Sentences with phrase «of cellular phenotypes»

The focus of the Mar lab is to understand how variability in gene expression contributes to the regulation of cellular phenotypes.

Not exact matches

The objective of the research project led by Marko Kallio, Principal Scientist at VTT, was to accelerate the drug development process by identifying new compounds that would possess similar binding properties and cellular phenotype, but a different chemical structure, as the selected drugs in clinical use or investigational compounds in development.

Using this approach, researchers can easily generate diseaserelevant isogenic models to determine the impact of correcting or introducing disease - relevant mutations on cellular phenotypes in a tissue - appropriate context.

He also envisions that this kind of nanomechanical phenotyping should allow for the development of predictive models for cellular behavior for any kind of cell.

Environmental factors interact with the different subgenomes to modify the transcription of their component genes and to modulate the translation of protein products and their posttranslational modification, yielding changes in protein and cellular function and metabolism, and defining an intermediate phenotype.

This series addresses the contribution of cellular senescence to cardiovascular, neurodegenerative, and arthritic disorders as well as the senescent phenotypes in various tissues and cell types.

Often this garners more compelling evidence of a gene's importance, because it demonstrates the relationship between a genetic entity and phenotype visible at the cellular level or above.

In order to dissect these defects, we utilize a unique approach including deep assessment of patient phenotype, clinical genetic testing to identify underlying genetic contributions to these features, and the application of combinatorial functional omics (transcriptome, metabolome, etc.) to uncover the pathological cellular states that result from these genetic changes.

The ability to isolate pure CSC populations using cellular markers has led to advances in our understanding of the heterogeneity and plasticity of the CSC phenotype.

Here, Anna presents how studies of neural stem cells and neurons derived from iPS cells of patients show faithful mimicking of known disease phenotypes in our cellular models of disease, like Alzheimer's disease, autism, and Down syndrome.

RNAi - mediated silencing of RASSF1A induced epithelial - to - mesenchymal transition (EMT), fomenting a motile and invasive cellular phenotype in vitro and increased metastatic prowess in vivo.

The consequences of gene extinction on DM1 phenotype is then monitored at the molecular level by quantitative PCR or at the cellular level by high content screening in 96 or 384 - well plate format.

Employing a combination of cellular, biochemical and genetic experiments, we showed that (i) human and murine pericytes express functional Tie2 receptor, (ii) Tie2 - silenced pericytes have a pro-migratory phenotype, (iii) Tie2 downstream signalling in pericytes involves Calpain, Akt and FOXO3A, (iv) Ng2 - Cre - driven deletion of pericyte - expressed Tie2 delays developmental angiogenesis and vessel maturation, and (v) Tie2 deletion in pericytes results in a pro-angiogenic tumour vasculature with enhanced tumour growth.

Current explorations into the molecular underpinnings of cell circuitry are leveraging multiple data types including expression profiling and epigenetic analysis leveraging RNA - seq and CHiP - Seq coupled with molecular markers and cellular phenotypes.

The apparent temporally identical and phenotypically opposing tumour vessel phenotypes may relate to the cellular findings of the present study, in which the pericyte Tie2 loss - of - function phenotype phenocopied the Ang2 gain of function.

She has discovered several novel disease specific cellular phenotypes in their in vitro models, many of them involved in accurate performance of the neural progenitors.

(9) Indeed, the degenerative aging process is by definition one in which the organism progressively accumulates damage to its cellular and molecular components over time, so any genetic or environmental factor that leads to a greater burden of such damage will bear some resemblance to the aging phenotype, irrespective of the causal origin of the defect or its relationship to «normal» aging.

The SMDC has allowed Gladstone scientists to design molecules to modulate the functions of proteins or specific cellular phenotypes and, most significantly, screen for drugs that could be used as therapies for disease.

The routine use of MSC cellular therapy with would benefit from a uniform cell source, with stable phenotype and function as well as providing a solution to potential safety issues.

By expanding high - throughput deep phenotyping of cells beyond protein epitopes to include RNA expression, PLAYR opens a new avenue for the characterization of cellular metabolism.

Therefore, this work provides strong support for the use of iPSC ‐ derived MSC as a more uniform with well ‐ defined phenotype and function as a sustainable source of cells which paves the way for a clinical application for IBD cellular therapy.

To define the phenotype and function of specific cellular populations involved in the cross-talk between cancer and immune cells we make use of both flow - cytometry and mass - cytometry.

These data indicate that cellular senescence is causally implicated in generating age - related phenotypes and that removal of senescent cells can prevent or delay tissue dysfunction and extend healthspan.

Only in the last 10 years, with increasing knowledge of the senescent phenotype and the ability to detect senescent cells in human tissues, have biologists been able to investigate the relationship between cellular senescence and disease.

This lack of understanding is contributed by the fact that cellular aging is a complex phenotype to measure and comprehensive studies on aging require the application of novel experimental approaches and technological platforms.

Genome - wide CRISPR - based knockout (CRISPR - KO) screening is an emerging technique which enables systematic genetic analysis of a cellular or molecular phenotype in question.

Through genome - wide profiling we find that 5 - 46 % of the variation in different iPS cell phenotypes, including differentiation capacity and cellular morphology, arises from differences between individuals.

Children suffering from autism have been reported to have low bone mineral density and increased risk for fracture, yet the cellular origin of the bone phenotype remains unknown.

Other flow cytometry applications include: cellular phenotyping by multiparameter cytofluorimetric analysis, sorting of fluorescence protein - tagged cells, assessing apoptosis and cell cycle status, the identification / isolation of stem and cancer stem cells (e.g., marker positive, side - population, etc.) and the deposition of single cells.

This study therefore characterizes the structural and cellular bone phenotype in a mouse model of autism that can be further utilized to investigate therapeutic avenues to treat bone fractures in children with autism.