Sentences with phrase «of gene targeting»

(A) Schematic representation of the gene targeting strategy.

Live at IMG Guillaume Pavlovic and Marie - Christine Birling, PHENOMIN - ICS, France Title: «Repertoire of gene targeting methods in rodents»

Ana got her PhD at ICGEB and Osaka University on strategies for optimization of HBsAg expression in Pichia pastoris under the direction of Dr. Navin Khanna, and she did postdoctoral work woth Prof. Rolf Jessberger at Mount Sinai School of Medicine in New York where she carried out a project aimed at deciphering the mechanisms of gene targeting by recombinant adeno - associated virus in human cells.

Homozygous lethality of the XPCS allele is likely due to reduced levels of expression of this essential protein as a result of gene targeting (Figure 1A) rather than to the mutation itself.

However, scientists are still barely scratching the surface of the full capabilities of gene targeting technology.

The marriage of Professors Capecchi and Smithies» homologous recombination technique with Sir Martin's stem cell discoveries has created the highly versatile new technology of gene targeting.

But CRISPR has proven so easy and inexpensive that Dana Carroll of the University of Utah, Salt Lake City, who spearheaded the development of zinc finger nucleases, says it has brought about the «democratization of gene targeting.»

«CRISPR has proven so easy and inexpensive that Dana Carroll of the University of Utah, Salt Lake City, who spearheaded the development of zinc finger nucleases, [one of its competitors,] says it has brought about the «democratization of gene targeting.

«We captured about 95 percent of the gene targets we set out to capture, many of which were very large in DNA length, which has been challenging in the past,» Parekkadan said.

Given all the drugs on the market, geneticist Kerry Kornfeld and his colleagues at Washington University in St. Louis, Missouri, speculated that some of these drugs might retard aging or promote longevity by affecting one of those gene targets.

Students use online databases - including WormBase and Pubmed - to explore the molecular genetics and physiological functions of the genes targeted by RNAi.

Not exact matches

That's not to say gene editing is new (it isn't), but Crispr simplifies the process by using molecular scissors that can be precisely targeted to snip out aberrant regions of genetic code, which can then be replaced with the correct sequences.

I won't reveal yet who my favorites are, but I will say that these young scientist - founders came up with very creative solutions for preventing infections in some common surgeries, tackling resistance in targeted antibody drugs, improving gene vectors for cell therapies, helping the vision - impaired «see» faces and better read their environments, imaging hard - to - see spots in the lungs and other organs, improving genetic risk analysis, and expediting the logistical operations of hospitals.

Luxturna is the first of a crop of treatments that target diseases caused by mutations in specific genes, and thus is referred to by many as the first gene therapy in the U.S.

Alnylam has been developing drugs that target gene expression in order to prevent to production of certain biological material which can cause devastating diseases.

Well, one startup is seeking to take a very different approach: Exonics Therapeutics, which has secured $ 5 million in seed funding from CureDuchenne Ventures to see if the revolutionary new CRISPR - Cas - 9 gene - editing technology can be used to target the root genetic deficiency at the heart of the disease.

The Kamens claim the main thing that distinguishes their foundation from other brain tumor foundations is their focus on pediatric brain cancer specifically, as well as their close ties with pharmaceutical and biotech companies working in the fields of immunotherapy and target gene therapy.

«Mar - 18 is the third out of three quarters that they have failed to meet this important target,» Loup Ventures managing partner Gene Munster said in a note.

During the dialogue, Dr Gene Nisperos of the Health Alliance for Democracy raised his concern as the target of Golden Rice are members of the vulnerable population such as preschool children and lactating mothers.

In the study, whole - exome sequencing and a targeted analysis of 90 genes implicated in heart disease were applied to 419 unrelated SIDS cases.

The finance chairman and a finance committee member of Gene Locke's mayoral campaign helped bankroll the conservative political action committee that sent out an anti-gay mailer targeting City Controller Annise Parker and other municipal candidates earlier this month, according to Texas Ethics Commission documents.

In addition, they also used exome sequencing and RNAseq — sequencing directly targeted to the SHIP1 gene — to examine the structure of the SHIP1 gene at both the DNA and the RNA level.

A local philanthropist underwrote purchase of a new Illumina MiSeq device that facilitates targeted gene sequencing.

To generate a rich source of potential vaccine and drug target candidates, the team identified a set of genes that are more active in certain stages of the parasite life cycle and within the parasite's gut.

Identifying groups of genes that work together, and then targeting these networks of genes, may lead to more effective treatments.

To discover these targets, the team determined when and where each gene is turned on or off in the cells and tissues of H. contortus to reveal new insights into the worm's lifecycle.

This permits high - resolution mapping of genes and chromosomal regions on single fibers of DNA, and it targets the physical location of mitochondrial DNA probes down to a resolution of 1,000 base pairs.

«The extensive overlap in risk genes for autism and cancer, many of which are chromatin remodeling factors, supports the idea of repurposing epigenetic drugs used in cancer treatment as targeted treatments for autism,» said Yan.

Some morphogenic gradients apparently yield but a single effect: If the concentration of a morphogen in a particular place is above a critical threshold, a target gene is activated; otherwise, it is not.

In SIF - seq, hundreds or thousands of DNA fragments to be tested for enhancer activity are coupled to a reporter gene and targeted into a single, reproducible site in embryonic cell genomes.

The study, published online today in Science Translational Medicine, further points to a master switch for these gene sets as a potential target of future therapies.

New research at the University at Buffalo reveals the first evidence that it may be possible to use a single compound to alleviate the behavioral symptoms by targeting sets of genes involved in the disease.

If Rudenko confirms Clark's PCR tests using additional gene targets, and if Clark or Rudenko manage to grow Borrelia cultures out of human and lone star tick samples, then even the fiercest skeptics will have to recognize that Southern Lyme strains threaten human health, and that Lyme - like illness deserves Lyme - like treatment.

In all the pathways so far investigated, the final result is a gradient of morphogen that functions principally as a transcription factor, initiating or suppressing the transcription of one or more target genes in a concentration - dependent manner.

When a target gene is unmethylated, the reporter is also unmethylated, triggering expression of a glowing protein that illuminates the cell.

Women with precancer or cancer were most likely to have particular versions of the viral gene E7, which researchers are now interested in targeting.

Since it is proteins that do the reprogramming, rather than the genes that make them, the researchers reasoned that they simply needed to get enough of the proteins into the target cells.

Recent advances in the understanding of cancer have led to more personalized therapies, such as drugs that target particular proteins and tests that analyze gene expression patterns in tumors to predict a patient's response to therapy.

They note that targeting inflammation to treat infections offers an advantage over antibiotic therapy, as the former hinders gene transfer and the evolution of pathogens, while the latter promotes bacterial evolution and, ultimately, antibiotic resistance.

The Bill and Melinda Gates Foundation sees so much promise in gene drive technology that it plans to double spending on its Target Malaria initiative, which aims to create systems for driving genes in two species of malaria mosquitoes, to $ 70 million.

«Many diseases, especially complex diseases, involve multiple genes, and this system could be used therapeutically to target and activate multiple genes together and rescue these disease phenotypes,» says Albert Cheng, a graduate student in the Jaenisch lab and co-author of the Cell Research paper.

«We spent half a dozen years trying to target the beta globin gene using the old technology,» he noted, adding that within one week of trying CRISPR, they had an editing tool that worked much better.

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

The gene, called NPC1L1, is of interest because it is the target of the drug ezetimibe, often prescribed to lower cholesterol.

Using this process, scientists can make targeted mutations in the genomes of living animals, either deleting genes or inserting new ones.

«This structural «footprint» we found seems to help these viruses get efficiently into the brain, which informs the design of potentially safer brain - targeted gene therapies,» said study senior author Aravind Asokan, PhD, associate professor of genetics.

Our data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months time, paving the way to a humanized monoclonal antibody platform and additional human disease models.

Carlo Croce, a cancer researcher at Ohio State University in Columbus, and his colleagues created a diagram of interacting miRNAs for normal body cells by connecting them according to which genes they target and the function of those genes, in a way similar to analyses of human social networks.

«But we can say this genetic analysis gives us some confidence that targeting this gene should reduce the risk of heart attack.

«The state of the art right now is targeting two or three genes simultaneously and then looking at the effects, but we think that perhaps the gene sets that need to be modulated to address some of these diseases are actually broader than that,» says Lu, who is the senior author of the study.