Sentences with phrase «of electrophoresis»

Other uses of electrophoresis include DNA testing, where electricity is used to separate the parts of a DNA sample by making them move across a gel, which enables them to be compared with other samples and identified.

To control each color of the particles, the company designed them so that the threshold value (electric field) of electrophoresis becomes different for each color.

Not exact matches

«Flow cytometry identified a large population of apoptotic melanoma cells, and single - cell electrophoresis indicated that mensacarcin causes genetic instability, a hallmark of early apoptosis,» Loesgen said.

The appearance of the variant after starch - gel electrophoresis of the hemolysates supports the concept that the lactic dehydrogenase isozymes are determined by the interaction of two subunits which are under separate genetic control.

This allows for the implementation of standard molecular biology tools such as genetic analysis (electrophoresis), amplification (PCR), sample preparation, and purification.

The NLISA platform uses a process call gel electrophoresis to screen synthesized, single strands of DNA «reagents» that change their shape in the presence of a specific biomarker.

In his spare time, he invented gel electrophoresis, one of the workhorse analytical tools of molecular biology.

In combination with gel electrophoresis, that shape change makes it extremely easy to detect whether or not the biomarker of interest is present.

By combining two chemical techniques — narrow capillary electrophoresis and mass spectrometry — Moini figured out how to measure that ratio in just 20 minutes using microscopic samples of silk.

Vijayvargia's team of master's degree students recently designed and built a DNA gel electrophoresis unit with power pack and ultraviolet illuminator that would cost as much as $ 2000 commercially; their version cost about $ 35.

Conventional electrophoresis is performed in slabs of gel.

The ultimate goal is to put not just electrophoresis, but the preparation of DNA and the analysis of the DNA patterns all onto a single device controlled by a built - in computer.

Our new electrophoresis method allows us to increase the number of test samples on a given run by up to ten-fold and to complete the assay in less than half the time of the traditional comet process.»

We hope that this result will accelerate the automation and miniaturization of gel electrophoresis, which in turn can make medical diagnoses and DNA sequencing cheaper, faster, and more reliable,» says Assoc. Prof. Nathaniel Robinson, leader of the research group, on their «work as a logical extension of previous studies on conducting polymer electrodes in electrokinetic systems.»

Although the primary effect of the electric field is cracking cell structures for better availability of nutrition, other effects like shockwave, electrophoresis or influence on the metabolic condition of cells can play a role.

We have compared the transcriptome in blood leukocytes, liver, and brain of humans, chimpanzees, orangutans, and macaques using microarrays, as well as protein expression patterns of humans and chimpanzees using two - dimensional gel electrophoresis.

The polypeptides are then subjected to two rounds of a routine separation technique called gel electrophoresis.

Typhoon supports phosphor imaging, 2D difference gel electrophoresis imaging, and RGB and NIR fluorescence, as well as sensitive and accurate quantitation of proteins, nucleic acids, and other biomolecules.

Researchers from the University of Granada (UGR) have used their own method of «microextraction and capillary electrophoresis» to analyse concentrations of a kind of mycotoxins, patulin, in 19 batches of eight brands of commercial apple juice.

Two - dimensional polyacrylamide gel electrophoresis combined with chromatographic phosphoprotein pre-enrichment and electrospray ionization mass spectrometry - based protein identification was used to reveal changes of phosphoproteins in cells exposed to cyclic stretching.

The success of the PCR amplification was confirmed by electrophoresis and 5 uL of post-PCR reaction product was treated to degrade unused primers and nucleotides using USB ® HT ExoSAP - IT ® High - Throughput PCR Product Cleanup (Affymetrix, http://www.affymetrix.com) according to the manufacturer's instructions.

Primers were chosen from exons separated by large introns, when possible, and PCR reaction quality and specificity were verified by gel electrophoresis and melting - curve dissociation analysis of the amplified product.

Any technology that displaces capillary electrophoresis of STRs absolutely must provide CODIS profiles.

Two - Dimensional Gel Electrophoresis - Based Proteomic Analysis Reveals N - terminal Truncation of the Hsc70 Protein in Cotton Fibers In Vivo.

The 2 alleles differ by a single nucleotide, so restriction digestion of the amplified product followed by gel electrophoresis effectively differentiates the 2 alleles.

Clark's new techniques using capillary electrophoresis and microchip technology may allow for real - time field screening of plutonium and other materials by nuclear proliferation investigators and forensic scientists at the Department of Homeland Security.

Oliver Smithies was a prolific inventor and devised the method of using potato starch as medium for electrophoresis gel.

Microfabricated Channel Array Electrophoresis for Characterization and Screening of Enzymes Using RGS - G Protein Interactions as a Model System.

The improved size resolution of the DNA chip allows students to identify their genotype, something impossible with agarose gel electrophoresis.

Indian scientists have developed a fluorescence - based system that can reveal a whole load of information about proteins separated by gel electrophoresis while they're still embedded in the gel.

Large - scale and effective studies of biological molecules with a different structural basis such as proteins, nucleic acids, carbohydrates, lipids and numerous metabolites (including e.g. fatty acids, amino acids, purines, pyrimidines or steroids) is currently highly attractive because of the availability of modern instruments and tools for both separation and analysis (2 - D chromatographs, capillary electrophoresis systems, microfluidics, mass spectrometers, powerful NMR spectrometers, DNA / RNA arrays and others).

Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].

The authors of this eBook explored direct analysis of bioreactor harvest using capillary sodium - dodecyl sulfate (cSDS) electrophoresis, size - exclusion high - performance liquid chromatography (SE - HPLC), and surface plasmon resonance (SPR) assays.

Equal amounts of protein or conditioned medium were separated by SDS — polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes and probed with the indicated antibodies.

PCR - based assays identify clonality on the basis of over-representation of amplified V — D — J (or incomplete D — J) products following their separation using gel or capillary electrophoresis.

He also pioneered the development of microfabricated capillary electrophoresis devices and microfabricated integrated sample preparation and detection methods for lab - on - a-chip analysis that are being applied to DNA sequencing, diagnostics, forensics, pathogen detection and space exploration.

We offer a comprehensive selection of PCR - based B - and T - cell clonality assays for gel and capillary electrophoresis detection, which also include BIOMED - 2 reagents for clonality and translocations, exclusively offered by Invivoscribe.

Using Surveyor nuclease followed by gel electrophoresis, these mismatches are indicated by the presence of DNA cleavage products.

The FLT3 TKD PCR product is digested with EcoRV restriction enzyme and the presence of the mutation is further assessed using capillary electrophoresis.

The gelatinolytic assays were carried out in two different ways to obtain complementary information: firstly, cells were treated or not with the test compound and samples from these were submitted to gelatinase zymography to detect the effects of the kahweol treatment on the expression of gelatinase activities; secondly, in some experiments, samples from control, untreated HT - 1080 fibrosarcoma cells were submitted to zymography and, after electrophoresis, different concentrations of kahweol were added to the substrate buffer to determine the potential direct effect of kahweol on gelatinase activity.

The projects will provide training in: current molecular biology techniques (including RNA interference, genetic transformation, analyses of gene expression); cell biology techniques (cell culturing, cell transfections, imaging); protein biochemistry (2D electrophoresis, protein functional analyses); and bioinformatics (structure predictions, phylogenetic analyses; molecular interactions).

Protein complexes isolated through affinity purification of a bait protein using an epitope tag and competitive elution are separated through blue native electrophoresis.

Compared to about 800 million bases per week generated at the Sanger Institute in the height of the human genome project using conventional capillary electrophoresis methods, the Illumina production facility currently averages about 300 billion bases (Gigabases = Gb) per week.

The size of the FLT3 ITD and NPM1 PCR products are determined by capillary electrophoresis.

The zeta potentials of the samples were obtained from laser Doppler electrophoresis, converting electrophoretic mobilities to zeta potentials.

Determination of D - saccharic acid -1,4-lactone from brewed kombucha broth by high - performance capillary electrophoresis.

Carrier screening for beta thalassemia and related hemoglobinopathies is recommended by ACOG via complete blood count and hemoglobin electrophoresis for people of African, Southeast Asian, Mediterranean, Middle Eastern, and West Indian descent considering having children.

Carrier screening for hemoglobinopathies such as sickle cell anemia is recommended by the American Congress of Obstetricians and Gynecologists (ACOG) via complete blood count and hemoglobin electrophoresis for people of African, Southeast Asian, Mediterranean, Middle Eastern, and West Indian descent considering having children.

If genetically - high cholesterol is something that is suspected (because of extremely high levels or levels that are non-responsive to care), a lipid electrophoresis can be run to determine if in fact a genetic lipid disorder exists known as hyperlipoproteinemia.

Pyrosequencing of 16S rRNA genes and temporal temperature gradient electrophoresis (TTGE) profiles in the colonic digesta showed that rats fed RS had altered microbiota compositions due to blooms of Bacteroidetes and Actinobacteria.