Sentences with phrase «of gene disruption»

Not exact matches

The disruption of prenatal cellular activity in zebra fish, which share 80 percent of their genes with humans and are considered a good model for studying human brain development, seemed to result in hyperactivity, according to the Canadian study, which was published Monday in the Proceedings of the National Academy of Sciences.

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

Our data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months time, paving the way to a humanized monoclonal antibody platform and additional human disease models.

To determine if defects in the atrial natriuretic peptide (ANP) system can cause hypertension, mice were generated with a disruption of the proANP gene.

But males have only one X chromosome, and therefore just one copy of the gene, so any disruption will turn them yellow.

«Our findings show that the gene mutation that causes Werner syndrome results in the disorganization of heterochromatin, and that this disruption of normal DNA packaging is a key driver of aging,» says Juan Carlos Izpisua Belmonte, a senior author on the paper.

Through temporary disruption of these interactions, the scientists were able to produce Drosophila epilines characterized by different levels of PcG - dependent gene repression or activation.

Disruptions to one or other of this gene cluster leads to distinctive mutations in which legs develop in place of antennae, for instance, as pictured here.

Rather than target a tumor - suppressor gene directly, Ideker and team took the approach of identifying genetic interactions between a tumor suppressor gene and another gene, such that simultaneous disruption of both genes selectively kills cancer cells.

Senior author on the paper, Deepak Srivastava, MD, director of cardiovascular and stem cell research at Gladstone and a pediatric cardiologist at the University of California San Francisco (UCSF), had previously discovered that disruption of one of two copies of a master gene called NOTCH1 can cause valve birth defects and CAVD.

Furthermore, gene disruption by gene editing techniques such as CRISPR - CAS resulted in the absence of growth of parasites carrying the mutated gene, indicating that the protein is required for parasite viability.

Even clearer evidence that disruptions in gene imprinting can undermine mental health comes from studies of Prader - Willi syndrome, a disorder that affects growth, sexual development and cognitive ability.

Mice generated to contain a targeted disruption of the DARPP - 32 gene showed profound deficits in their molecular, electrophysiological, and behavioral responses to dopamine, drugs of abuse, and antipsychotic medication.

Greenberg's lab also found that the disruption of long gene expression appears to be a distinctive signature of Rett Syndrome and related disorders.

«We identified that the peculiar look of these naked lizards is due to the disruption of the ectodysplasin - A (EDA), a gene whose mutations in humans and mice are known to generate substantial abnormalities in the development of teeth, glands, nails and hairs», says Michel Milinkovitch.

The new finding is the latest evidence supporting a growing precision medicine model of psychiatric disease in which disruptions of certain genes during brain development contribute to a person's risk for multiple psychiatric disorders, with other genetic or epigenetic drivers, random developmental events, or environmental influences determining the specific disease an individual develops, said senior author Benjamin Cheyette, MD, PhD, an associate professor of psychiatry and a member of the UCSF Weill Institute for Neurosciences and the Kavli Institute for Fundamental Neuroscience at UCSF.

Dr. Je and his team analysed signalling activity in neuronal cultures that either did not have the DTNBP1 gene or had lowered levels of the gene, because reduced DTNBP1 levels and genetic disruptions of DTNBP1 in mice resulted in schizophrenia - like behaviours.

«The disruption of the circadian clock machinery seems to affect not only the rhythm of appetite regulation, but also the expression of the genes and proteins at their absolute level,» says Turek, whose team reports its findings online today in Science.

An unexpected result of this disruption, however, was the fact that the antibiotic - resistant Escherichia coli bacteria that were trying to pass their genes along actually died when their DNA relaxase was shielded.

Beverly Emanuel, a human geneticist at the University of Pennsylvania in Philadelphia, notes that some DiGeorge patients have genetic disruptions at the suspect region but seem to have an intact UFD1 gene.

Differences in how genes are controlled, or even the loss or disruption of certain gene regulatory elements, may explain why human ancestors evolved to be so different from their great ape relatives.

This allowed them to identify those genes whose disruption was detrimental to the growth and survival of AML cells.

A major consequence of such interspecific hybridization may be outbreeding depression due to the break - up of co-adapted gene complexes and disruption of local adaptations (Barton & Hewitt 1989).

To begin to explore the cellular mechanisms that might be responsible for this response, histological analysis revealed less severe disruption of spermatogenesis and gene expression studies suggested that an interplay of ATP - binding cassette (ABC) efflux transporters and solute carrier (SLC) influx transporters in the testicular cells might be involved.

«We discovered gene - related disruption of circuits that connect the autistic brain's layers and hemispheres to each other,» explained Geschwind, who is director of the UCLA Neurogenetics Program and the Center for Autism Research and Treatment and co-director of the Center for Neurobehavioral Genetics at UCLA.

In the R5 - ZFN control group, with intact cxcr4 genes, 88 % of CD4 + T cells expressed CXCR4 protein at day 27 post engraftment, compared to 84 % of cells in the X4 - ZFN mice (∼ 24 % cxcr4 gene disruption) as determined by a fluorescence minus - one (FMO) control.

While CXCR4 gene disruption remained stable over time at approximately 30 %, CXCR4 gene disruption in HIV - infected cultures increased to 87 %, 91 %, and 88 % in the presence of BK132, HxB2, and R3A respectively after 21 days of infection.

Immune function and pathology dissected by high - throughput analysis of mice with targeted gene disruptions — an investigation by the Infection and Immunity Immunophenotyping (3i) consortium.

To delineate the role of Emk in development and adult tissues, mice lacking Emk were generated by targeted gene disruption.

disruption of the mechanisms that control the activation levels of different genes, leading to genes being wrongly activated or inactivated

Jeffrey Smith argues that the disruption of gene arrangements that occurs when a transgene is inserted will necessarily disturb regulatory networks that govern the way plants survive.

After acknowledging that there can be complicated interactions between genes, and that DNA insertion might influence regulatory networks involved in many genes, it also should be remembered that the original discovery of genes was only possible because disruption of a gene causes a limited set of changes to the organism, such as a change in eye colour.

A scheme of known expressed isoforms listed in WormBase WS246 and the strategy for the disruption of W01A8.1 gene is shown in Figs.

Targeted disruption of IRF - 1 or IRF - 2 results in abnormal type I IFN gene induction and aberrant lymphocyte development.

Azoospermia in mice with targeted disruption of the Brek / Lmtk2 (brain - enriched kinase / lemur tyrosine kinase 2) gene.

Virginia Tech researchers successfully used a gene disruption technique to change the eye color of a mosquito — a critical step toward new genetic strategies aimed at disrupting the transmission of diseases such as dengue fever.

Disruption of some genes accelerated M. genitalium growth.

The tools currently available to insert new genes permanently into cells do so at a random site inside the cell, which occasionally results in the disruption of the cell's native genes at the site of insertion, which could potentially turn the cell cancerous or cause other problems.

Genetic Alterations: Disruption caused by insertion of the retroviral gene trap vector ROSAFARY.

As part of its efforts to develop and apply tools for large - scale functional analysis, the BDGP has undertaken a novel gene disruption project based on mutagenesis by transposable element insertion (Spradling et al., 1995).

In MMEJ pathway, we achieve efficient gene disruption in human cell lines and animals by developing a computer program that assists the choice of nuclease target sites based on microhomology prediction.

Our previously described synthetic de-greening gene circuit shows a threshold - like response through generation of reactive oxygen species and photosystem disruption [7].

«Once we identify that disruption, we can begin exploring ways to restore normal gene function during early heart formation — and reduce the number of babies born with debilitating, and sometimes fatal, congenital heart defects.»

We interfere with histone modification marks in vivo, both genome - wide (mutant analysis) and gene - specifically (local disruption of chromatin structure), and analyze the effect on transcription and pluripotency.

There was a significant enrichment of gene expression disruption to pathways relevant in oxidative phosphorylation; and carbohydrate, amino acid, and lipid metabolism in diabetic co-twins.

The frequent proximity of the insertions to the 5» portion of the transcription unit means that the gene disruption in many lines could be molecularly identified by comparing the DNA sequence flanking the insertion to genomic sequence data.

We are currently testing the hypothesis that these transcription factors form a genomic regulatory network, and disruption of that network by mutation destabilizes gene expression programs, contributing to malignancy.

Scientists have for the first time shown how the disruption of a key gene involved in mental illness impacts on the brain.

Insect Allies» three technical areas — trait design, insect vector optimization, and selective gene therapy in mature plants — layer together to support the goal of rapidly transforming mature plants to protect against agricultural disruption without the need for extensive infrastructure.

Potential projects include identifying common pathways that modify retinal degenerative disease from a large collection of actively maintained mouse models; determining molecular networks implicated in pathological disruption of the retinal pigment epithelium; identifying molecular pathways that regulate postnatal ocular growth; and using mouse models to assess the pathogenic role of gene variants that increase the risk of age - related macular degeneration as identified by human genome - wide association studies.