Sentences with phrase «of endogenous genes»

Targeted DNA demethylation and activation of endogenous genes using programmable TALE - TET1 fusion proteins.

Amabile A, Migliara A, Capasso P, Biffi M, Naldini L, Lombardo A. Inheritable silencing of endogenous genes by hit - and - run targeted epigenetic editing.

Kim, Jung - Sik, et al. «Epitope tagging of endogenous genes in diverse human cell lines.»

Not exact matches

Perhaps the most significant distinction between evolution and ID / creationism is evolution's ability to explain poor design features, e.g. male nip - ples, the recurrent laryngeal nerve, the presence / location of endogenous retroviruses, and (one of my personal favorites) the presence of a defunct gene for egg yolk protein in our placental mammal genomes.

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

To do so, the placenta may deploy a mercenary army of endogenous retroviruses — viral genes embedded in the mammal's DNA.

The roadmap outlines future research directions toward the goal of enhancing human radioresistance, including upregulation of endogenous repair and radioprotective mechanisms, possible leeways into gene therapy in order to enhance radioresistance via the translation of exogenous and engineered DNA repair and radioprotective mechanisms, the substitution of organic molecules with fortified isoforms, the coordination of regenerative and ablative technologies, and methods of slowing metabolic activity while preserving cognitive function.

Some of the first genes to start expressing themselves again are former viruses turned into transposable elements known as human endogenous retroviruses, particularly one called HERVK, Wysocka and colleagues found.

The researchers also found that many endogenous genes had additional repeating sections of A and T nucleotides that mark them as «self» and not foreign.

The more powerful one occurred within a stretch of DNA, or locus, that contains the HCP5 gene, which codes for a human endogenous retrovirus — a genetic fossil of a virus that wove itself into human chromosomes long ago but no longer produces infectious progeny.

Because of these limitations, the AAV approach is best suited for tissue culture based approaches that require modification of only a single allele of a gene, such as the introduction of heterozygous endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele in cultured cells.

Some examples from their lab include using AAV to introduce epitope tags into the endogenous alleles of the p53 and PTEN tumor suppressor genes in human cells (Kim et al 2008).

They have also used AAV gene editing to introduce naturally occurring cancer - causing mutations into the endogenous allele of the STAG2 tumor suppressor in human cells -LRB-

They have also used AAV gene editing to introduce naturally occurring cancer - causing mutations into the endogenous allele of the STAG2 tumor suppressor in human cells (Kim et al, 2016).

Kin Ritchie, Ralph Brinster, and Ursula Storb show that an IgL transgene inhibits rearrangement of endogenous IgL genes, providing the first clue for the mechanism of allelic exclusion of Ig genes.

Workflow for targeted knockout of CD81, an endogenous gene in hiPS cells, using the Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System.

After concluding from our proof - of - concept experiment that a successful gesicle - based knockout of a virally integrated gene can be achieved, we chose to target CD81, an endogenous membrane glycoprotein that forms complexes with integrins and plays a critical role in the infection process that leads to hepatitis C (Figure 3).

In tobacco, we measured a reduction in the endogenous POR genes (NtPOR), and induction of the AtRCCR and AtChlase transgenes in response to TNT.

Multiple mouse models of SMA have been produced, generally by knocking out the endogenous mouse SMN1 gene and replacing it with one or more copies of the human SMN2 gene that produces much less full - length SMN protein than the SMN1 gene.

Quantitative RT - PCR analysis of the de-greening circuit genes (Figure 4E) corroborates the weak response observed in the transgenic plants, with endogenous NtPOR mRNA levels decreasing to approximately one - third of the control levels, and AtRCCR increasing three-fold.

In the new study, a team led by Harvard University's George Church — one of the pioneers of the CRISPR technique — used gene editing to remove all the copies of porcine endogenous retroviruses (or PERVs) from their pig cells» DNA.

This conclusion is reinforced by the findings of Buller et al. (J: 8497), who showed that the Rmcfr allele contains an endogenous MCF gp70 env gene and that the Rmcfs allele, at least in some strains (C57BL / 6, CBA / J, and A / WySn), contains a xenotropic gp70 env gene.

To accomplish this, we used homologous recombination to ablate nonmuscle myosin heavy chain (NMHC) II - B by inserting cDNA encoding green fluorescent protein (GFP)- NMHC II - A into the first coding exon of the Myh10 gene, thereby placing GFP - NMHC II - A under control of the endogenous II - B promoter.

Bach1 has an endogenous ligand, heme, that inhibits Bach1 binding to ARE, thus allowing Nrf2 - mediated gene expression including that of heme - oxygenase - 1 (HMOX1), a well described target of Bach1 repression.

CRISPR has allowed scientists to tag and light up endogenous genes of interest to better understand normal protein function.

The use of endogenous HMOX1 protein expression as a readout allowed the identification of compounds that specifically derepress Bach1 and induce transcription of an Nrf2 - responsive gene.

In mouse models in which the endogenous Smn1 gene has been knocked out and human versions of SMN2 have been swapped in, the Isis therapy — a so - called «antisense oligonucleotide» — delivered to the mouse central nervous system (CNS) increased the expression of full - length SMN protein in motor neurons, improved muscle strength in behavioral tests and extended the rodents» median lifespan from 16 days to 26 days3.

Cas9 / gRNA can be easily engineered against almost all endogenous genes within a 3 - day period of time, and goats carrying multiplex mutations can be generated within 5 months (gestation length 150 — 155 days).

Recently, prospects for xenotransplantation brightened when Harvard geneticist George Church demonstrated the removal of dozens of endogenous retroviruses from the pig genome, in a tour de force of the CRISPR / Cas9 gene editing technique.

Introduction of DNA double - strand breaks by site - specific endonucleases called meganucleases is being successfully used to target endogenous genes in human cells.

In each of the BAC transgenic vectors, endogenous protein coding sequences have been replaced by sequences encoding the EGFP reporter gene.

Thus these results measure the relative rates of transcription for each gene; they are not a direct measure of mRNA accumulation or of protein abundance for the endogenous gene products.

His pioneering mouse embryonic stem (ES) cell work in the 1980s, demonstrating germ - line transmission and the great potential of ES cells to generate mice carrying mutations in endogenous genes, established milestones in a field that saw the award of the 2007 Nobel Prize in Physiology or Medicine to Mario Capecchi, Martin Evans, and Oliver Smithies.

The aim of WICT is the removal from the organismal environment of accumulated cellular and intracellular damage present in the patient's endogenous cells, including telomere depletion, nuclear DNA damage and mutations, mitochondrial DNA damage and mutations, replicative senescence, functionally - deleterious age - related changes in gene expression and accumulated cellular and intracellular aggregates.

That means I need to find ways to increase BDNF more than in someone with the normal gene in order to preserve my cognitive function, memory, and emotional regulation.8 Mu, J.S., et al. «Deprivation of Endogenous Brain - Derived Neurotrophic Factor Results in Impairment of Spatial Learning and Memory in Adult... continue 9Cirulli, F., et al. «Intrahippocampal Administration of BDNF in Adult Rats Affects Short - term Behavioral Plasticity in the Morris Water Maze and... continue