Sentences with phrase «of gene delivery»

Physical Methods for Gene Transfer: Improving the Kinetics of Gene Delivery into Cells.

That's because scientists have trouble carefully controlling the results of gene delivery: They can't deliver a large effect without also delivering a large risk [source: Friedmann].

«To date, this type of system has only been used in humans with viral methods of gene delivery, of which the safety profiles are still heavily in debate,» says Betty Tyler, associate professor of neurosurgery at Johns Hopkins.

In addition to revealing that biodegradable polymeric nanoparticles represent a promising mode of gene delivery for glioma, the findings show that nonviral DNA delivery of HSVtk combined with administration of ganciclovir has potent antitumor effects.

Not exact matches

Gene therapy involves the delivery, using a modified virus, of a healthy copy of a gene to make up for one that's deficient in a way that causes diseGene therapy involves the delivery, using a modified virus, of a healthy copy of a gene to make up for one that's deficient in a way that causes disegene to make up for one that's deficient in a way that causes disease.

HMOs vary between pre-term and full term birth, vaginal deliveries and planned Cesarean births (reviewed in [20]-RRB-, and even between mothers with different types of «secretor» genes [21].

These fatty molecules have shown promise as delivery vehicles for RNA interference, a process that allows disease - causing genes to be turned off with small strands of RNA.

This makes possible a wide range of biomedical applications such as tissue engineering as well as drug and gene delivery.

The team now plans to improve their gene - delivery technique in an attempt to reach an even greater proportion of hair cells.

To use viruses as delivery vehicles for gene therapy, researchers take all the harmful and replicative genes out of the virus and put in the therapeutic genes they want to deliver.

This approach may make gene therapy a viable method for delivery of polypeptides that require rapid and regulated delivery.

«Gene therapy restores hearing in deaf mice, down to a whisper: Improved delivery vector better penetrates the inner ear, also restores balance in a mouse model of Usher syndrome.»

When oxygen is scarce, however, HIF - 1α starts to accumulate and turns on the expression of a host of genes that assist in promoting and increasing oxygen delivery to hypoxic regions.

A plasmid, a simple kind of DNA - delivery vehicle, will move a gene for antibiotic resistance into the bacterial cells, jump - starting the Crispr - Cas9 system.

Muscle biologists Qi Long Lu and Terence Partridge at the Medical Research Council Clinical Sciences Centre in London, U.K., and their colleagues decided to combined the antisense strategy with a chemical often used in gene therapy because it is known to improve delivery of DNA into cells.

Initial tests on mice showed the hybrid virus was very efficient: the gene it carried was active in 24 per cent of airway cells after two months, a far better proportion than achieved by other delivery methods (New Scientist, 10 March 2001, p 19).

Dr Wenxin Wang is trying to uncover therapies for diseases such as diabetic ulcers and Epidermolysis Bullosa, which causes chronic skin conditions: «We are currently investigating the use of these new materials for biomedical applications such as drug / gene delivery, cross linkable hydrogel materials and skin adhesives.

With the best - performing particles, the researchers reduced gene expression by more than 50 percent, for a dose of only 0.20 milligrams per kilogram of solution — about one - hundredth of the amount required with existing endothelial RNAi delivery vehicles.

This has limited the application of gene therapy, along with other concerns in the field, like the safety of the delivery vector.

Now, to enable widespread gene delivery throughout the central and peripheral nervous systems, Caltech researchers have developed two new variants of a vector based on an adeno - associated virus (AAV): one that can efficiently ferry genetic cargo past the blood - brain barrier; and another that is efficiently picked up by peripheral neurons residing outside the brain and spinal cord, such as those that sense pain and regulate heart rate, respiration, and digestion.

To confirm the links, the group harnessed cutting edge techniques in genetic engineering and viral gene delivery to switch on neurons in the front of healthy mouse brains.

Many drawbacks of viral gene delivery agents might be overcome by nonviral systems.

It would involve gene therapy delivered at the time of surgery and systemic delivery of the immune checkpoint inhibitors, such as through IV injection.

«We are hopeful that a systemic dose and delivery of gene therapy would broadly affect whole - body strength and function, and we are working on a host of projects for such systemic delivery in Pompe and other neuromuscular conditions,» said Barry Byrne, M.D., Ph.D., director of the Powell Gene Therapy Center, a professor of pediatrics in the UF College of Medicine, and a member of the UF Genetics Institgene therapy would broadly affect whole - body strength and function, and we are working on a host of projects for such systemic delivery in Pompe and other neuromuscular conditions,» said Barry Byrne, M.D., Ph.D., director of the Powell Gene Therapy Center, a professor of pediatrics in the UF College of Medicine, and a member of the UF Genetics InstitGene Therapy Center, a professor of pediatrics in the UF College of Medicine, and a member of the UF Genetics Institute.

This block is caused by mutations of the gene encoding the γc cytokine receptor subunit of interleukin - 2, -4, -7, -9, and -15 receptors, which participates in the delivery of growth, survival, and differentiation signals to early lymphoid progenitors.

Yehoash Raphael, an auditory neuroscientist at the University of Michigan, Ann Arbor, says the findings provide a new delivery model for researchers trying to use developmental genes to restore lost hearing.

There is a size limit in terms of what you can package into the gene delivery vehicle, and otoferlin is too large.

In addition to being the vector of choice for gene delivery to a variety of cell types, now you know that AAVs can provide a powerful boost to genome editing experiments.

The advent of molecular cloning, DNA sequencing and the many tools of molecular genetics and cell biology has given us sufficient knowledge of the basis for disease and the genes to target, but what has limited the application of gene therapy has been efficient gene delivery systems.

Asuri, Prashanth, et al. «Directed evolution of adeno - associated virus for enhanced gene delivery and gene targeting in human pluripotent stem cells.»

Enhancing muscle membrane repair by gene delivery of MG53 ameliorates muscular dystrophy and heart failure in d - Sarcoglycan - deficient hamsters.

It is hoped that the delivery of the missing SGSH gene provides a permanent source of functional enzyme in the brain that reverses phenotypic abnormalities of CNS neural cells.

It refers to a practical delivery system for the gene of your choice.

To address the editing challenges, we have developed a system that is based on the delivery of recombinant Cas9 protein complexed with an sgRNA targeting the gene of interest (Cas9 / sgRNA ribonucleoproteins [RNPs]-RRB- via cell - derived nanovesicles, called gesicles.

As a result, they are of great interest for fields such as biological imaging, medical diagnostics, drug delivery, and gene therapy.

Request a custom bibliography search or select from our list of citations available on - line: Select one Agents Administered Biotechnology / Protein & Peptide Delivery Cancer Research Cardiovascular Research Endocrinology Gene Therapy Immunology Neuroscience Pharmacology Physiology Route of Agent Administration Toxicology / Teratology In Vitro Use Nude Mice Neonates

Designer gene delivery vectors: molecular engineering and evolution of adeno - associated viral vectors for enhanced gene transfer.

I don't believe that the problem of in vivo gene delivery by vector has been solved.

Characterization of Human Serum Albumin - Facilitated Lipofection Gene Delivery Strategy.

Neurons of the peripheral nervous system (PNS), which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study of pain.

«The failure rate to deliver drugs to CNS is unfortunately very high, so any new methods of drug, protein and gene delivery should be welcome,» says Inder Verma, Ph.D., a professor in the Laboratory of Genetics and senior author of the study published in the Proceedings of the National Academy of Sciences.

Serap Günes (TUD)-- «Modification of vesicular stomatitis virus G protein for targeted gene delivery into PSCA - positive tumor cells» (2007)

This new study tested delivery of SUMO - 1 gene therapy alone, SERCA2 gene therapy alone, and a combination of SUMO - 1 and SERCA2.

In large animal models of heart failure, the researchers found that gene therapy delivery of high dose SUMO - 1 alone, as well as SUMO - 1 and SERCA2 together, result in stronger heart contractions, better blood flow, and reduced heart volumes, compared to just SERCA2 gene therapy alone.

To use viruses as delivery vehicles for the gene therapy, the researchers took all the harmful genes out of the virus and put in the beneficial genes in.

My laboratory has already developed molecules and gene transfer vectors that can target either the MAC, through the delivery of a protein called CD59, or the anaphylatoxins by delivery of proteases, or both by a protein called PRELP.

Synthesis and Characterization of Glycol Chitosan DNA Nanoparticles for Retinal Gene Delivery.

These features enable gesicles to knock out genes with high efficiency and in a broader range of cell types than plasmid - based delivery methods.

American and Danish scientists have now developed an active nanomotor for the efficient transport, delivery, and release of this gene scissoring system.

Then after that the next cycle of development will will focus on diversification of gene therapies, given standard practices for delivery and putting a new therapy into the market.