Sentences with phrase «of different cell lines»

The discovery of NGF has also promoted an intensive search for other specific growth factors, leading to the isolation and characterization of a number of proteins with the ability to enhance the growth of different cell lines.

These findings reveal molecular factors that can be used to evaluate the differentiation potential of different cell lines, which should expedite the progress of iPS cells to clinical use.

But researchers don't have efficient ways to compare the characteristics of different cell lines or to access lines that might be especially useful for a given project.

Not exact matches

The scientists also generated a panel of (reconstructed) ancestral and existing TRIM5 genes (19 total), expressed them in cultured cell lines, and exposed the cells to 16 different retroviruses (lentiviruses and others) to see which TRIM5 versions conferred resistance to which viruses.

Different parts of her body could have come from different ceDifferent parts of her body could have come from different cedifferent cell lines.

That variability among cells containing the same program kicked off a whole new line of experiments that Elowitz says are focused on «what it is that is making different cells do different things.»

As those cells proliferated in laboratory dishes, the bits of human DNA were also copied, creating cell lines, each of which had a different fragment.

DNA from the cell nucleus, which includes both maternal and paternal lines, tells a different story: The two types of elephants are in fact separate species, leading recently to a proposed relisting of the forest elephant as Loxodonta cyclotis.

The enzyme was tested in three different cell lineages: leukemia cells incapable of producing asparagine at normal levels (MOLT4); another line of leukemia cells, in this case capable of producing asparagine at normal levels (REH); and non-malignant cells, used as a control (HUVECs).

Then, they produced the hemagglutanin with different combinations of these mutations in an experimental cell line (testing the mutations in H7N9 viruses themselves could be dangerous).

Research based on such false cell lines would undermine the understanding of different cancers and possible treatments, and clutter the scientific literature with bogus conclusions.

But it contains four pairs of apparently duplicated images, even though they are labeled as showing different cell lines.

Further experiments showed that each iPS cell line had a different pattern of DNA methylation, a molecular coating of the DNA that turns genes on or off in the cell.

In a paper published online yesterday in Stem Cells, the researchers report that they succeeded in generating pluripotent human ES cell lines — i.e., cells that can develop into many different kinds of cells — from one of the 13 late - arrested embCells, the researchers report that they succeeded in generating pluripotent human ES cell lines — i.e., cells that can develop into many different kinds of cells — from one of the 13 late - arrested embcells that can develop into many different kinds of cells — from one of the 13 late - arrested embcells — from one of the 13 late - arrested embryos.

Part of the trouble is the ingredients: Subtle variations in tissue - culture chemicals and Matrigel, or in different stem cell lines and how they are grown first in 2 - D culture, can have a big impact on how the organoids turn out, Novitch says.

Multi-colored metastasis in the peritoneal lining of the abdomen comprised of red and yellow fluorescent cells demonstrating that pancreatic cancer spreads through interactions between different groups of cells.

The team used five types of antibiotics — including one used to treat acne (doxycycline)-- on cell lines of eight different types of tumour and found that four of them eradicated the cancer stem cells in every test.

The researchers then exposed cells from each of these lines to a panel of 31 different drug treatments — including 23 chemotherapy compounds approved by the FDA for breast and ovarian cancers, six targeted cancer drugs, and two common drug combinations.

Although the H3K27M mutation disrupts a different chemical tagging system, the scientists showed that panobinostat slowed the growth of a line of cells that had the mutation.

The resulting «map» of gene - drug interactions allowed the researchers to accurately predict the responses of multiple human cancer cell lines to different chemotherapy agents based on the cell lines» genetic profiles and also revealed new genetic factors that appear to determine the response of breast and ovarian tumor cells to common classes of chemotherapy treatment.

Each branch of the tree is lined with cells sensitive to a different chemical or molecular compound.

«We've seen this in six different types of human cancer cell lines in the lab,» Choudhury said.

In experiments with cancer cell lines, the PIM1 inhibitors killed cells in a MYC - dependent manner, and in two different mouse models — one in which mice were implanted with patient tumors and the other in which a genetic alteration of MYC predisposes the mice to tumor development — the administration of PIM1 inhibitors resulted in significant tumor regression.

By functionally linking the signal transduction of melanopsin to the control circuit of the nuclear factor of activated T cells, we have designed a synthetic signaling cascade enabling light - inducible transgene expression in different cell lines grown in culture or bioreactors or implanted into mice.

There are many hundreds, if not thousands, of different stem cell lines in use around the world.

In the different cell types, the loops begin and end at different specific chromosomal locations, so each cell line's genome appears to have a unique population of loops.

The study used a well - known line of pancreatic cancer cells (AsPC - 1) in the laboratory and assessed how well this grew when treated with either the chemotherapy drug gemcitabine or different levels of commercially available chokeberry extract alone, and when treated with a combination of gemcitabine and chokeberry extract.

Cell lines are therefore indispensable in medical research and a large number of cell lines exist that originate from many different tumor tyCell lines are therefore indispensable in medical research and a large number of cell lines exist that originate from many different tumor tycell lines exist that originate from many different tumor types.

Yet, «these studies using HeLa cells had to rely on information from the «reference» sequence produced by the Human Genome Project, even though there was evidence that the genomes of HeLa cell lines were probably quite different,» said Lars Steinmetz, Ph.D., who led the G3 study.

Recent studies highlighted significant differences between these two types of stem cells, although only a limited number of cell lines of different origins were analyzed.

Different tumor subtypes can include glandular, which include the mammary glands, as well as squamous, which are very rare and involve epithelial cells that line the inside of the breast.

Moreover, cell lines vary in their ability to make different tissues, such as heart muscle or cartilage, so a suite of clinical - grade lines is needed, Braude says.

By May 2005 they had used cloning techniques to create 11 stem cell lines, each one the perfect genetic match of a different patient, another first.

The cell responded to three very different images of the former president: a line drawing of Clinton laughing, a formal painting of him, and a photograph of him mingling with other dignitaries.

They are generating β cells from those cell lines to look for differences that might explain how the different forms of the disease develop.

They tried hundreds of different recipes; eventually they discovered that if they mixed liver precursor cells (derived from iPS cells) with two other types of standard human cell lines known to be important for embryonic liver development, then the cells would spontaneously form a 4 to 5 - millimeter 3D structure called a liver bud.

In principle, tissue banks of parthenogenetic cell lines could include enough different immune protein combinations to treat up to half of the U.S. population — men as well as women — Lanza says.

The researchers tested the drug on mice with H. pylori infections from several different cell lines, and found that the drug was effective against the H. pylori while maintaining populations of healthy gut bacteria.

Initially, the Geneva researchers observed the vascularisation processes of human tumour cells from different cell lines.

In parthenote - derived cell lines, they found a distinctive signature: Most genes were different on the two sets of chromosomes, but genes located close to the chromosome's center were largely identical.

The scientists were thus able to transfer the genetic information from the known fluorescent protein eqFP615 into the DT40 chicken B cell line in order to produce protein variants of the new infrared marker Amrose with different spectral properties.

The generality of this observation was further confirmed by examining the expression of miR - 7 in 2 different cell lines, CN34 and MCF7, and their metastatic variants, CN34BoM, CN34BrM, and MCF7BoM.

The rate of cell proliferation varied only slightly between the different endothelial cell lines (Fig. 3) ⇓.

We capitalized on one of the innate responses of microvascular endothelial cells, i.e., the up - regulation of cell adhesion molecules in response to inflammatory agents, to generate endothelial cell lines from different organs.

The constitutive expression of these endothelial cell adhesion molecules was very similar for most of the lines derived from different organs, i.e., very low expression levels of E-selectin and VCAM - 1 and more pronounced expression of ICAM - 1.

The results, although still preliminary in a quantitative sense, demonstrate that the reactivation of methylation - silenced genes does occur in different cell lines, suggesting it is a general phenomenon.

The selection strategy targeted cell populations expressing the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1, and proved successful in generating microvascular endothelial cell lines from a number of different organs.

However, efforts to examine the in vitro behavior of tumor cells with endothelial cells from different anatomical regions have been prohibited because, in large part, of the limited number of organ - derived endothelial cell lines available for study.

These stem cells live in the lining of the intestine, known as the epithelium, and generate all of the different cell types that make up the epithelium.

The authors studied a standard panel of 60 established human tumor cell lines representing nine different human cancers, as well as several specimens of human primary ovarian cancer.