Sentences with phrase «of cell surface marker»

Single cell Q - RTPCR for the genes indicated was carried out on cells separated by flow cytometry on the basis of cell surface marker expression.

Lipke, who specializes in the structure and function of cell surface markers in yeasts, says Rezende was as independent as a typical late - term Ph.D. student.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

In breast cancer, CSCs or tumor - initiating cells were first identified by using a combination of cell surface markers, CD24 − / CD44 + / ESA (EpCAM) + (2).

Phenotypic analyses of cell surface markers in spleen, lymph node, and thymus were performed on unchallenged mice at 6 — 8 wk of age.

Lineage - restricted progenitors are identifiable by expression of distinct combinations of cell surface markers.

ES cells can be described based on a characteristic morphology, the presence of cell surface markers such as SSEA - 1 and Pecam1, or the expression of the key transcription factors such as Oct4, Sox2, Nanog, and a number of ES cell - specific transcripts (ECATs)[4]--[6].

We derived a fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize the expression of cell surface markers commonly expressed on pluripotent stem cells (Figure 1B, C and D).

A. Fractionation of HES 3 or H9 cells by flow cytometry according to the levels of expression of cell surface markers (GCTM - 2, pericellular matrix proteoglycan, and CD9).

Not exact matches

Weeks later, Yee realized that he didn't have the equipment he needed to pluck out of Ziskin's blood the rare (perhaps one in 100,000) T cells that could identify the subtle peptide markers on the surface of her cancer cells and attack the disease.

Several studies have used cell - surface markers — proteins found on the outer membranes of tumor cells — to identify glioblastoma stem cells; but the specific markers used have been controversial and can not reflect molecular processes going on within tumor cells.

They also showed that the cells have most of the surface «markers» considered as identifiers of embryonic stem cells and form «embryoid bodies» — characteristic clumps of cells formed by ESCs.

Researchers from BUSM and the University of Cyprus compared the markers on the surface of the cancer cells to gene expression profile of breast tumors deposited by researchers in international public databases and found that a molecule named IL13RA2 (IL13R alpha2) was abundant in metastatic or late - stage BLBC.

To prove this point, he and Saito applied their miRNA switches to the purification of hepatocytes and pancreatic cells, neither of which has unique cell surface markers, finding miRNA switches effective there too.

At this time, it is only possible to verify the decision retrospectively with cell surface markers,» explains Dr. Carsten Marr, head of the Quantitative Single Cell Dynamics Research Group at the Helmholtz Zentrum München's Institute of Computational Biology (Icell surface markers,» explains Dr. Carsten Marr, head of the Quantitative Single Cell Dynamics Research Group at the Helmholtz Zentrum München's Institute of Computational Biology (ICell Dynamics Research Group at the Helmholtz Zentrum München's Institute of Computational Biology (ICB).

A stem cell is generally characterized by the presence of various proteins — or cell markers — on its outer surface.

Comparing the markers on these stem cells with those on the surfaces of cancer cells, Johnson adds, will also help scientists determine if stem cells contribute to prostate malignancies.

«We've not had cell surface markers for the different stem cell states before, which has made it hard to study them,» says Fredrik Lanner, assistant professor at Karolinska Institutet's Department of Clinical Science, Intervention and Technology.

The Discher Lab has since shown that a protein on human cells called CD47 functions as a «marker of self» by interacting with a protein on the surface of macrophages called SIRPA.

«Only a subset produces IL - 17A upon skin injury, although the surface markers on these cells seem identical to those of other DETCs,» MacLeod said.

The cancer cell marker that Johnson and her team identified was a specific change in protein glycosylation, that is, a unique pattern of sugars decorating a protein found on the cell surface.

F3 can detect the expression of a protein called nucleolin, which is a marker on the surface of tumor cells.

Cancer cells settle to the bottom of the well, where they selectively bind to the antibodies based on their surface markers (unlike other devices, the chip can also trap tiny structures called exosomes produced by cancers cells).

Each well holds a specific antibody that will bind selectively to one type of cancer cell type, based on genetic markers on its surface.

Significantly, cells with reduced mtDNA became self - renewing and expressed specific cell surface markers characteristic of breast cancer stem cells.

Wang explained that, because ECMs are composed of collagen, elastin, carbohydrates and signaling molecules and have no cell surface markers, DNA or RNA from the donor, the recipient is less likely to reject the treatment.

We will develop mathematical models to predict the pathways of differentiation from naive to memory and effector T - cell subsets based on the characterisation of surface marker expression, transcription factors and cytokines production at early and late time points after immunisation.

HLA (human leukocyte antigen)- markers on the surface of a body's cell that identify the cell as one's own (as opposed to a foreign cell) and prevent the immune system from attacking itself.

Further research uncovered a broad spectrum of cell surface stem cell markers (e.g., CD133, CD44, and CD24) that allow the identification of CSCs in human solid tumors, including brain, breast, prostate, pancreas, liver, ovary, skin, colon cancers, and melanoma (3 - 6)(Figure 1 based on 7).

To compare T cell phenotypes between DGKζ − / − and Cbl - b − / − mice, and to evaluate DKO mice, we isolated thymi, lymph nodes, and spleens from mice of each genotype and determined expression of T cell surface markers.

Differential expression of surface markers in mouse bone marrow mesenchymal stromal cell subpopulations with distinct lineage commitment.

Purified cells were surface stained for a variety of leukocyte markers and analyzed using flow cytometry.

Interestingly, an increased fraction of the cell population was found to express CD44 + / CD24 - / ESA + cell surface markers.

Since very few studies have demonstrated the formation of macro-metastasis from low numbers of sorted CSCs and currently proposed CSC markers might not be sufficient to identify all stem cell populations [13], we believe that our study presents a promising approach to isolate stem - like and metastatic breast CSCs as opposed to the cell - sorting strategy based on putative stem cell surface markers.

To characterize activation of immune cells, measurement of intracellular signaling using phospho - specific antibodies combined with immune phenotyping using surface markers is ideal.

Luckily, different cell types tend to have different things on their surfaces, which play particular parts in their specialized roles in the tissue, so it is a matter of identifying and targeting cell - surface markers that are specific to these abnormal cell types.

By means of a non-obese diabetic / severe combined immunodeficiency disease (NOD / SCID) xenotransplant assay in combination with specific cell surface markers (CD44 + CD24 - / low), CSCs were enriched from metastatic and primary breast tumors and were shown to have the ability to reestablish tumor heterogeneity after transplantation [1].

Positive clones were then differentiated using defined serum - free conditions outlined in Ng, et al [7], which overall gave rise to a CD45 + CD56 + CD117 − CD94 + population of cytotoxic NK cells [8] expressing the CD4 construct and other surface markers in a similar manner to peripheral blood NK cells.

These studies led to identification of a cell surface protein that is a useful marker of positive selection and is a likely regulator of mature lymphocyte and APC function.

g defined serum - free conditions outlined in Ng, et al [7], which overall gave rise to a CD45 + CD56 + CD117 − CD94 + population of cytotoxic NK cells [8] expressing the CD4 construct and other surface markers in a similar manner to peripheral blood NK cells.

Here we consider two surface markers thought to define these cells in mice, CD8 and Killer cell lectin - like receptor G1 (KLRG1), and a means we developed to remove these cells from the blood of aged C57BL / 6 mice.

However, SSEA1 can be a useful cell surface marker for the detection and enrichment of putative germ cells derived from ES cells as reported previously [56].

(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.

Monoclonal antibodies are a special type of protein designed to target antigens, or markers, located on the surface of cancer cells; antibodies locate antigens and recruit immune cells to attack.

These cells often have virus - specific T cell receptors, as well as other surface markers that distinguish them from their youthful counterparts, and they are thought to play a major role in the decline of the immune system with age.

An inherent problem with this approach is that the presence of specific cell surface markers does not directly reflect the transcriptional state of a cell.

Most of these CSCs have been identified using surface markers that identify their corresponding normal tissue stem and progenitor cells, thus reinforcing the resemblance of tumor development to normal organogenesis.

In the present study, we again observed a continuous gradient in the expression of pluripotency genes across the cell populations that paralleled the gradient in cell surface marker expression.

(B) Percent of sorted single HES3 cells in (A) expressing stem or lineage markers according to level of surface marker expression.

People who had a better antibody response to the vaccine after 28 days had higher levels of HLA - DR (a cell surface protein which is a marker for immune stimulation) on a specialised type of dendritic cell.