As the level
of gene activity knockdown associated with transgenic RNAi depends on the level of expression of the hairpin constructs, we generated a number of derivatives of our initial vector, called the «VALIUM» series, to improve the efficiency of the method.
These studies will investigate the consequences
of ApoF
knockdown on cholesterol disposition in an animal model, quantify ApoF levels in human subjects and identify lipoprotein properties that cause ApoF to bind and subsequently inhibit cholesteryl ester transfer protein
activity, and investigate the molecular mechanisms controlling ApoF
gene expression.