It is unclear whether components
of the culture medium other than extrinsic factors affect germ cell differentiation in vitro in monkeys.
The observation that SCP1 was expressed in EBs even without extrinsic factors (Figure 5B) may suggest that the components
of the culture medium are also one of important factors to effectively induce germ cell differentiation, especially meiotic progression in vitro.
Confirmation that the visualized spirochete was B. burgdorferi was done by PCR on a sample
of the culture medium, as reported elsewhere [25].
One - half
of the culture medium was replaced with a fresh medium every 3 — 4 days for the 16 DIV.
To find out, they put droplets
of culture medium that cancer cells had grown in on one side of petri dishes.
«When someone has a problem — whether they need to find a particular cell line or need to understand the formulation of a particular type
of culture medium — they're coming to you and they're in a crisis.
Not exact matches
Such is the appeal — and the economic power —
of gaming, a
medium whose shadow has expanded to eclipse music, movies, and television on the pop
culture landscape.
On June 30, star investor Chris Sacca wrote on
Medium that he took some personal responsibility for «the unrelenting, day - to - day
culture of dismissiveness that creates a continually bleak environment for women and other underrepresented groups» in Silicon Valley (shortly before allegations
of his own sexual misconduct emerged in the same piece that broke the story about McClure).
«We are disappointed to see that Uber has selected a team
of insiders to investigate its destructive
culture and make recommendations for change,» they wrote in an
Medium post published on Thursday, referring to the fact that Holder's firm has close ties to Uber and he previously advocated against fingerprint - based background checks for Uber drivers.
Some reports, such as the Open Philanthropy Project's Animal Product Alternatives report and Van der Weele & Tramper (2014) suggest it is unlikely that
cultured meats will become cost - competitive with conventional meat.98, 99 One important contributing factor in this conclusion, which is cited in these reports, is the minimum costs
of the growth
medium necessary for
culturing the desired cells.
In 2017 alone, Entrepreneur Magazine ranked us on the list
of Best Company
Cultures, Austin Business Journal voted us 8th Best Place to Work (
Medium Category), and we won a Stevie Award for Great Employers.
What I'd like us all to take from «wm» is a conviction that conservative pop
culture studies have to attend to the impact
of the
medium as well as
of the message, or better said, since McLuhan can't be fully right, that we must grapple with the popularity as well as the
culture of pop
culture.
Hyderabad, under the Muslim dynasty
of the Nizams, had become a center
of Muslim
culture and Urdu literature and poetry after the establishment
of Usmania University, the first institution to use Urdu as the
medium of instruction in higher education.
The message behind the «
culture creep» promoted by the Times is a «gospel,» says veteran reporter Proctor, because it is adhered to with religious - like devotion both in the editorial and news sections
of that influential
medium.
Nevertheless, it was also the «sole
medium of intellectual life» and could become again, incidentally to the process and quite unintentionally, a way
of access to the heritage
of pre-Christian Roman
culture and classical Latin literature (Erich Auerbach, Literary Language and Its Public in Late Latin Antiquity and in the Middle Ages [Pantheon.
Those souls who would grow must either transplant themselves or overcome the poison in the very
medium of their
culture.
With some entailment
of that danger always implicit in superlatives, one may raise the question whether any other single contribution from whatever source since human
culture emerged from the stone ages has had the far - reaching effect upon history that Israel in this regard has exerted both through the
mediums of Christianity and Islam and directly through the world
of Jewish thinkers themselves.
(29) Because language is a primary
medium of communication, its very structure is affected by social organization and
culture.
For years, the prestige TV movement has helped the
medium of serialized storytelling become pop
culture's most important channel for engaging big ideas and sparking social dialogue the way novels...
True — I blame the over-sexualized
culture we live in which is a direct by - product
of the growth
of the
medium of advertising and the sexual revolution.
The school stands as the only mass
medium capable
of putting forward the case for what is not happening in the
culture.
At the same time video can return the emphasis
of theology to story, although we really have no idea yet how this universal electronic - image
medium is going to shape theology and
culture.
And it must attend to the languages that are the
medium of public discourse in congregations» host
cultures.
So it is a matter
of plain fact that Christianity molded what came to be called Europe (whose original name, after all, was «Christendom»), but to say so does not by itself tell us whether that shaping
of European
culture through the
medium of Christian ideas was a good thing or a bad thing to begin with, let alone whether those ideas speak to us now.
In this view,
culture is not an enemy, but a
medium in which unsaved people are trapped, or have trapped themselves (sort
of a tar - baby situation).
The objective
of the Islamic slaughter is to ensure maximal blood drainage from the animal as Muslim belief blood is a good
culture medium for microorganisms to flourish.
* 3/4 cup crème fraîche (I used my homemade version) * 3 - 4 tablespoons raw apple cider vinegar * 2 tablespoons
cultured buttermilk * 1 handful
of fresh basil leaves * 1 handful
of fresh cilantro leaves * 1 handful
of fresh parsley leaves * 2
medium cloves
of garlic, sliced in half, plus more to taste (if you love garlic) * pinch
of ground cayenne pepper — optional * sea salt and freshly ground pepper to taste
Ghee rendered from
cultured butter is Chef Tory Miller's frying
medium of choice for the fingerling potato chips that go with his beef tartare: «The chips get super crispy, with that buttery funkiness,» says Miller, the chef at Graze, an eclectic gastropub in Madison, Wisconsin.
Kombucha, which many
of you are likely familiar with, differs from water kefir because kombucha is specifically
cultured in a tea and sugar
medium, and water kefir can be
cultured using just water and sugar.
Using the format
of the cookbook as a storytelling
medium, the In Vitro Meat Cookbook is a visually stunning exploration
of the new «food
cultures» lab - grown meat might create.
You can use it as a
medium to introduce many concepts
of science, environment,
culture and etiquettes very early on in their life.
The fertilised egg (zygote)
cultured for 2 - 6 days in a growth
medium and is then transferred to the mother's uterus with the intention
of establishing a successful pregnancy.
When the scientists succeeded in collecting the queen, part
of the colony was transported to the laboratory, and all the bacteria found on the insects» surface and inside their bodies were isolated, characterized, and placed in
culture medium.
When glucose is added to the
culture medium for cows and sheep embryos, a greater number
of males survive.
Although electrolyte leakage is still undesired, its danger is minimized by the use
of either the normal saline solution pumped into the body in most IV treatments or a cell -
culture medium that contains amino acids, sugars, and vitamins in addition to sodium ions and thus mimics the fluid that surrounds human cells.
Previous work by the researchers developed plasma - activated cell
culture medium as a form
of chemotherapy, but selected Ringer's solution in the present work because
of its simpler composition and likelihood
of forming less complex reaction products.
To shock the plants, Cuello applied a 30 to 100 milliamp current to the growth -
medium of plants grown hydroponically, or, in the case
of barrel medic, to the solution surrounding the cell
cultures.
Their stem cells, which can mature into any type
of tissue, were isolated and
cultured in a dish around dots
of gel - like growth
medium.
Following cultivation
of Arabidopsis seedlings on a
culture medium containing a high concentration
of cesium carbonate, Cesium Green was applied to the seedlings, and the resulting green fluorescence observed was used to confirm the presence
of cesium within the plants» cells.
The resulting organoids could either be banked for future use or immediately fragmented and seeded into the «epithelial» channels
of multiple Organ Chips where they were further matured by flowing specialized
culture medium and applying mechanical stimulation to the channels to mimic physiological peristalsis - like motions.
One reason for the persistence
of this pyramid
of production is that for the past half - century, much
of the world's media
culture has been defined by a single
medium — television — and television is defined by downloading.
To confirm that the phosphate was biological in origin, the German researchers added human faeces to a
culture medium and then incubated the samples under anaerobic conditions at a temperature
of 36 °C.
«Spinal fluid is a great
culture medium — one
of the best,» he says.
The cells were
cultured in a suitable
medium and the researchers checked the molecules they produced, in particular the pro-angiogenic ones, that is, molecules that nurture the production
of new blood vessels.
Maures et al. (p. 541, published online 29 November) explored why the presence
of abundant mating - competent males causes a decrease in the life span
of nematodes
of the opposite sex and found that a secreted substance, possibly a pheromone, reproduced the effect
of the males when transferred in the
culture medium.
Embryo growth after 12 months
of dry storage was negligible and was observed only in embryo
cultures with growth hormones (c. 12 %) and no embryo growth was evident after 3 months
of storage at − 18 °C in either
medium.
Cultures were maintained in complete
medium consisting
of Neurobasal
medium (Invitrogen, Carlsbad, CA) supplemented with B - 27 (Invitrogen), 1 % FBS (Hyclone, Logan, UT), 0.4 mm l - glutamine (Invitrogen), 2.5 gm / l glucose, and 10 ng / ml 2.5 S nerve growth factor (Becton Dickinson, Bedford, MA).
The addition
of a small amount
of venom to the
culture medium in the absence
of the extract
of sarcoma 180 elicited the growth
of the same dense halo
of nerve fibers around an isolated sensory or sympathetic ganglion.
Sensory and sympathetic ganglia were dissected out
of eight - day - old chick embryos and
cultured in a semisolid
medium in proximity to fragments
of mouse sarcoma - 180 tumors.
The growth potential
of excised embryos
cultured on 1/2 MS
medium declined by 31 % after 1 month
of dry storage or after 24 h
of dry storage at − 18 °C (Table 3), but growth was similar to that
of embryos from freshly collected seeds (c. 80 %) when embryos were
cultured with growth hormones.