Researchers believe it is the largest epigenetic study yet for any single cancer type and, importantly, the first to use a large cohort of primary patient tumor tissues instead
of cell lines grown in the laboratory.
The majority
of cell lines growing at the permissive temperature (33 °C) underwent an initial population doubling within 48 h.
Not exact matches
Where is the clear
line in a progression from (1) using animal insulin to treat diabetes, to (2) using gene remodeling techniques to
grow insulin in a host bacterium that will reproduce rapidly and from which a plentiful supply
of insulin can be harvested, to (3) genetic surgery to replace the defective gene in a person diagnosed as diabetic, to (4) genetic surgery immediately after fertilization in order to replace the defective gene and alter the germ
cells which would otherwise have transmitted the disease to one's offspring?
Capsaicin additionally produced a significant deceleration
of the development
of prostate tumors created simply by those human
cell lines grown in mouse models.
Newborns typically have loose junctures between the
cells of the stomach
lining, so during the first few months
of life, these junctures
grow tighter as your baby's protective tummy barrier matures.
Endometriosis occurs in women when
cells from the
lining of the womb
grown in other areas
of the body.
As well as allowing the use
of stem
cells grown from established
cell lines, the technology could enable the creation
of improved human tissue models for drug testing and potentially even purpose - built replacement organs.
For instance, a
growing number
of households no longer have land
lines, but rely on their
cell phones instead.
Introducing human prostate cancer
cell lines into mice, Wu and his colleagues saw a particular enzyme called MAOA activate a cascade
of signals that made it easier for tumor
cells to invade and
grow in bone.
To create the effect
of tobacco smoke on
cells, Vaz, Baylin and their colleagues began their studies with human bronchial
cells, which
line the airways
of the lungs, and
grew them in a laboratory.
In addition, where
cells derived from embryonic stem
cells are great at proliferating — a potentially critical feature if one wants to
grow sufficient numbers
of cells for clinical use — ones from the iPS
lines were much feebler.
Doing this in bacteria or
cell lines used for
growing chemicals would be
of huge importance to industry, where viral infections can shut down entire production
lines.
In a new study published in the journal Development, the KU Leuven researchers show that individual or small groups
of cells from uterus biopsies can be made to
grow into three - dimensional structures that show many
of the features
of the womb
lining, including the ability to produce mucus.
As other researchers asked for samples, the
cells replicated,
grew, and proliferated so wildly that they often took over and wiped out
cell lines of any kind with which they happened to come in contact.
«We believe an overwhelming number
of these altered
cells reach the
lining of the abdominal cavity, survive and
grow,» Bulun said.
The bacteria are full
of shiny white globules
of sulphur and
grow in long
lines of single
cells, making them look like a string
of pearls.
With the help
of my colleagues I started
growing retroviral vector - containing plasmids in bacterial cultures, isolating the plasmid DNA and transfecting special producer
cell lines with these plasmids.
Part
of the trouble is the ingredients: Subtle variations in tissue - culture chemicals and Matrigel, or in different stem
cell lines and how they are
grown first in 2 - D culture, can have a big impact on how the organoids turn out, Novitch says.
One idea is to
grow a
cell line from a single
cell removed from an early embryo, leaving the rest
of the embryo intact.
To test their hypothesis, the researchers carried out experiments on human melanoma
cells, a
line of cancer
cells they chose for their ability to
grow easily and quickly.
By functionally linking the signal transduction
of melanopsin to the control circuit
of the nuclear factor
of activated T
cells, we have designed a synthetic signaling cascade enabling light - inducible transgene expression in different
cell lines grown in culture or bioreactors or implanted into mice.
In studies
of laboratory -
grown human tumor
cell lines, the drug disrupted tumor
cell division and prevented growth
of advanced cancer
cells.
To do this, they switched from using dead tumour
cell samples to patient - derived tumour
cell lines, in which fresh samples
of a person's tumour are grafted onto mice and
grown to the required volumes.
The study used a well - known
line of pancreatic cancer
cells (AsPC - 1) in the laboratory and assessed how well this
grew when treated with either the chemotherapy drug gemcitabine or different levels
of commercially available chokeberry extract alone, and when treated with a combination
of gemcitabine and chokeberry extract.
Malaria parasites
grow in red blood
cells and stick to the endothelial
lining of blood vessels through a large family
of parasite proteins called PfEMP1.
All human
cell lines growing in tissue culture dishes, and about 95 %
of cells in a human being, split evenly, resulting in daughter
cells of equal size.
To find methods for stemming the tide
of liver - damaging microbes, Schnabl and team tried experimentally bumping up copies
of the REG3G gene in intestinal
lining cells grown in the lab.
For the time course study,
cells were treated with 20 μM
of EGCG for 12, 24, 48, 72, or 144 h. Human colon cancer
cell line HT - 29 and prostate cancer
cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were
grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.
NeuroStemcell is focused on the identification and systematic comparison
of progenitor
cell lines with the most favourable characteristics for mesDA and striatal GABAergic neuronal differentiation, generated either directly from human embryonic stem (ES)
cells, from Neural Stem (NS)
cells derived from ES
cells or fetal brain, from induced Pluripotent Stem (iPS)
cells or from in vitro short - term expanded neural progenitors from ventral midbrain
grown as neurospheres (VMN, Ventral Midbrain Neurospheres) 4, and perform rigorous and systematic testing
of the most prominent candidate
cells in appropriate animals models.
NCI's efforts to develop new laboratory models
of human cancer includes vastly increasing the number
of human cancer
cell lines (
grown as two - dimensional and three - dimensional cultures) and patient - derived tumor xenografts.
The Broad Cancer Dependency Map team adds CRISPR - based data from 342 cancer
cell lines to their
growing catalog
of genetic dependencies in cancer, and a new method for ensuring that data's accuracy.
The Joslin team found that intestinal tumors
grow just as quickly in a mouse cancer model whose «epithelial»
cells lining the inside
of the intestine have been genetically modified so that insulin can't activate the
cells.
The researchers established
cell lines from the original egg and in seven out
of eight cases the amount
of mutated mitochondrial DNA remained low when the
cells were
grown in the laboratory.
For the first time since the linkurl: National Institutes
of Health; http://www.nih.gov/ released its new guidelines for the derivation
of human embryonic stem
cell (hESC)
lines last summer, a
line approved under the Bush administration has been recommended for inclusion into the
growing federal registry
of lines eligible for federal funding.
Our study shows that selecting the appropriate
cell line,
growing the
cells as 3 - D aggregates and infecting them when they are fully differentiated is key for successful in vitro
cell culturing
of human noroviruses.»
This must be accomplished by using a process known as germ -
line transmission, where the primordial germ
cells (PGCs)
of an early embryo are edited,
grown in cultures, and then reproductively transmitted through surrogate host parents to generate live, engineered birds.
Since then, additional hESC
lines have been created (a «
line» consists
of all the
cells grown from one given blastocyst).
By
growing primordial germ
cells in culture researchers can make genome edits sequentially without being restricted by the breeding cycle
of living birds, and the cultured
cells will contribute to the germ
line when injected into new embryos.
In
line with these observations, all F958 - and P960 - targeting JAK1 mutations showed reduced inhibition
of STAT5 phosphorylation in response to CMP6, while all the other mutations had similar IC50 to that
of the parental BaF3
cells growing in the presence
of IL - 3 (data not shown).
As researchers look ahead to potential clinical trials with stem
cells, it is becoming apparent that few, if any,
of these
lines will have any therapeutic value, in part because they were
grown along with «feeder»
cells from mice.
There are companies that specialise in making
cell lines of certain genotypes that are sold for hundreds
of dollars, and different
cell types need different growth media and additives in order to
grow.
This
line of investigation could eventually lead to artificial blood vessels,
grown with patients» own
cells or «off the shelf,» or biological / pharmaceutical treatments that promote the regeneration
of damaged blood vessels.
(The first
line of human
cells to survive in vitro is the so - called HeLa
cells,
grown in 1951 — it's still the most commonly used
cell line for human
cell research.)
Stem
cell lines grown in the lab provide scientists with the opportunity to «engineer» them for use in transplantation or treatment
of diseases.
The lack
of consent and information are one side, but in addition to being an invaluable research tool,
cell lines are also big business: The global market for
cell lines development (which includes
cell lines and the media they
grow in, and other reagents) is worth around 3 billion dollars, and it's
growing fast.
When injected into immunodeficient mice, the iPS
cells grew into neural tissue, cartilage, muscle, and forebears
of cells that
line the intestinal tract — representing all classes
of tissue that give rise to a complete body.
Researchers have
grown and used around twenty tons
of her
cells and research relating to this
cell line has generated seventy - six thousand abstracts on Pub Med.11
Meat and fish stocks provide building blocks for the rapidly
growing cells of the gut
lining and they have a soothing effect on any areas
of inflammation in the gut.
But so great is this culture's obsession with her that Syd has developed a nice little side -
line peddling her infections on the black market to a man who, by day, sells celebrity steaks
grown from the
cells of the star
of your choosing.
In the body, this virus attacks and
grows within the white
cells (lymphocytes and macrophages)
of the blood and lymphatic system as well as the
cells that
line the intestinal tract.