Sentences with phrase «of electrophoresis gels»

Not exact matches

The appearance of the variant after starch - gel electrophoresis of the hemolysates supports the concept that the lactic dehydrogenase isozymes are determined by the interaction of two subunits which are under separate genetic control.

The NLISA platform uses a process call gel electrophoresis to screen synthesized, single strands of DNA «reagents» that change their shape in the presence of a specific biomarker.

In his spare time, he invented gel electrophoresis, one of the workhorse analytical tools of molecular biology.

In combination with gel electrophoresis, that shape change makes it extremely easy to detect whether or not the biomarker of interest is present.

Vijayvargia's team of master's degree students recently designed and built a DNA gel electrophoresis unit with power pack and ultraviolet illuminator that would cost as much as $ 2000 commercially; their version cost about $ 35.

Conventional electrophoresis is performed in slabs of gel.

We hope that this result will accelerate the automation and miniaturization of gel electrophoresis, which in turn can make medical diagnoses and DNA sequencing cheaper, faster, and more reliable,» says Assoc. Prof. Nathaniel Robinson, leader of the research group, on their «work as a logical extension of previous studies on conducting polymer electrodes in electrokinetic systems.»

We have compared the transcriptome in blood leukocytes, liver, and brain of humans, chimpanzees, orangutans, and macaques using microarrays, as well as protein expression patterns of humans and chimpanzees using two - dimensional gel electrophoresis.

The polypeptides are then subjected to two rounds of a routine separation technique called gel electrophoresis.

Typhoon supports phosphor imaging, 2D difference gel electrophoresis imaging, and RGB and NIR fluorescence, as well as sensitive and accurate quantitation of proteins, nucleic acids, and other biomolecules.

Two - dimensional polyacrylamide gel electrophoresis combined with chromatographic phosphoprotein pre-enrichment and electrospray ionization mass spectrometry - based protein identification was used to reveal changes of phosphoproteins in cells exposed to cyclic stretching.

Primers were chosen from exons separated by large introns, when possible, and PCR reaction quality and specificity were verified by gel electrophoresis and melting - curve dissociation analysis of the amplified product.

Two - Dimensional Gel Electrophoresis - Based Proteomic Analysis Reveals N - terminal Truncation of the Hsc70 Protein in Cotton Fibers In Vivo.

The 2 alleles differ by a single nucleotide, so restriction digestion of the amplified product followed by gel electrophoresis effectively differentiates the 2 alleles.

Oliver Smithies was a prolific inventor and devised the method of using potato starch as medium for electrophoresis gel.

The improved size resolution of the DNA chip allows students to identify their genotype, something impossible with agarose gel electrophoresis.

Indian scientists have developed a fluorescence - based system that can reveal a whole load of information about proteins separated by gel electrophoresis while they're still embedded in the gel.

Ten - microliter aliquots of the nested PCR products were subjected to RFLP analysis by digestion with 2 U of either HinfI or MseI, and digested fragments were resolved by agarose gel electrophoresis in TBE buffer, as described elsewhere [19, 28].

Equal amounts of protein or conditioned medium were separated by SDS — polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes and probed with the indicated antibodies.

PCR - based assays identify clonality on the basis of over-representation of amplified V — D — J (or incomplete D — J) products following their separation using gel or capillary electrophoresis.

We offer a comprehensive selection of PCR - based B - and T - cell clonality assays for gel and capillary electrophoresis detection, which also include BIOMED - 2 reagents for clonality and translocations, exclusively offered by Invivoscribe.

Using Surveyor nuclease followed by gel electrophoresis, these mismatches are indicated by the presence of DNA cleavage products.

The slides then move onto the reaction, gel electrophoresis, autoradiograph production, and some key points about the value of genome sequencing.

Other uses of electrophoresis include DNA testing, where electricity is used to separate the parts of a DNA sample by making them move across a gel, which enables them to be compared with other samples and identified.

The AKC - CHF grant will cover the cost of the renal biopsy / autopsy evaluation (if this is performed), urinalysis, serum chemistry, urine protein gel electrophoresis, and DNA isolation (approximately $ 850 value).

First, she will use the clinical method of gel electrophoresis (commonly used to visualize DNA) to conduct a «hormone race» between cells composed of different ratios of estrogen and testosterone.

• Adept at teaching chromatography and gel electrophoresis • Able to develop and implement chemistry curriculum and lessons aimed at assisting students in learning complex concepts such as organic and chemical chemistry • Strong knowledge of sequence specific recognition principals of DNA binding properties and its implementation in a classroom environment • Complete understanding of preparing interactive course materials including chemistry pilot projects to introduce new chemistry concepts

Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay, DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign