Sentences with phrase «of endothelial tissue»

Masanori Aikawa, an associate professor of medicine at Harvard Medical School, describes the new technology as «a monumental contribution» that should help researchers develop new treatments and learn more about diseases of endothelial tissue such as atherosclerosis and diabetic retinopathy, which can cause blindness.

The dissection of the endothelial tissues is diagrammed in Figure 1.

Not exact matches

This happens because the loss of blood flow in a vessel causes the local oxygen level to drop, which stimulates local production of vascular endothelial growth factor, or VEGF, a protein which in most tissues causes the growth of new blood vessels to repair damage.

Epithelial cells form tissue layers that cover our skin and the inner surfaces of most of our internal organs, while endothelial cells line the adjacent blood - transporting vessels and capillaries that support their functions.

Current thinking regarding type III hypersensitivity is that immune cells within tissues sense the presence of these immune complexes (ICs) through specific receptor molecules and release inflammatory factors called cytokines that activate the endothelial cells lining adjacent blood vessels to promote the recruitment of neutrophils.

Using a powerful imaging technique that allowed the scientists to track the presence and movement of parasites in living tissues, the researchers found that Toxoplasma infects the brain's endothelial cells, which line blood vessels, reproduces inside of them, and then moves on to invade the central nervous system.

When zebrafish with the green fluorescing endothelial gene matured, the researchers observed green FGPs on the surface of the fish's brains — confirming that these cells arose from endothelial tissue.

Researchers from the UNC School of Medicine have discovered that cells called fibroblasts, which normally give rise to scar tissue after a heart attack, can be turned into endothelial cells, which generate blood vessels to supply oxygen and nutrients to the injured regions of the heart, thus greatly reducing the damage done following heart attack.

The transplanted stem cells had differentiated into endothelial cells — which form the inner lining of a blood vessel, providing a barrier between blood and spinal cord tissue — and attached to capillaries in the spinal cord.

Lymphocytes constantly circulate through blood vessels within lymph nodes and other lymphoid organs, but only some actually enter lymphoid tissue by crossing the walls of the vessels, known as high endothelial venules (HEVs).

Key elements of the immune system, they also have the ability to turn into several cell types after having passed the endothelial barrier, in order to fulfil different functions depending on the tissue.

Endothelial cells form the innermost layer of the blood vessels - the endothelium - and have the function of containing blood inside the vessels while allowing the passage of nutrients to tissues.

To overcome these limitations, we used two - color flow cytometry to identify and select microvascular endothelial cells from primary cultures obtained from different organs of mice whose tissues harbor a temperature - sensitive SV40 large T antigen (H - 2Kb - tsA58 mice; ImmortoMice).

One of the major impediments to obtaining a large number of endothelial cells from different tissues has been the inability to purify and propagate these cells in culture.

Tissue - specific microvascular endothelial cell lines from H - 2K (b)- tsA58 mice for studies of angiogenesis and metastasis

Three types of structural variations were visible in the tissue - specific endothelial cell lines (Fig. 2) ⇓.

This happens because the loss of blood flow in a blood vessel causes the local oxygen level to drop, which stimulates local production of vascular endothelial growth factor, or VEGF, a protein that in most tissues causes the growth of new blood vessels to repair damage.

In B, endothelial cells from different tissues were seeded at a density of 1 × 105 cells / chamber in two - chamber slides and incubated with 10 mg / ml DiI - Ac - LDL in 10 % DMEM for 4 h. Cells were washed with label - free medium and fixed in 4 % paraformaldehyde.

Figure 2 shows sets of results for four areas of human corneal endothelial tissue; central endothelium (defined by endothelial cells within a 4 mm trephined boundary), central - intermediate endothelium (defined by endothelial cells within an 8 mm trephined boundary), intermediate - peripheral endothelium (defined by endothelial cells between the 4 mm trephine edge and 12 mm from the center of the corneal endothelium), and the peripheral endothelium (defined by the 8 mm trephined edge and 12 mm from the center of the corneal endothelium).

The fixed tissues were covered on the endothelial side with a 1:1000 dilution of anti-BrdU primary antibody.

To obtain a viable donor of a Descemet membrane endothelium disc, we modified the Descemet membrane stripping technique and monitored the percentage of endothelial damage to the donor tissue preparation.

For each assay, 200 μl of sample (taken from the Optisol solution bathing the human and rabbit corneal endothelial tissues) was added to microplates coated with TGF - β receptor.

Stimulation of Transforming Growth Factor - ß1 - Induced Endothelial - To - Mesenchymal Transition and Tissue Fibrosis by Endothelin - 1 (ET - 1): A Novel Profibrotic Effect of ET - 1.

We will combine methods from the fields of immunology, developmental biology and angiogenesis to understand in vivo the development and lineage - specific function (s) of resident macrophages, thereby opening new venues of research into the interaction between macrophages and endothelial cells in response to tissue damage.

Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue.

The postnatal adipose tissue contains a heterogeneous population of cells which includes adult stem cells (i.e., MSCs), endothelial progenitor cells, leukocytes, endothelial cells, and vascular smooth muscle cells.

Co-culture of bone marrow fibroblasts and endothelial cells on modified polycaprolactone substrates for enhanced potentials in bone tissue engineering.

Unlike peripheral capillaries, which allow the relatively free exchange of substances with the surrounding tissue, the capillaries in the brain are tightly packed with endothelial cells.

Expression and function of the homeostatic molecule Del - 1 in endothelial cells and the periodontal tissue.

A team at the Harvard John A. Paulson School for Engineering and Applied Sciences (SEAS) and the Wyss Institute for Biologically Inspired Engineering at Harvard University has invented a method for 3D bioprinting thick vascularized tissue constructs composed of human stem cells, extracellular matrix, and circulatory channels lined with endothelial blood vessel cells.

â $ œWe have solved a major obstacle of using anti-miRNA as a therapeutic by being able to do a targeted delivery to only inflamed endothelial cells while all other tissues examined, including liver, lung, kidney, blood cells, spleen, etc showed no detectable side - effects, â $ Jo says.

Susan Amara, USA - «Regulation of transporter function and trafficking by amphetamines, Structure - function relationships in excitatory amino acid transporters (EAATs), Modulation of dopamine transporters (DAT) by GPCRs, Genetics and functional analyses of human trace amine receptors» Tom I. Bonner, USA (Past Core Member)- Genomics, G protein coupled receptors Michel Bouvier, Canada - Molecular Pharmacology of G protein - Coupled Receptors; Molecular mechanisms controlling the selectivity and efficacy of GPCR signalling Thomas Burris, USA - Nuclear Receptor Pharmacology and Drug Discovery William A. Catterall, USA (Past Core Member)- The Molecular Basis of Electrical Excitability Steven Charlton, UK - Molecular Pharmacology and Drug Discovery Moses Chao, USA - Mechanisms of Neurotophin Receptor Signaling Mark Coles, UK - Cellular differentiation, human embryonic stem cells, stromal cells, haematopoietic stem cells, organogenesis, lymphoid microenvironments, develomental immunology Steven L. Colletti, USA Graham L Collingridge, UK Philippe Delerive, France - Metabolic Research (diabetes, obesity, non-alcoholic fatty liver, cardio - vascular diseases, nuclear hormone receptor, GPCRs, kinases) Sir Colin T. Dollery, UK (Founder and Past Core Member) Richard M. Eglen, UK Stephen M. Foord, UK David Gloriam, Denmark - GPCRs, databases, computational drug design, orphan recetpors Gillian Gray, UK Debbie Hay, New Zealand - G protein - coupled receptors, peptide receptors, CGRP, Amylin, Adrenomedullin, Migraine, Diabetes / obesity Allyn C. Howlett, USA Franz Hofmann, Germany - Voltage dependent calcium channels and the positive inotropic effect of beta adrenergic stimulation; cardiovascular function of cGMP protein kinase Yu Huang, Hong Kong - Endothelial and Metabolic Dysfunction, and Novel Biomarkers in Diabetes, Hypertension, Dyslipidemia and Estrogen Deficiency, Endothelium - derived Contracting Factors in the Regulation of Vascular Tone, Adipose Tissue Regulation of Vascular Function in Obesity, Diabetes and Hypertension, Pharmacological Characterization of New Anti-diabetic and Anti-hypertensive Drugs, Hypotensive and antioxidant Actions of Biologically Active Components of Traditional Chinese Herbs and Natural Plants including Polypehnols and Ginsenosides Adriaan P. IJzerman, The Netherlands - G protein - coupled receptors; allosteric modulation; binding kinetics Michael F Jarvis, USA - Purines and Purinergic Receptors and Voltage-gated ion channel (sodium and calcium) pharmacology Pain mechanisms Research Reproducibility Bong - Kiun Kaang, Korea - G protein - coupled receptors; Glutamate receptors; Neuropsychiatric disorders Eamonn Kelly, Prof, UK - Molecular Pharmacology of G protein - coupled receptors, in particular opioid receptors, regulation of GPCRs by kinasis and arrestins Terry Kenakin, USA - Drug receptor pharmacodynamics, receptor theory Janos Kiss, Hungary - Neurodegenerative disorders, Alzheimer's disease Stefan Knapp, Germany - Rational design of highly selective inhibitors (so call chemical probes) targeting protein kinases as well as protein interaction inhibitors of the bromodomain family Andrew Knight, UK Chris Langmead, Australia - Drug discovery, GPCRs, neuroscience and analytical pharmacology Vincent Laudet, France (Past Core Member)- Evolution of the Nuclear Receptor / Ligand couple Margaret R. MacLean, UK - Serotonin, endothelin, estrogen, microRNAs and pulmonary hyperten Neil Marrion, UK - Calcium - activated potassium channels, neuronal excitability Fiona Marshall, UK - GPCR molecular pharmacology, structure and drug discovery Alistair Mathie, UK - Ion channel structure, function and regulation, pain and the nervous system Ian McGrath, UK - Adrenoceptors; autonomic transmission; vascular pharmacology Graeme Milligan, UK - Structure, function and regulation of G protein - coupled receptors Richard Neubig, USA (Past Core Member)- G protein signaling; academic drug discovery Stefan Offermanns, Germany - G protein - coupled receptors, vascular / metabolic signaling Richard Olsen, USA - Structure and function of GABA - A receptors; mode of action of GABAergic drugs including general anesthetics and ethanol Jean - Philippe Pin, France (Past Core Member)- GPCR - mGLuR - GABAB - structure function relationship - pharmacology - biophysics Helgi Schiöth, Sweden David Searls, USA - Bioinformatics Graeme Semple, USA - GPCR Medicinal Chemistry Patrick M. Sexton, Australia - G protein - coupled receptors Roland Staal, USA - Microglia and neuroinflammation in neuropathic pain and neurological disorders Bart Staels, France - Nuclear receptor signaling in metabolic and cardiovascular diseases Katerina Tiligada, Greece - Immunopharmacology, histamine, histamine receptors, hypersensitivity, drug allergy, inflammation Georg Terstappen, Germany - Drug discovery for neurodegenerative diseases with a focus on AD Mary Vore, USA - Activity and regulation of expression and function of the ATP - binding cassette (ABC) transporters

For induction of differentiation to mature endothelial cells, EPCs were plated at a high cell density (8 × 104 cells / cm2) on tissue culture treated flasks.

However, in this investigation no isoform of p73 was found in endothelial tissues.

Since stem cells have been found to be isolated at the peripheral / limbal region of the epithelium [7 - 9], this study also investigates cell division potential in central and peripheral endothelial tissue.

The endothelial tissue from these samples was dissected and samples were analyzed for mRNA transcription of p53, transactivating p63 (TAp63), delta N p63 (ΔNp63), transactivating p73 (TAp73), and delta N p73 (ΔNp73) via the reverse transcriptase - polymerase chain reaction (RT - PCR).

Samples of central and peripheral human corneal endothelial tissue were obtained as described above.

At the same time, the protein Vascular Endothelial Growth Factor (VEGF) stimulates blood supply for the damaged tissue, Fibroblast Growth Factor - 2 (FGF - 2) stimulates the damaged cells to grow and reproduce themselves, Transforming Growth Factor - beta (TGF - beta) stimulates cartilage to grow, and Stem Cell Factor (SCF) stimulates your native inactive stem cells to become activated and assist in the repair of the damaged tissue.

We will continue to explore Chrysalin's therapeutic value in tissues and diseases exhibiting endothelial dysfunction as well as the science behind and potential of Chrysalin.