Sentences with phrase «of cell pellets»

Crude genomic DNA extracts (gDNA) of 78 AID - negative Amrose subclones were prepared by incubation of cell pellets of app.

The laboratory also offers frozen sectioning, special staining, processing of cell pellets, alcohol fixation, DNA / RNA and LCM preparation and sectioning, and consultations on research protocols.

Not exact matches

To make its expanded polystyrene trays, Commodore purchases plastic pellets that are heated and injected with gas to form cells that are then made into rolls of foam.

Perigonadal adipose tissue was collected from female B6.V Lepob / ob mice, digested, and centrifuged to yield a buoyant adipocyte - enriched cell population (gray bars) and a pellet of SVCs.

Pellet cells at 180 × g for 5 min, wash once with 4 ml FACS buffer, and pellet cells at 180 × g for 5 min (if staining high amount of cells perform another Pellet cells at 180 × g for 5 min, wash once with 4 ml FACS buffer, and pellet cells at 180 × g for 5 min (if staining high amount of cells perform another pellet cells at 180 × g for 5 min (if staining high amount of cells perform another wash).

Cell supernatants were discarded and cell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analyCell supernatants were discarded and cell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analycell pellets were lysed of RBCs and then used for staining with antibodies for flow cytometry analysis.

Approximately one million cells of each clone were inserted into 0.9 mm capillary tubes and centrifuged to form a small pellet; this amount corresponds to a small primary tumor, or average size metastasis.

Modality of access under this proposal: Users can provide human or animal serum, plasma or tissue samples, or cell pellets or supernatants from cell models.

ChIP for a set of 7 histone modificiations (H3K4me1, H3K4me3, H4K9me3, H3K27me3, H3K27ac, H3K36me3 and H3K79me2) for one cell pellet.

Chromatin preparation and shearing from single frozen pellet of cells cross-linked with formaldehyde.

With regard to cell - type variation, we examine variation in concentration of cell - types in lymphocyte pellets to identify variation that may reflect relatively greater responsiveness of the innate vs. the acquired immune system.

Ficoll purified peripheral blood mononuclear cell pellets of the sort used in the current investigation are comprised of several different cell types (e.g., CD4, CD8, CD14, CD19, CD56)(Reinius et al., 2012).