - β antagonist CHIR99021 [42].
Additional wells containing
cells from the negative sort population were
cultured as above and after 17 days, pebble - like clusters
of small
cells were excised and transferred to ENStem - A neural progenitor
media (cat.
Briefly, NSCs (1 × 105 in 400 µL
culture media) were seeded into the upper chamber
of a 24 - well Boyden chamber with an 8 µm pore - size membrane, and tumor
cell - conditioned medium (600 µL) was added to the bottom chamber.
The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well
cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF)
media consisting
of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and
cultured at 37 °C in a 5 % CO2 incubator.
PromoCell is a premier manufacturer
of Human Stem & Blood
Cells, and Primary
Cells with optimized
cell culture media.
Effect
of stem
cell activation,
culture media of manipulated embryos, and site
of embryo transfer in the production
of F0 embryonic stem
cell mice
(A) Different biomolecules on the surface
of prepared silver nanoparticles (linker not shown); (B) in
cell culture media the nanoparticle - water interface is composed
of ligands and ions but also
of proteins, the so - called protein corona.
Influence
of semi-defined and defined
culture media on the development,
cell number and metabolism
of bovine embryos.
(E) Intracellular (internalized) and extracellular bacteria (
cell - free
culture media) were quantified at 1 h after ex vivo infection
of alveolar macrophages with an MOI
of 20.
The educational force
of the wider
culture is now the primary site where education takes place, what I have called public pedagogy — modes
of education largely produced, mediated, and circulated through a range
of educational spheres extending from the new
media and old broadcast
media to films, newspapers, television programs, cable TV,
cell phones, the Internet, and other commercial sites.
But in our current
culture, students get information from 400 channels
of television, the Internet,
cell phones, and a multitude
of other
media outlets.
ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and liver disorders) Band band
cell — type
of white blood
cell Baso basophil — type
of white blood
cell Bile Acids digestive acids produced in the liver and stored in the gall bladder (liver function) Bili bilirubin (bile pigment responsible for jaundice from liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and liver function) Bx biopsy C & S aerobic / anaerobic bacterial
culture and antibiotic sensitivity test (infection, drug selection) Ca +2 calcium ion — unbound calcium (parathyroid gland function) CBC - complete blood count (all circulating
cells) Chol cholesterol (liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image using injected radiopaque contrast
media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood
cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type
of white blood
cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake
of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (liver disorders) Glob globulin (liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup
of white blood
cells Hb, Hgb hemoglobin — iron rich protein bound to red blood
cells that carries oxygen (anemia, red
cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed -
cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type
of white blood
cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red
cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed -
cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part
of the C&S that determines antimicrobial selection Mono monocyte — type
of white blood
cell MRI magnetic resonance imaging (advanced tissue imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood
cell — immature red blood
cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed -
cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet —
cells involved in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood
cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood
cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type
of white blood
cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration, liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism, liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood
cell count (infection, inflammation, bone marrow suppression)
Helped design and carry out experimental procedures Performed Immunochemical staining Prepared
Media and aliquots Conducted basic biological manipulations
of bacteria Performed
cell culture and seeding Participated in the Department's Quality Control, Quality Assurance, Safety, and preventive maintenance programs.