Sentences with phrase «of different assays»

Systems will become faster and more efficient and be able to run a larger variety of different assays.

Not exact matches

The newly proposed Comprehensive in Vitro Proarrhythmia Assay (CiPA) offers a different strategy for defining proarrhythmic risk based upon a clear understanding of torsadogenic mechanisms beyond simply blocking hERG / IKr current.

The researchers measured toxicity using a biological assay, by counting the number of dead Daphnia — tiny crustaceans — in water containing the different ladybird toxins.

In a different set of experiments, the food chemists tinkered with baking conditions and then ran five different test - tube assays of the crust's antioxidant activity — its ability to quash free radicals.

«We found the type of assay gives different results.

It is great that we can combine the results of different techniques, chemical analytics, electrophysiological measurements, imaging methods and last but not least behavioral assays.

Professor Dianna Bowles, a plant biochemist and founding Director of CNAP, led the research with Professor Paul Kaye, the Director of CII, who developed the robust assay system involving human cells to assess the impacts of the different structures.

The study compared the main different ways of diagnosing C - Diff, including the two «gold - standard» methods: a cytotoxin assay, which looks for the presence of C - Diff toxin in faecal samples; and cytotoxigenic culture, which looks to see if there are bugs present in faecal samples that could possibly produce C - Diff toxin.

Measurements were performed with different microgel barcodes and one in particular focused on specific microRNA targets, demonstrating the specificity of the assay for multiplex measurement conditions.

By performing different biochemical assays, the researchers observed that PLK4 is capable of auto - regulating its levels by adding chemical groups of phosphate to itself, which will act as a signal for destruction.

«This new generation of integrated device design makes it possible for centrifugation - free sorting of different blood components, which can drastically reduce the cost and processing time involved with liquid biopsy assays.»

To determine the effects of the loss of IL - 15Rα on exercise performance in vivo, we quantified activity of IL - 15Rα — KO mice and B6129 control mice using two different assays: cage wheel running and quantification of photobeam breaks.

By establishing an assay system where free virus infection is restricted and infection can only occur through cell - cell transmission, the researchers could then test whether a large selection of Nabs could prevent cell - to - cell transmission of different HIV strains.

In the context of the «European Training Programme in Vaccinology «Fraunhofer IME will provide three different Training Modules including Assay Development and Validation, focused on SPR technology, Process Development and scale up, as well as on Requirements for GMP - production.

Once developed, the assays will be used to analyse in vitro cytokine and chemokine induction, e.g. to compare different versions of a vaccine, or the same vaccine produced in different host cells, or different batches of a vaccine to demonstrate batch - to - batch reproducibility.

This will include testing the mechanism of action of different adjuvant and antigen formulations by measuring B - and T - cell responses and by using various functional assays (ELISA, FACS, cytokine profiles), transcriptomics and / or proteomics.

«By getting me involved in the project, not only could we do these biological, cell - based functional assays that Roy's lab carries out, but we could also purify the integrins or parts of (them) and do both structural and biophysical characterization of interactions — how the different subunits stick together,» he said.

I completed my PhD as a cell biology major in an engineering lab, where I established a new assay to answer questions very different from what the main part of the lab was working on.

After 24 h of irradiation, amount of cell death in different groups was measured using TUNEL assay (EMD Millipore, Cat.

Evaluation of the Aptima HIV - 1 Quant Dx Assay for HIV - 1 RNA Quantitation in Different Biological Specimen Types.

Overall a total of 1969 pools of mosquitoes collected at different sites in Kedougou between May to December 2011 were tested using the ZIKV rRT - PCR assay.

iTeos uses a platform of in vitro and ex vivo screening assays to reproduce immunosuppression induced by different components of the tumor microenvironment.

Guideline to aid the design and validation of clinical CYP2C19 assays and promote standardization of testing across different laboratories.

This allows for a one - step PCR reaction and pooling of amplicons from several different samples and targets (generated with other LymphoTrack Assays for the Illumina ® MiSeq ® instrument, sold separately), onto one MiSeq ® flow cell allowing for up to 24 samples per target to be analyzed in parallel in a single run.

We currently evaluate the therapeutic benefits as well as the toxicity of drug candidates in spheroids by means of conventional fluorescence microscopy as well as LSFM and many different biochemical and molecular biology - based assays (Ansari et al. 2013; Wenzel et al. 2014; Pampaloni & Stelzer 2010).

5xFAD mice at three different ages were compared to wild type littermates in regard to metabolic parameters and enzymes of the gut by... fluorimetric enzyme assay and western blotting.

(B and C) Western blot and immunofluoresence assay of Jurkat cells transfected with wild - type (WT), KMT2D mutants (V5486M)(B) and EP300 mutants (H1377R)(C) upon treatment with different HDAC inhibitors.

The overall score encompass several factors: reproducibility of the antibody staining in different cell lines (and if signal strength correlates with RNA expression levels); assays for enhanced antibody validation by using antibodies binding to different epitopes on the same target protein (independent antibodies), by knockdown / knockout of the target protein (genetic methods) and by matching of the signal with a GFP - tagged protein (recombinant expression); experimental evidence for location described in literature.

and affinity of antibody ACI - 5400 were characterized by a panel of methods: (i) measuring the selectivity for a specific phospho - Tau epitope known to be associated with tauopathy, (ii) performing a combination of peptide and protein binding assays, (iii) staining of brain sections from mouse preclinical tauopathy models and from human subjects representing six different tauopathies, and (iv) evaluating the selective binding to pathological epitopes on extracts from tauopathy brains in non-denaturing sandwich assays.

Testing of 51 specimens from CFS patients and 56 matched and healthy controls from the US was performed independently in three laboratories for XMRV DNA by using several PCR tests and for anti-XMRV antibodies using different serological assays.

Four different stages of oocytes, striped of their zona pellucida (ZP), were assayed: 1) GV (germinal vesicle), 2) MI (metaphase I), 3) MII (spontaneously matured metaphase II before IVM) and 4) IVM - MII (in vitro matured metaphase II).

Different in vitro assays were carried out in order to test the specific effects of kahweol treatment on several key steps of the angiogenic process in both endothelial and tumor cells.

The gelatinolytic assays were carried out in two different ways to obtain complementary information: firstly, cells were treated or not with the test compound and samples from these were submitted to gelatinase zymography to detect the effects of the kahweol treatment on the expression of gelatinase activities; secondly, in some experiments, samples from control, untreated HT - 1080 fibrosarcoma cells were submitted to zymography and, after electrophoresis, different concentrations of kahweol were added to the substrate buffer to determine the potential direct effect of kahweol on gelatinase activity.

Data are given as percentage, taking the normalized levels of COX - 2 in control cells as 100 %, and they are means ± S.D. of three different assays.

Design of strategies for integrating different sources of information (e.g., different assay results) in biomedical studies

For all adhesion assays two different concentrations of each matrix (Fibronectin; 74 ng / cm2 and 222 ng / cm2, Collagen I; 37.4 ng / cm2 and 112 ng / cm2, Collagen IV; 111.11 ng / cm2 and 333.33 ng / cm2 and Optimat which is mixture of these matrices with combined final concentration of 222.5 ng / cm2 and 667.6 ng / cm2) were coated by incubating at 37 °C for overnight.

XMRV was not detected in a new set of 39 prospectively collected prostate tumors (both with or without RNAse L mutations) by PCR assays performed independently in 3 different laboratories or ViroChip microarray.

Three different research institutes (Blood Systems Research Institute, Abbott Laboratories, and Cleveland Clinic) performed independent PCR - based NAT assays for the gag, pol, or env genes of XMRV, respectively.

(A) EC50 values from MTT assay using silver nanoparticles with different coatings and HepG2 cells; (B) Analogous with Neuro - 2 cells; (C) Detection of oxidative stress from Ag - NPs (concentration 5 pM) via formation of protein carbonyls incubated with HepG2 cells.

Additional experiments using RNA isolated from stocks of viruses from different virus families showed no cross reactivity of the assay with influenza A virus, influenza B virus, rhinovirus, human coronaviruses 229E, OC43, and NL63, and adenovirus (data not shown).

This suggests that our automatic screening assay is specifically measuring the effect amiodarone HCL has on different populations of cells.

The team's latest AR model integrates the results of 11 high - throughput in vitro assays, each targeting a different part of the AR pathway.

Assay, sample and side - to - side variation were quantified by analysis of variance and presumptively incorrect decisions related to high - risk disease were determined using the result from each MRD assay, the mean MRD in the patient as the measure of the true value, and each of 3 different MRD cut - off levels which have been used for making decisions on treatAssay, sample and side - to - side variation were quantified by analysis of variance and presumptively incorrect decisions related to high - risk disease were determined using the result from each MRD assay, the mean MRD in the patient as the measure of the true value, and each of 3 different MRD cut - off levels which have been used for making decisions on treatassay, the mean MRD in the patient as the measure of the true value, and each of 3 different MRD cut - off levels which have been used for making decisions on treatment.

This is the first study demonstrating smoking - induced expression changes for this particular set of genes and importantly, it is the first time partial reversibility (MUC5AC) and irreversibility (GSK3B) and has been demonstrated using two different cohorts of samples with two independent assays for expression quantification.

Prior research had developed a number of new compounds making use of a novel drug discovery paradigm which begins with natural products extracted from plants; it then entails selecting synthetic derivatives which demonstrate efficacy in multiple assays testing protection against different factors of the nerve cell damage and death which take place in brain injuries and in age - associated neurodegenerative conditions.

These lipases of different cellular origins share a common function and thus can not be differentiated by use of a catalytic assay such as the ones that are being used to determine lipase activity in serum.

The researchers measured toxicity using a biological assay and found that five common ladybug species each have different levels of toxic defense.

Collected and assayed environmental samples from different processing departments, followed by documenting results and notifying key personnel of any significant findings.

The Quick Start Guide explains the different areas of medical / research laboratory work (Histology, Phlebotomy, Hematology, Microbiology / Bacteriology, Chemistry, Blood Bank, and Molecular) and how the tests (different assays that are used to determine if disease is present, whether it is responding to medication and even, what medications will be most effective) and products (the analyzers, the reagents, and the other tangible items that are used to provide the end result to the attending physician) available fit within those areas.