Sentences with phrase «of endothelial cells with»

Not exact matches

They first devised a nanoscale plastic scaffold and then seeded it with common muscle precursor cells called myoblasts, along with endothelial cells of the sort found on the inside of blood vessels.

«The effect of the precursors that boost NAD is to counteract the decline that occurs with normal aging, to reactivate SIRT1, and to restore function in endothelial cells to give rise to more blood vessels.»

In a collaborative effort between the Gladstone laboratories of Benoit Bruneau, PhD, Katherine Pollard, PhD, and Dr. Srivastava, the scientists used stem cell technology to make large amounts of endothelial cells from patients with CAVD, comparing them to healthy cells and mapping their genetic and epigenetic changes as they developed into valve cells.

In the chamber, tubes about the thickness of a human hair were lined with endothelial cells as in natural blood vessels.

They found that, while the grafts of undifferentiated iPS cells harbored large numbers of T cells of only a few specificities — indicating a robust immune response — those found in grafts of the two types of endothelial cells were more diverse, suggesting a more limited response associated with a phenomenon known as self - tolerance.

When zebrafish with the green fluorescing endothelial gene matured, the researchers observed green FGPs on the surface of the fish's brains — confirming that these cells arose from endothelial tissue.

Professor Takao Hamakubo's group at the Department of Quantitative Biology and Medicine, Research Center for Advanced Science and Technology (RCAST), have shown that PTX3 forms strong bonds with histones and partially unfolds, leading to a disordered coaggregation of histone and PTX3 and protecting human endothelial cells from damage.

In contrast, the elongated nanorods have a larger surface area that is in contact with the surface of the endothelial cells.

Researchers from Massachusetts Eye and Ear have, for the first time, identified rapidly proliferating cells (known as «neural crest - derived progenitor cells») in the corneal endothelium of specimens from normal corneas and from corneas with Fuchs» Endothelial Corneal Dystrophy (FECD), a condition in which the cells responsible for keeping the cornea clear die prematurely — often leading to blindness.

The epithelium's maturation into a villus intestinal epithelium with long finger - like extensions was helped along by co-culturing human intestinal microvascular endothelial cells on the opposite side of the shared matrix - coated porous membrane in the «vascular» channel where they assembled a surrogate blood vessel with a hollow lumen through which feeding medium was flowed.

To verify that the process worked, the endothelial cells with the inversion - corrected genes were transplanted into F8 deficient mice (mice with hemophilia A) and the mice started producing the F8 clotting factor on their own, which essentially cured them of hemophilia A.

«ECPR and a factor in the blood called protein C act as a «brake» on blood coagulation and endothelial cell inflammation and also enhance the viability and integrity of blood vessels, but when the malaria parasites use PfEMP1 to bind EPCR, they may interfere with the normal function of EPCR, and thus the binding can be the catalyst for the violent reaction,» he explains.

To study this barrier and determine why a lack of blood flow causes it to leak, the researchers built a blood - vessel - on - a-chip model consisting of a channel lined with a layer of human endothelial cells surrounded by extracellular matrix within a microfluidic device, which allowed them to easily simulate and control the flow of blood through a vessel and evaluate the cells» responses.

This experiment uses quantitative PCR to detect the expression level of POSTN in CD34 + / CD31 − pulmonary fibroblasts, CD31 + endothelial cells, and CD45 + immune cells isolated from lungs of mice with macrometastases, which is a replication of the experiment reported in Figure 2H.

B, CSCs isolated from 231BrM infected with lentivirus of miR7 or KLF4 expression plasmids were seeded on top of mouse brain endothelial cells or Matrigel.

Matrigel - containing plates were allowed to incubate at 37 °C for 30 min and then seeded with endothelial cells at a density of 4 × 104 cells / well in 10 % DMEM.

Phase - contrast images of organ - derived endothelial cells (37 °C) on Matrigel surface (A) and after 4 h of incubation with DiI - Ac - LDL (B).

One of the primary objectives of this study was to establish a protocol to facilitate the identification and selection of microvascular endothelial cells from primary organ cultures with a high degree of precision.

We have observed on several occasions that, for the majority of endothelial cell lines, the transfer to 37 °C conditions for 72 h correlated with a 50 % reduction in cell proliferation, a value that continued to decrease with time.

As expected, all of the endothelial cell lines showed diminished constitutive expression of E-selectin (data not shown) and VCAM - 1 (Fig. 4C) ⇓ in comparison with surface levels of ICAM - 1 (Fig. 4B) ⇓.

A, CSCs isolated from 231BrM and MB231 with the ectopic expression of miR7 and miR7 - LNA were seeded on top of mouse brain endothelial cells or Matrigel.

To determine inducible endothelial cell adhesion molecule expression (E-selectin, VCAM - 1, and ICAM - 1), some of the chambers were given medium containing 10 ng / ml TNF - α for 4 — 6 h (these time points were selected to correlate with the known kinetics of endothelial cell adhesion molecule expression; Refs.

Endothelial cells derived from the brain demonstrated a complete absence of uptake after 4 h of incubation (at 33 °C or 37 °C) with this fluorescent reagent (data not shown).

However, efforts to examine the in vitro behavior of tumor cells with endothelial cells from different anatomical regions have been prohibited because, in large part, of the limited number of organ - derived endothelial cell lines available for study.

In B, endothelial cells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldecells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldecells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldeCells were washed with label - free medium and fixed in 4 % paraformaldehyde.

The onset of tube structure formation could be visualized as early as 4 h after plating for most endothelial cell lines, with extensive networks becoming visible within 12 h after plating.

Pilot studies using a variety of strategies suggested that this goal could be best achieved by stimulating primary organ cell cultures with TNF - α (10 ng / ml) and targeting cells expressing the endothelial cell adhesion molecules E-selectin and VCAM - 1 through a FACS - driven strategy.

The present demonstration of T cell - mediated apoptosis of allogeneic corneal cells from CD4 KO mice is consistent with previous findings, which noted the presence of apoptotic keratocytes and corneal endothelial cells in rejected corneal allografts in humans and rats respectively (5, 32).

«We're very interested in following up by finding more direct proof that if you block the recruitment of immune cells to tumors, you can reverse this phenomenon in these animals with endothelial cell insulin resistance,» Rask - Madsen says.

In keeping with this, recent studies showed that flavanols, a subclass of flavonoids that is richly represented in natural cocoa beans, increase NO production by cultured human vascular endothelial cells (16) and improve endothelium - dependent vasorelaxation (NO - dependent) in finger (2) and brachial (3) arteries of healthy humans.

The scientists began with a line of mice in which the gene for the insulin receptor has been knocked out in endothelial cells only.

MicroRNA - 24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin - a3 and regulating inflammatory responses.

In the case of rabbit corneal endothelial cells, which are known to divide with regularity, AP - BrdU staining was always seen following a mechanical wound, as shown in Figure 3.

If we understand the special features of insulin resistance in endothelial cells, we may be able to find more effective treatments against artery damage and cardiovascular disease in people with diabetes.»

Briefly, single - cell suspensions of C57BL / 6 KO and wild - type lymphocytes, which had undergone a 96 hr in vitro boost with γ - irradiated (3,000 rad) BALB / c stimulator lymphocytes, were added to 96 - well microtiter plates (Corning Inc., Corning, NY) along with 2 × 104 51Cr - labeled BALB / c corneal epithelial or endothelial cells in a total volume of 200 μl / well.

With potential therapeutic implications, Stainier and a colleague also recently discovered that endothelial cells, which line the blood vessels, play a critical role in inducing the development, or proper differentiation, of red blood cells, the transporters of oxygen.

Extended life of human corneal endothelial cells transfected with the SV40 large T antigen.

This gave a mixed population of cells with 20 % expressing CD34, a hematopoietic stem cell marker, with some co-expression of endothelial cell markers (CD31, Flk1, and VE - cadherin).

Researchers from the laboratories of Hiroshi Y. Yoshikawa and Hideki Taniguchi had previously demonstrated the in vitro formation of a 3D transplantable liver «organ bud» from human induced pluripotent stem cells (iPSCs) co-cultured with mesenchymal and endothelial progenitors, and allows for the growth of a small vascularized and functional organ [1 - 3].

(3,18; cf. 19,20) Even in visceral fat, it has recently emerged that the obesity - driven rise in inflammation and insulin resistance is associated with an abnormal accumulation of senescent cells, albeit senescent endothelial cells rather than adipocytes.

A microscopic image of endothelial cells treated with drug - loaded nanoparticles.

Unlike peripheral capillaries, which allow the relatively free exchange of substances with the surrounding tissue, the capillaries in the brain are tightly packed with endothelial cells.

A team at the Harvard John A. Paulson School for Engineering and Applied Sciences (SEAS) and the Wyss Institute for Biologically Inspired Engineering at Harvard University has invented a method for 3D bioprinting thick vascularized tissue constructs composed of human stem cells, extracellular matrix, and circulatory channels lined with endothelial blood vessel cells.

Project: A Study of the Dynamics of Schlemm's Canal Endothelial Cells using a Three - dimensional Cell Culture Device with Real - time Imaging.

In fact, several of the best characterized anti-angiogenic compounds were initially detected and selected for their capability to interfere with endothelial cell growth.

We also found that the EphB4 receptor expressed on the surface of breast cancer cells can promote tumor xenograft growth by enhancing blood vessel formation through interactions with its preferred ligand, ephrin - B2, present in tumor endothelial cells.

Survival curves of proliferative (squares) and non-proliferative (circles) HUVEC endothelial cells treated with kahweol.

This is the case of the extremely selective inhibitor of endothelial cell proliferation TNP - 470, a synthetic analog of fumagillin with enhanced anti-angiogenic properties [22], [23].

Susan Amara, USA - «Regulation of transporter function and trafficking by amphetamines, Structure - function relationships in excitatory amino acid transporters (EAATs), Modulation of dopamine transporters (DAT) by GPCRs, Genetics and functional analyses of human trace amine receptors» Tom I. Bonner, USA (Past Core Member)- Genomics, G protein coupled receptors Michel Bouvier, Canada - Molecular Pharmacology of G protein - Coupled Receptors; Molecular mechanisms controlling the selectivity and efficacy of GPCR signalling Thomas Burris, USA - Nuclear Receptor Pharmacology and Drug Discovery William A. Catterall, USA (Past Core Member)- The Molecular Basis of Electrical Excitability Steven Charlton, UK - Molecular Pharmacology and Drug Discovery Moses Chao, USA - Mechanisms of Neurotophin Receptor Signaling Mark Coles, UK - Cellular differentiation, human embryonic stem cells, stromal cells, haematopoietic stem cells, organogenesis, lymphoid microenvironments, develomental immunology Steven L. Colletti, USA Graham L Collingridge, UK Philippe Delerive, France - Metabolic Research (diabetes, obesity, non-alcoholic fatty liver, cardio - vascular diseases, nuclear hormone receptor, GPCRs, kinases) Sir Colin T. Dollery, UK (Founder and Past Core Member) Richard M. Eglen, UK Stephen M. Foord, UK David Gloriam, Denmark - GPCRs, databases, computational drug design, orphan recetpors Gillian Gray, UK Debbie Hay, New Zealand - G protein - coupled receptors, peptide receptors, CGRP, Amylin, Adrenomedullin, Migraine, Diabetes / obesity Allyn C. Howlett, USA Franz Hofmann, Germany - Voltage dependent calcium channels and the positive inotropic effect of beta adrenergic stimulation; cardiovascular function of cGMP protein kinase Yu Huang, Hong Kong - Endothelial and Metabolic Dysfunction, and Novel Biomarkers in Diabetes, Hypertension, Dyslipidemia and Estrogen Deficiency, Endothelium - derived Contracting Factors in the Regulation of Vascular Tone, Adipose Tissue Regulation of Vascular Function in Obesity, Diabetes and Hypertension, Pharmacological Characterization of New Anti-diabetic and Anti-hypertensive Drugs, Hypotensive and antioxidant Actions of Biologically Active Components of Traditional Chinese Herbs and Natural Plants including Polypehnols and Ginsenosides Adriaan P. IJzerman, The Netherlands - G protein - coupled receptors; allosteric modulation; binding kinetics Michael F Jarvis, USA - Purines and Purinergic Receptors and Voltage-gated ion channel (sodium and calcium) pharmacology Pain mechanisms Research Reproducibility Bong - Kiun Kaang, Korea - G protein - coupled receptors; Glutamate receptors; Neuropsychiatric disorders Eamonn Kelly, Prof, UK - Molecular Pharmacology of G protein - coupled receptors, in particular opioid receptors, regulation of GPCRs by kinasis and arrestins Terry Kenakin, USA - Drug receptor pharmacodynamics, receptor theory Janos Kiss, Hungary - Neurodegenerative disorders, Alzheimer's disease Stefan Knapp, Germany - Rational design of highly selective inhibitors (so call chemical probes) targeting protein kinases as well as protein interaction inhibitors of the bromodomain family Andrew Knight, UK Chris Langmead, Australia - Drug discovery, GPCRs, neuroscience and analytical pharmacology Vincent Laudet, France (Past Core Member)- Evolution of the Nuclear Receptor / Ligand couple Margaret R. MacLean, UK - Serotonin, endothelin, estrogen, microRNAs and pulmonary hyperten Neil Marrion, UK - Calcium - activated potassium channels, neuronal excitability Fiona Marshall, UK - GPCR molecular pharmacology, structure and drug discovery Alistair Mathie, UK - Ion channel structure, function and regulation, pain and the nervous system Ian McGrath, UK - Adrenoceptors; autonomic transmission; vascular pharmacology Graeme Milligan, UK - Structure, function and regulation of G protein - coupled receptors Richard Neubig, USA (Past Core Member)- G protein signaling; academic drug discovery Stefan Offermanns, Germany - G protein - coupled receptors, vascular / metabolic signaling Richard Olsen, USA - Structure and function of GABA - A receptors; mode of action of GABAergic drugs including general anesthetics and ethanol Jean - Philippe Pin, France (Past Core Member)- GPCR - mGLuR - GABAB - structure function relationship - pharmacology - biophysics Helgi Schiöth, Sweden David Searls, USA - Bioinformatics Graeme Semple, USA - GPCR Medicinal Chemistry Patrick M. Sexton, Australia - G protein - coupled receptors Roland Staal, USA - Microglia and neuroinflammation in neuropathic pain and neurological disorders Bart Staels, France - Nuclear receptor signaling in metabolic and cardiovascular diseases Katerina Tiligada, Greece - Immunopharmacology, histamine, histamine receptors, hypersensitivity, drug allergy, inflammation Georg Terstappen, Germany - Drug discovery for neurodegenerative diseases with a focus on AD Mary Vore, USA - Activity and regulation of expression and function of the ATP - binding cassette (ABC) transporters

Figure 4 shows the mean survival curves obtained with the MTT assay in human umbilical vein endothelial cells (HUVEC), under conditions of normal and low proliferation rates (20 % and 2 % of FBS).